(Linear) (Six-base) MAPSORT of: KIEE0838.seq  Check: 4728  from: 1  to: 927



KIEE0838.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     310     927 
   Size:        310     617
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     346     385     927 
   Size:        346      39     542

  Fragments arranged by size: 

                 542     346      39
 
AflIII A'CryG_T

Cuts at:      0     901     927 
   Size:        901      26
 
ApaLI G'TGCA_C

Cuts at:      0     388     927 
   Size:        388     539
 
ApoI r'AATT_y

Cuts at:      0      46     164     927 
   Size:         46     118     763

  Fragments arranged by size: 

                 763     118      46
 
BamHI G'GATC_C

Cuts at:      0     395     927 
   Size:        395     532
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     306     927 
   Size:        306     621
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      67     927 
   Size:         67     860
 
BclI T'GATC_A

Cuts at:      0     173     927 
   Size:        173     754
 
BglII A'GATC_T

Cuts at:      0     616     927 
   Size:        616     311
 
Bme1580I G_kGCm'C

Cuts at:      0     392     927 
   Size:        392     535
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      62     927 
   Size:         62     865
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     662     927 
   Size:        662     265
 
BseYI C'CCAG_C

Cuts at:      0     402     673     927 
   Size:        402     271     254

  Fragments arranged by size: 

                 402     271     254
 
BsiHKAI G_wGCw'C

Cuts at:      0     392     927 
   Size:        392     535
 
BsmI GAATG_Cn'

Cuts at:      0     899     927 
   Size:        899      28
 
Bsp1286I G_dGCh'C

Cuts at:      0     392     927 
   Size:        392     535
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     310     927 
   Size:        310     617
 
BsrDI GCAATG_nn'

Cuts at:      0      77     927 
   Size:         77     850
 
BsrFI r'CCGG_y

Cuts at:      0     658     927 
   Size:        658     269
 
BstYI r'GATC_y

Cuts at:      0     395     616     927 
   Size:        395     221     311

  Fragments arranged by size: 

                 395     311     221
 
DraI TTT'AAA

Cuts at:      0     863     927 
   Size:        863      64
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      62     346     385     927 
   Size:         62     284      39     542

  Fragments arranged by size: 

                 542     284      62      39
 
EcoRI G'AATT_C

Cuts at:      0      46     927 
   Size:         46     881
 
EcoRV GAT'ATC

Cuts at:      0     596     927 
   Size:        596     331
 
HincII GTy'rAC

Cuts at:      0      90     132     927 
   Size:         90      42     795

  Fragments arranged by size: 

                 795      90      42
 
MfeI C'AATT_G

Cuts at:      0     479     569     927 
   Size:        479      90     358

  Fragments arranged by size: 

                 479     358      90
 
NspI r_CATG'y

Cuts at:      0     905     927 
   Size:        905      22
 
PciI A'CATG_T

Cuts at:      0     901     927 
   Size:        901      26
 
PshAI GACnn'nnGTC

Cuts at:      0     886     927 
   Size:        886      41
 
PsiI TTA'TAA

Cuts at:      0      28     927 
   Size:         28     899
 
PstI C_TGCA'G

Cuts at:      0      15     927 
   Size:         15     912
 
ScaI AGT'ACT

Cuts at:      0     209     452     927 
   Size:        209     243     475

  Fragments arranged by size: 

                 475     243     209
 
SfcI C'TryA_G

Cuts at:      0      11     927 
   Size:         11     916
 
SpeI A'CTAG_T

Cuts at:      0      84     927 
   Size:         84     843
 
SspI AAT'ATT

Cuts at:      0      62     927 
   Size:         62     865
 
StyI C'CwwG_G

Cuts at:      0     833     927 
   Size:        833      94
 
TatI w'GTAC_w

Cuts at:      0     207     450     927 
   Size:        207     243     477

  Fragments arranged by size: 

                 477     243     207
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     356     423     927 
   Size:        356      67     504

  Fragments arranged by size: 

                 504     356      67
 

 Enzymes that do cut:

     AarI     AcuI   AflIII    ApaLI     ApoI    BamHI     BbsI    BciVI
     BclI    BglII Bme1580I     BpmI     BsaI    BseYI  BsiHKAI     BsmI
 Bsp1286I    BspMI    BsrDI    BsrFI    BstYI     DraI  Eco57MI    EcoRI
    EcoRV   HincII     MfeI     NspI     PciI    PshAI     PsiI     PstI
     ScaI     SfcI     SpeI     SspI     StyI     TatI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI     BanI    BanII     BbeI    BbvCI     BcgI
    BfrBI     BglI     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I
    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI
    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI    EcoNI EcoO109I     FalI     FseI     FspI
    FspAI    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SphI     SrfI     StuI     SwaI    TaqII     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI