(Linear) (Six-base) MAPSORT of: KIEE0846.seq  Check: 538  from: 1  to: 942



KIEE0846.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     697     942 
   Size:        697     245
 
AflIII A'CryG_T

Cuts at:      0     833     942 
   Size:        833     109
 
AleI CACnn'nnGTG

Cuts at:      0     739     942 
   Size:        739     203
 
ApoI r'AATT_y

Cuts at:      0      31     942 
   Size:         31     911
 
AseI AT'TA_AT

Cuts at:      0     521     942 
   Size:        521     421
 
BanI G'GyrC_C

Cuts at:      0     604     942 
   Size:        604     338
 
BanII G_rGCy'C

Cuts at:      0      68     942 
   Size:         68     874
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     391     704     942 
   Size:        391     313     238

  Fragments arranged by size: 

                 391     313     238
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     942 
   Size:         52     890
 
Bpu10I CC'TnA_GC

Cuts at:      0      62     942 
   Size:         62     880
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      94     242     493     587     942 
   Size:         94     148     251      94     355

  Fragments arranged by size: 

                 355     251     148      94      94
 
BsiHKAI G_wGCw'C

Cuts at:      0      68     254     942 
   Size:         68     186     688

  Fragments arranged by size: 

                 688     186      68
 
Bsp1286I G_dGCh'C

Cuts at:      0      68     254     942 
   Size:         68     186     688

  Fragments arranged by size: 

                 688     186      68
 
BsrBI CCG'CTC

Cuts at:      0     861     942 
   Size:        861      81
 
BstEII G'GTnAC_C

Cuts at:      0     108     942 
   Size:        108     834
 
BstYI r'GATC_y

Cuts at:      0     446     942 
   Size:        446     496
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     364     942 
   Size:        364     578
 
EarI CTCTTCn'nnn_

Cuts at:      0     535     886     942 
   Size:        535     351      56

  Fragments arranged by size: 

                 535     351      56
 
EcoICRI GAG'CTC

Cuts at:      0      66     942 
   Size:         66     876
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     697     942 
   Size:         52     645     245

  Fragments arranged by size: 

                 645     245      52
 
EcoRI G'AATT_C

Cuts at:      0      31     942 
   Size:         31     911
 
MslI CAynn'nnrTG

Cuts at:      0     739     942 
   Size:        739     203
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     505     942 
   Size:        505     437
 
SacI G_AGCT'C

Cuts at:      0      68     942 
   Size:         68     874
 
SfcI C'TryA_G

Cuts at:      0     867     942 
   Size:        867      75
 
SspI AAT'ATT

Cuts at:      0     437     942 
   Size:        437     505
 
StuI AGG'CCT

Cuts at:      0     709     942 
   Size:        709     233
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     377     942 
   Size:        377     565
 
XmnI GAAnn'nnTTC

Cuts at:      0     335     942 
   Size:        335     607
 

 Enzymes that do cut:

     AcuI   AflIII     AleI     ApoI     AseI     BanI    BanII     BmrI
     BpmI   Bpu10I     BsgI  BsiHKAI Bsp1286I    BsrBI   BstEII    BstYI
    BtgZI     EarI  EcoICRI  Eco57MI    EcoRI     MslI    PflMI     SacI
     SfcI     SspI     StuI    TaqII     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI    BstXI  BstZ17I   Bsu36I     BtgI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI    EcoNI EcoO109I
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     StyI     SwaI     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI