(Linear) (Six-base) MAPSORT of: KIEE0829.seq  Check: 9484  from: 1  to: 828



KIEE0829.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:27  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     550     828 
   Size:        550     278
 
AleI CACnn'nnGTG

Cuts at:      0      43     828 
   Size:         43     785
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     424     828 
   Size:        424     404
 
AvrII C'CTAG_G

Cuts at:      0      63     828 
   Size:         63     765
 
BanI G'GyrC_C

Cuts at:      0       3     828 
   Size:          3     825
 
BanII G_rGCy'C

Cuts at:      0     649     828 
   Size:        649     179
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     102     295     828 
   Size:        102     193     533

  Fragments arranged by size: 

                 533     193     102
 
BfrBI ATG'CAT

Cuts at:      0     801     828 
   Size:        801      27
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     479     828 
   Size:        479     349
 
Bsp1286I G_dGCh'C

Cuts at:      0     649     828 
   Size:        649     179
 
BsrGI T'GTAC_A

Cuts at:      0     794     828 
   Size:        794      34
 
BstYI r'GATC_y

Cuts at:      0      13     828 
   Size:         13     815
 
BtgI C'CryG_G

Cuts at:      0      17     828 
   Size:         17     811
 
EaeI y'GGCC_r

Cuts at:      0      47     392     828 
   Size:         47     345     436

  Fragments arranged by size: 

                 436     345      47
 
EarI CTCTTCn'nnn_

Cuts at:      0     775     828 
   Size:        775      53
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     479     550     828 
   Size:        479      71     278

  Fragments arranged by size: 

                 479     278      71
 
EcoO109I rG'GnC_Cy

Cuts at:      0     332     828 
   Size:        332     496
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     424     614     828 
   Size:        424     190     214

  Fragments arranged by size: 

                 424     214     190
 
MscI TGG'CCA

Cuts at:      0      49     394     828 
   Size:         49     345     434

  Fragments arranged by size: 

                 434     345      49
 
MslI CAynn'nnrTG

Cuts at:      0      22      43     193     828 
   Size:         22      21     150     635

  Fragments arranged by size: 

                 635     150      22      21
 
MspA1I CmG'CkG

Cuts at:      0     760     828 
   Size:        760      68
 
NcoI C'CATG_G

Cuts at:      0      17     828 
   Size:         17     811
 
NsiI A_TGCA'T

Cuts at:      0     803     828 
   Size:        803      25
 
NspI r_CATG'y

Cuts at:      0     801     828 
   Size:        801      27
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      25      57     828 
   Size:         25      32     771

  Fragments arranged by size: 

                 771      32      25
 
PstI C_TGCA'G

Cuts at:      0      92     413     828 
   Size:         92     321     415

  Fragments arranged by size: 

                 415     321      92
 
PvuII CAG'CTG

Cuts at:      0     760     828 
   Size:        760      68
 
ScaI AGT'ACT

Cuts at:      0     415     828 
   Size:        415     413
 
SfcI C'TryA_G

Cuts at:      0      88     409     828 
   Size:         88     321     419

  Fragments arranged by size: 

                 419     321      88
 
StuI AGG'CCT

Cuts at:      0     730     828 
   Size:        730      98
 
StyI C'CwwG_G

Cuts at:      0      17      63     256     725     828 
   Size:         17      46     193     469     103

  Fragments arranged by size: 

                 469     193     103      46      17
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     207     828 
   Size:        207     621
 
TatI w'GTAC_w

Cuts at:      0     197     413     794     828 
   Size:        197     216     381      34

  Fragments arranged by size: 

                 381     216     197      34
 

 Enzymes that do cut:

     AcuI     AleI     AlfI    AvrII     BanI    BanII     BbsI    BfrBI
     BpmI Bsp1286I    BsrGI    BstYI     BtgI     EaeI     EarI  Eco57MI
 EcoO109I     MmeI     MscI     MslI   MspA1I     NcoI     NsiI     NspI
     PpiI     PstI    PvuII     ScaI     SfcI     StuI     StyI    TaqII
     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI     BaeI    BamHI     BbeI    BbvCI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI