(Linear) (Six-base) MAPSORT of: KIEE0848.seq  Check: 6271  from: 1  to: 942



KIEE0848.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     697     942 
   Size:        697     245
 
AflIII A'CryG_T

Cuts at:      0     572     942 
   Size:        572     370
 
AleI CACnn'nnGTG

Cuts at:      0     739     942 
   Size:        739     203
 
ApoI r'AATT_y

Cuts at:      0      31     942 
   Size:         31     911
 
AseI AT'TA_AT

Cuts at:      0     430     942 
   Size:        430     512
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     631     664     942 
   Size:        631      33     278

  Fragments arranged by size: 

                 631     278      33
 
BanI G'GyrC_C

Cuts at:      0     604     942 
   Size:        604     338
 
BanII G_rGCy'C

Cuts at:      0      68     942 
   Size:         68     874
 
BfrBI ATG'CAT

Cuts at:      0     595     942 
   Size:        595     347
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     704     942 
   Size:        704     238
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     942 
   Size:         52     890
 
Bpu10I CC'TnA_GC

Cuts at:      0      62     942 
   Size:         62     880
 
BsaWI w'CCGG_w

Cuts at:      0     801     942 
   Size:        801     141
 
BseYI C'CCAG_C

Cuts at:      0     543     942 
   Size:        543     399
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     242     587     942 
   Size:        242     345     355

  Fragments arranged by size: 

                 355     345     242
 
BsiHKAI G_wGCw'C

Cuts at:      0      68     942 
   Size:         68     874
 
BsmI GAATG_Cn'

Cuts at:      0     595     942 
   Size:        595     347
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     150     942 
   Size:        150     792
 
Bsp1286I G_dGCh'C

Cuts at:      0      68     942 
   Size:         68     874
 
BsrBI CCG'CTC

Cuts at:      0     727     942 
   Size:        727     215
 
BstEII G'GTnAC_C

Cuts at:      0     108     942 
   Size:        108     834
 
Bsu36I CC'TnA_GG

Cuts at:      0     450     942 
   Size:        450     492
 
BtsI GCAGTG_nn'

Cuts at:      0     554     847     942 
   Size:        554     293      95

  Fragments arranged by size: 

                 554     293      95
 
EarI CTCTTCn'nnn_

Cuts at:      0     886     942 
   Size:        886      56
 
EcoICRI GAG'CTC

Cuts at:      0      66     942 
   Size:         66     876
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     697     942 
   Size:         52     645     245

  Fragments arranged by size: 

                 645     245      52
 
EcoRI G'AATT_C

Cuts at:      0      31     942 
   Size:         31     911
 
MslI CAynn'nnrTG

Cuts at:      0     739     942 
   Size:        739     203
 
NsiI A_TGCA'T

Cuts at:      0     597     942 
   Size:        597     345
 
NspI r_CATG'y

Cuts at:      0     566     942 
   Size:        566     376
 
SacI G_AGCT'C

Cuts at:      0      68     942 
   Size:         68     874
 
SfcI C'TryA_G

Cuts at:      0     867     942 
   Size:        867      75
 
StuI AGG'CCT

Cuts at:      0     709     942 
   Size:        709     233
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     377     942 
   Size:        377     565
 
XmnI GAAnn'nnTTC

Cuts at:      0     335     942 
   Size:        335     607
 

 Enzymes that do cut:

     AcuI   AflIII     AleI     ApoI     AseI     BaeI     BanI    BanII
    BfrBI     BmrI     BpmI   Bpu10I    BsaWI    BseYI     BsgI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BsrBI   BstEII   Bsu36I     BtsI     EarI
  EcoICRI  Eco57MI    EcoRI     MslI     NsiI     NspI     SacI     SfcI
     StuI    TaqII     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI
     AvaI    AvrII    BamHI     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BsiEI
    BsiWI    BspEI    BspHI    BspMI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI    BstXI    BstYI  BstZ17I     BtgI    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StyI
     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI