(Linear) (Six-base) MAPSORT of: KIEE0831.seq  Check: 5290  from: 1  to: 756



KIEE0831.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AatII G_ACGT'C

Cuts at:      0     154     756 
   Size:        154     602
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     523     756 
   Size:        523     233
 
BanI G'GyrC_C

Cuts at:      0     524     756 
   Size:        524     232
 
BbeI G_GCGC'C

Cuts at:      0     528     756 
   Size:        528     228
 
BbvCI CC'TCA_GC

Cuts at:      0      13     756 
   Size:         13     743
 
BmgBI CAC'GTC

Cuts at:      0      88     756 
   Size:         88     668
 
Bpu10I CC'TnA_GC

Cuts at:      0      13     756 
   Size:         13     743
 
BsaBI GATnn'nnATC

Cuts at:      0     645     756 
   Size:        645     111
 
BsaHI Gr'CG_yC

Cuts at:      0     151     525     756 
   Size:        151     374     231

  Fragments arranged by size: 

                 374     231     151
 
BseYI C'CCAG_C

Cuts at:      0     122     756 
   Size:        122     634
 
BsmI GAATG_Cn'

Cuts at:      0     163     294     756 
   Size:        163     131     462

  Fragments arranged by size: 

                 462     163     131
 
BstYI r'GATC_y

Cuts at:      0     436     756 
   Size:        436     320
 
BtgI C'CryG_G

Cuts at:      0     176     756 
   Size:        176     580
 
EaeI y'GGCC_r

Cuts at:      0     197     756 
   Size:        197     559
 
EarI CTCTTCn'nnn_

Cuts at:      0     435     756 
   Size:        435     321
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     504     756 
   Size:        504     252
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     523     756 
   Size:        523     233
 
EcoRV GAT'ATC

Cuts at:      0     647     756 
   Size:        647     109
 
HaeII r_GCGC'y

Cuts at:      0     528     756 
   Size:        528     228
 
KasI G'GCGC_C

Cuts at:      0     524     756 
   Size:        524     232
 
MscI TGG'CCA

Cuts at:      0     199     756 
   Size:        199     557
 
MslI CAynn'nnrTG

Cuts at:      0     601     756 
   Size:        601     155
 
NarI GG'CG_CC

Cuts at:      0     525     756 
   Size:        525     231
 
NcoI C'CATG_G

Cuts at:      0     176     756 
   Size:        176     580
 
SfcI C'TryA_G

Cuts at:      0     134     693     756 
   Size:        134     559      63

  Fragments arranged by size: 

                 559     134      63
 
SfoI GGC'GCC

Cuts at:      0     526     756 
   Size:        526     230
 
SpeI A'CTAG_T

Cuts at:      0     304     756 
   Size:        304     452
 
StuI AGG'CCT

Cuts at:      0     535     756 
   Size:        535     221
 
StyI C'CwwG_G

Cuts at:      0     176     756 
   Size:        176     580
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     241     265     756 
   Size:        241      24     491

  Fragments arranged by size: 

                 491     241      24
 
XmnI GAAnn'nnTTC

Cuts at:      0     750     756 
   Size:        750       6
 
ZraI GAC'GTC

Cuts at:      0     152     756 
   Size:        152     604
 

 Enzymes that do cut:

    AatII     AcuI     BanI     BbeI    BbvCI    BmgBI   Bpu10I    BsaBI
    BsaHI    BseYI     BsmI    BstYI     BtgI     EaeI     EarI     EciI
  Eco57MI    EcoRV    HaeII     KasI     MscI     MslI     NarI     NcoI
     SfcI     SfoI     SpeI     StuI     StyI    TaqII     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
     BbsI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI
    BsaWI    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
  EcoICRI    EcoNI EcoO109I    EcoRI     FalI     FseI     FspI    FspAI
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
   MspA1I     NaeI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SphI     SrfI     SspI     SwaI     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI