Restriction map for
pF1KE0844
(Linear) (Six-base) MAPSORT of: KIEE0844.seq Check: 1144 from: 1 to: 903
KIEE0844.orf
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 301 903
Size: 301 602
AccI GT'mk_AC
Cuts at: 0 875 903
Size: 875 28
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 791 903
Size: 791 112
AleI CACnn'nnGTG
Cuts at: 0 724 903
Size: 724 179
AlwNI CAG_nnn'CTG
Cuts at: 0 534 903
Size: 534 369
BanI G'GyrC_C
Cuts at: 0 286 903
Size: 286 617
BanII G_rGCy'C
Cuts at: 0 581 903
Size: 581 322
BbsI GAAGACnn'nnnn_
Cuts at: 0 300 722 903
Size: 300 422 181
Fragments arranged by size:
422 300 181
BciVI GTATCCnnnnn_n'
Cuts at: 0 487 903
Size: 487 416
BclI T'GATC_A
Cuts at: 0 317 903
Size: 317 586
BmrI ACTGGGnnnn_n'
Cuts at: 0 113 863 903
Size: 113 750 40
Fragments arranged by size:
750 113 40
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 315 903
Size: 315 588
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 321 903
Size: 321 582
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 727 757 903
Size: 727 30 146
Fragments arranged by size:
727 146 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 84 129 601 903
Size: 84 45 472 302
Fragments arranged by size:
472 302 84 45
BsiHKAI G_wGCw'C
Cuts at: 0 721 903
Size: 721 182
Bsp1286I G_dGCh'C
Cuts at: 0 581 721 903
Size: 581 140 182
Fragments arranged by size:
581 182 140
BspHI T'CATG_A
Cuts at: 0 854 903
Size: 854 49
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 101 301 903
Size: 101 200 602
Fragments arranged by size:
602 200 101
BsrDI GCAATG_nn'
Cuts at: 0 548 903
Size: 548 355
BstAPI GCAn_nnn'nTGC
Cuts at: 0 107 293 903
Size: 107 186 610
Fragments arranged by size:
610 186 107
BstZ17I GTA'TAC
Cuts at: 0 876 903
Size: 876 27
BtgI C'CryG_G
Cuts at: 0 44 602 903
Size: 44 558 301
Fragments arranged by size:
558 301 44
BtsI GCAGTG_nn'
Cuts at: 0 26 532 903
Size: 26 506 371
Fragments arranged by size:
506 371 26
DraIII CAC_nnn'GTG
Cuts at: 0 107 538 903
Size: 107 431 365
Fragments arranged by size:
431 365 107
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 315 791 903
Size: 315 476 112
Fragments arranged by size:
476 315 112
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 206 238 903
Size: 206 32 665
Fragments arranged by size:
665 206 32
MslI CAynn'nnrTG
Cuts at: 0 442 475 712 724 903
Size: 442 33 237 12 179
Fragments arranged by size:
442 237 179 33 12
MspA1I CmG'CkG
Cuts at: 0 173 633 903
Size: 173 460 270
Fragments arranged by size:
460 270 173
NcoI C'CATG_G
Cuts at: 0 602 903
Size: 602 301
NspI r_CATG'y
Cuts at: 0 379 417 903
Size: 379 38 486
Fragments arranged by size:
486 379 38
PflMI CCAn_nnn'nTGG
Cuts at: 0 195 264 903
Size: 195 69 639
Fragments arranged by size:
639 195 69
PfoI T'CCnGG_A
Cuts at: 0 331 903
Size: 331 572
SmlI C'TyrA_G
Cuts at: 0 300 903
Size: 300 603
StyI C'CwwG_G
Cuts at: 0 602 903
Size: 602 301
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 95 224 903
Size: 95 129 679
Fragments arranged by size:
679 129 95
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 792 903
Size: 792 111
Enzymes that do cut:
AarI AccI AcuI AleI AlwNI BanI BanII BbsI
BciVI BclI BmrI BpmI BpuEI BsaXI BsgI BsiHKAI
Bsp1286I BspHI BspMI BsrDI BstAPI BstZ17I BtgI BtsI
DraIII Eco57MI Hin4I MslI MspA1I NcoI NspI PflMI
PfoI SmlI StyI TaqII XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmtI BplI Bpu10I
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiWI BsmI BsmBI BspEI BsrBI BsrFI BsrGI BssHII
BssSI BstBI BstEII BstXI BstYI Bsu36I BtgZI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI