(Linear) (Six-base) MAPSORT of: KIEE0851.seq  Check: 1227  from: 1  to: 903



KIEE0851.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     700     903 
   Size:        700     203
 
AleI CACnn'nnGTG

Cuts at:      0     273     903 
   Size:        273     630
 
ApoI r'AATT_y

Cuts at:      0       4     122     458     903 
   Size:          4     118     336     445

  Fragments arranged by size: 

                 445     336     118       4
 
AseI AT'TA_AT

Cuts at:      0     494     903 
   Size:        494     409
 
BanI G'GyrC_C

Cuts at:      0     597     903 
   Size:        597     306
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     377     903 
   Size:        377     526
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121     903 
   Size:        121     782
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     511     903 
   Size:        511     392
 
BsaAI yAC'GTr

Cuts at:      0     342     903 
   Size:        342     561
 
BsmI GAATG_Cn'

Cuts at:      0     218     395     903 
   Size:        218     177     508

  Fragments arranged by size: 

                 508     218     177
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     700     724     903 
   Size:        700      24     179

  Fragments arranged by size: 

                 700     179      24
 
BsrBI CCG'CTC

Cuts at:      0     133     903 
   Size:        133     770
 
BsrDI GCAATG_nn'

Cuts at:      0     102     903 
   Size:        102     801
 
Bsu36I CC'TnA_GG

Cuts at:      0     847     903 
   Size:        847      56
 
DraIII CAC_nnn'GTG

Cuts at:      0     305     903 
   Size:        305     598
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121     903 
   Size:        121     782
 
EcoRV GAT'ATC

Cuts at:      0     861     903 
   Size:        861      42
 
HindIII A'AGCT_T

Cuts at:      0     529     903 
   Size:        529     374
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     665     844     903 
   Size:        665     179      59

  Fragments arranged by size: 

                 665     179      59
 
MslI CAynn'nnrTG

Cuts at:      0     273     550     903 
   Size:        273     277     353

  Fragments arranged by size: 

                 353     277     273
 
MspA1I CmG'CkG

Cuts at:      0     402     903 
   Size:        402     501
 
NspI r_CATG'y

Cuts at:      0     455     903 
   Size:        455     448
 
PfoI T'CCnGG_A

Cuts at:      0     415     903 
   Size:        415     488
 
PvuII CAG'CTG

Cuts at:      0     402     903 
   Size:        402     501
 
SfcI C'TryA_G

Cuts at:      0     434     903 
   Size:        434     469
 
SmlI C'TyrA_G

Cuts at:      0     526     903 
   Size:        526     377
 
SspI AAT'ATT

Cuts at:      0     246     903 
   Size:        246     657
 
XmnI GAAnn'nnTTC

Cuts at:      0       8     246     903 
   Size:          8     238     657

  Fragments arranged by size: 

                 657     238       8
 

 Enzymes that do cut:

     AarI     AleI     ApoI     AseI     BanI    BciVI     BpmI    BpuEI
    BsaAI     BsmI    BspMI    BsrBI    BsrDI   Bsu36I   DraIII  Eco57MI
    EcoRV  HindIII     MmeI     MslI   MspA1I     NspI     PfoI    PvuII
     SfcI     SmlI     SspI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI
    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
    BsmBI Bsp1286I    BspEI    BspHI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI