(Linear) (Six-base) MAPSORT of: KIEE0843.seq  Check: 8015  from: 1  to: 1059



KIEE0843.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     455    1059 
   Size:        455     604
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      62    1059 
   Size:         62     997
 
ApaI G_GGCC'C

Cuts at:      0     531    1059 
   Size:        531     528
 
ApoI r'AATT_y

Cuts at:      0     186    1059 
   Size:        186     873
 
BamHI G'GATC_C

Cuts at:      0     176    1059 
   Size:        176     883
 
BanII G_rGCy'C

Cuts at:      0     531     841    1059 
   Size:        531     310     218

  Fragments arranged by size: 

                 531     310     218
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     720    1059 
   Size:        720     339
 
BbvCI CC'TCA_GC

Cuts at:      0     406    1059 
   Size:        406     653
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     299     610    1059 
   Size:        299     311     449

  Fragments arranged by size: 

                 449     311     299
 
BfrBI ATG'CAT

Cuts at:      0     550    1059 
   Size:        550     509
 
Bme1580I G_kGCm'C

Cuts at:      0     394     531    1059 
   Size:        394     137     528

  Fragments arranged by size: 

                 528     394     137
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     449    1059 
   Size:        449     610
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     421    1004    1059 
   Size:        421     583      55

  Fragments arranged by size: 

                 583     421      55
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     188    1059 
   Size:        188     871
 
Bpu10I CC'TnA_GC

Cuts at:      0     406    1059 
   Size:        406     653
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     234    1059 
   Size:        234     825
 
BsaBI GATnn'nnATC

Cuts at:      0     475    1059 
   Size:        475     584
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     766     796    1059 
   Size:        766      30     263

  Fragments arranged by size: 

                 766     263      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     508    1059 
   Size:        508     551
 
BseYI C'CCAG_C

Cuts at:      0     524     951    1059 
   Size:        524     427     108

  Fragments arranged by size: 

                 524     427     108
 
BsiHKAI G_wGCw'C

Cuts at:      0     841    1059 
   Size:        841     218
 
BsmI GAATG_Cn'

Cuts at:      0     552    1059 
   Size:        552     507
 
Bsp1286I G_dGCh'C

Cuts at:      0     394     531     841    1059 
   Size:        394     137     310     218

  Fragments arranged by size: 

                 394     310     218     137
 
BspHI T'CATG_A

Cuts at:      0     977    1059 
   Size:        977      82
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     455    1059 
   Size:        455     604
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     416    1059 
   Size:        416     643
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     911    1059 
   Size:        911     148
 
BstYI r'GATC_y

Cuts at:      0     176     487    1059 
   Size:        176     311     572

  Fragments arranged by size: 

                 572     311     176
 
BtgI C'CryG_G

Cuts at:      0     245     284     722    1059 
   Size:        245      39     438     337

  Fragments arranged by size: 

                 438     337     245      39
 
DraI TTT'AAA

Cuts at:      0     300    1059 
   Size:        300     759
 
DraIII CAC_nnn'GTG

Cuts at:      0     387    1059 
   Size:        387     672
 
EcoICRI GAG'CTC

Cuts at:      0     839    1059 
   Size:        839     220
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      62     188    1059 
   Size:         62     126     871

  Fragments arranged by size: 

                 871     126      62
 
EcoO109I rG'GnC_Cy

Cuts at:      0     528    1059 
   Size:        528     531
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     387    1059 
   Size:        387     672
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     161     193    1059 
   Size:        161      32     866

  Fragments arranged by size: 

                 866     161      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     783    1059 
   Size:        783     276
 
MslI CAynn'nnrTG

Cuts at:      0     823    1059 
   Size:        823     236
 
NcoI C'CATG_G

Cuts at:      0     284     722    1059 
   Size:        284     438     337

  Fragments arranged by size: 

                 438     337     284
 
NsiI A_TGCA'T

Cuts at:      0     552    1059 
   Size:        552     507
 
NspI r_CATG'y

Cuts at:      0     661    1059 
   Size:        661     398
 
PsiI TTA'TAA

Cuts at:      0     209    1059 
   Size:        209     850
 
PspOMI G'GGCC_C

Cuts at:      0     527    1059 
   Size:        527     532
 
PstI C_TGCA'G

Cuts at:      0     790    1018    1059 
   Size:        790     228      41

  Fragments arranged by size: 

                 790     228      41
 
SacI G_AGCT'C

Cuts at:      0     841    1059 
   Size:        841     218
 
SbfI CC_TGCA'GG

Cuts at:      0    1018    1059 
   Size:       1018      41
 
SfcI C'TryA_G

Cuts at:      0     738     786     890    1014    1059 
   Size:        738      48     104     124      45

  Fragments arranged by size: 

                 738     124     104      48      45
 
SspI AAT'ATT

Cuts at:      0     340    1059 
   Size:        340     719
 
StyI C'CwwG_G

Cuts at:      0     272     284     493     722    1059 
   Size:        272      12     209     229     337

  Fragments arranged by size: 

                 337     272     229     209      12
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     720     828     930    1059 
   Size:        720     108     102     129

  Fragments arranged by size: 

                 720     129     108     102
 

 Enzymes that do cut:

     AarI     AcuI     ApaI     ApoI    BamHI    BanII     BbsI    BbvCI
    BciVI    BfrBI Bme1580I     BmrI     BplI     BpmI   Bpu10I     BsaI
    BsaBI    BsaXI    BseRI    BseYI  BsiHKAI     BsmI Bsp1286I    BspHI
    BspMI   BstAPI    BstXI    BstYI     BtgI     DraI   DraIII  EcoICRI
  Eco57MI EcoO109I     FalI    Hin4I     MmeI     MslI     NcoI     NsiI
     NspI     PsiI   PspOMI     PstI     SacI     SbfI     SfcI     SspI
     StyI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI     BanI     BbeI     BcgI     BclI
     BglI    BglII     BlpI    BmgBI     BmtI    BpuEI    BsaAI    BsaHI
    BsaWI     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DrdI     EaeI     EagI     EarI
     EciI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI    PspXI     PsrI     PvuI    PvuII    RsrII    SacII
     SalI    SanDI     SapI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI