(Linear) (Six-base) MAPSORT of: KIEE0828.seq  Check: 7085  from: 1  to: 570



KIEE0828.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:27  ..

ApoI r'AATT_y

Cuts at:      0     532     570 
   Size:        532      38
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0      16      49     570 
   Size:         16      33     521

  Fragments arranged by size: 

                 521      33      16
 
BanI G'GyrC_C

Cuts at:      0     103     570 
   Size:        103     467
 
BfrBI ATG'CAT

Cuts at:      0     125     570 
   Size:        125     445
 
Bme1580I G_kGCm'C

Cuts at:      0     269     570 
   Size:        269     301
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      48     178     291     570 
   Size:         48     130     113     279

  Fragments arranged by size: 

                 279     130     113      48
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     325     355     570 
   Size:        325      30     215

  Fragments arranged by size: 

                 325     215      30
 
BseYI C'CCAG_C

Cuts at:      0     433     570 
   Size:        433     137
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     189     570 
   Size:        189     381
 
BsiHKAI G_wGCw'C

Cuts at:      0      64     570 
   Size:         64     506
 
Bsp1286I G_dGCh'C

Cuts at:      0      64     269     570 
   Size:         64     205     301

  Fragments arranged by size: 

                 301     205      64
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     329     570 
   Size:        329     241
 
BsrDI GCAATG_nn'

Cuts at:      0      38     570 
   Size:         38     532
 
Bsu36I CC'TnA_GG

Cuts at:      0     514     570 
   Size:        514      56
 
BtgI C'CryG_G

Cuts at:      0     107     440     570 
   Size:        107     333     130

  Fragments arranged by size: 

                 333     130     107
 
ClaI AT'CG_AT

Cuts at:      0     161     570 
   Size:        161     409
 
EaeI y'GGCC_r

Cuts at:      0      11     570 
   Size:         11     559
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      48     178     291     570 
   Size:         48     130     113     279

  Fragments arranged by size: 

                 279     130     113      48
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     325     357     570 
   Size:        325      32     213

  Fragments arranged by size: 

                 325     213      32
 
HindIII A'AGCT_T

Cuts at:      0     347     570 
   Size:        347     223
 
MscI TGG'CCA

Cuts at:      0      13     570 
   Size:         13     557
 
MslI CAynn'nnrTG

Cuts at:      0      43     262     570 
   Size:         43     219     308

  Fragments arranged by size: 

                 308     219      43
 
NcoI C'CATG_G

Cuts at:      0     107     440     570 
   Size:        107     333     130

  Fragments arranged by size: 

                 333     130     107
 
NsiI A_TGCA'T

Cuts at:      0     127     570 
   Size:        127     443
 
NspI r_CATG'y

Cuts at:      0     129     570 
   Size:        129     441
 
SfcI C'TryA_G

Cuts at:      0      53     507     570 
   Size:         53     454      63

  Fragments arranged by size: 

                 454      63      53
 
SphI G_CATG'C

Cuts at:      0     129     570 
   Size:        129     441
 
StyI C'CwwG_G

Cuts at:      0     107     329     440     570 
   Size:        107     222     111     130

  Fragments arranged by size: 

                 222     130     111     107
 

 Enzymes that do cut:

     ApoI     BaeI     BanI    BfrBI Bme1580I     BpmI    BsaXI    BseYI
     BsgI  BsiHKAI Bsp1286I    BspMI    BsrDI   Bsu36I     BtgI     ClaI
     EaeI  Eco57MI    Hin4I  HindIII     MscI     MslI     NcoI     NsiI
     NspI     SfcI     SphI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII    BamHI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI     BglI    BglII
     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I    BtgZI     BtsI
    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SrfI     SspI     StuI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI