(Linear) (Six-base) MAPSORT of: KIEE0849.seq  Check: 9799  from: 1  to: 948



KIEE0849.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

Acc65I G'GTAC_C

Cuts at:      0     292     948 
   Size:        292     656
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     614     948 
   Size:        614     334
 
ApoI r'AATT_y

Cuts at:      0      31     948 
   Size:         31     917
 
AvaI C'yCGr_G

Cuts at:      0      61     948 
   Size:         61     887
 
BanI G'GyrC_C

Cuts at:      0     231     292     948 
   Size:        231      61     656

  Fragments arranged by size: 

                 656     231      61
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     564     948 
   Size:        564     384
 
BglII A'GATC_T

Cuts at:      0     249     948 
   Size:        249     699
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     541     948 
   Size:        541     407
 
BsaWI w'CCGG_w

Cuts at:      0     451     948 
   Size:        451     497
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     785     948 
   Size:        785     163
 
BstYI r'GATC_y

Cuts at:      0     153     249     948 
   Size:        153      96     699

  Fragments arranged by size: 

                 699     153      96
 
Bsu36I CC'TnA_GG

Cuts at:      0     874     948 
   Size:        874      74
 
BtgI C'CryG_G

Cuts at:      0     518     948 
   Size:        518     430
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     500     535     948 
   Size:        500      35     413

  Fragments arranged by size: 

                 500     413      35
 
EaeI y'GGCC_r

Cuts at:      0      95     371     431     696     948 
   Size:         95     276      60     265     252

  Fragments arranged by size: 

                 276     265     252      95      60
 
EarI CTCTTCn'nnn_

Cuts at:      0     754     948 
   Size:        754     194
 
EcoO109I rG'GnC_Cy

Cuts at:      0     413     948 
   Size:        413     535
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     241     273     900     932     948 
   Size:        241      32     627      32      16

  Fragments arranged by size: 

                 627     241      32      32      16
 
KpnI G_GTAC'C

Cuts at:      0     296     948 
   Size:        296     652
 
MscI TGG'CCA

Cuts at:      0      97     373     433     948 
   Size:         97     276      60     515

  Fragments arranged by size: 

                 515     276      97      60
 
MslI CAynn'nnrTG

Cuts at:      0     739     847     948 
   Size:        739     108     101

  Fragments arranged by size: 

                 739     108     101
 
PpuMI rG'GwC_Cy

Cuts at:      0     413     948 
   Size:        413     535
 
SfcI C'TryA_G

Cuts at:      0     472     948 
   Size:        472     476
 
SpeI A'CTAG_T

Cuts at:      0     558     948 
   Size:        558     390
 
StyI C'CwwG_G

Cuts at:      0     221     262     948 
   Size:        221      41     686

  Fragments arranged by size: 

                 686     221      41
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     186     948 
   Size:        186     762
 
TatI w'GTAC_w

Cuts at:      0     176     302     948 
   Size:        176     126     646

  Fragments arranged by size: 

                 646     176     126
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     438     470     948 
   Size:        438      32     478

  Fragments arranged by size: 

                 478     438      32
 
XmnI GAAnn'nnTTC

Cuts at:      0      35     948 
   Size:         35     913
 

 Enzymes that do cut:

   Acc65I     AlfI     ApoI     AvaI     BanI    BciVI    BglII     BmrI
    BsaWI    BspMI    BstYI   Bsu36I     BtgI    CspCI     EaeI     EarI
 EcoO109I    Hin4I     KpnI     MscI     MslI    PpuMI     SfcI     SpeI
     StyI    TaqII     TatI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI
    BbvCI     BcgI     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
    BtgZI     BtsI     ClaI     DraI   DraIII     DrdI     EagI     EciI
  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     MfeI     MluI
     MmeI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SphI     SrfI     SspI     StuI
     SwaI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI