(Linear) (Six-base) MAPSORT of: KIEE0826.seq  Check: 9149  from: 1  to: 753



KIEE0826.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     691     707     753 
   Size:        691      16      46

  Fragments arranged by size: 

                 691      46      16
 
Bme1580I G_kGCm'C

Cuts at:      0     623     753 
   Size:        623     130
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     129     753 
   Size:        129     624
 
BsaBI GATnn'nnATC

Cuts at:      0     238     631     753 
   Size:        238     393     122

  Fragments arranged by size: 

                 393     238     122
 
BseYI C'CCAG_C

Cuts at:      0      36     753 
   Size:         36     717
 
Bsp1286I G_dGCh'C

Cuts at:      0     623     753 
   Size:        623     130
 
BspHI T'CATG_A

Cuts at:      0     398     753 
   Size:        398     355
 
BstYI r'GATC_y

Cuts at:      0     674     753 
   Size:        674      79
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     508     543     753 
   Size:        508      35     210

  Fragments arranged by size: 

                 508     210      35
 
EaeI y'GGCC_r

Cuts at:      0       2     753 
   Size:          2     751
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     691     707     753 
   Size:        691      16      46

  Fragments arranged by size: 

                 691      46      16
 
HincII GTy'rAC

Cuts at:      0     516     753 
   Size:        516     237
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     530     753 
   Size:        530     223
 
MscI TGG'CCA

Cuts at:      0       4     753 
   Size:          4     749
 
MslI CAynn'nnrTG

Cuts at:      0     166     292     753 
   Size:        166     126     461

  Fragments arranged by size: 

                 461     166     126
 
MspA1I CmG'CkG

Cuts at:      0     685     753 
   Size:        685      68
 
PvuII CAG'CTG

Cuts at:      0     685     753 
   Size:        685      68
 
SmlI C'TyrA_G

Cuts at:      0     108     753 
   Size:        108     645
 
StyI C'CwwG_G

Cuts at:      0      51     753 
   Size:         51     702
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     180     304     753 
   Size:        180     124     449

  Fragments arranged by size: 

                 449     180     124
 
TatI w'GTAC_w

Cuts at:      0     170     753 
   Size:        170     583
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     399     431     530     562     753 
   Size:        399      32      99      32     191

  Fragments arranged by size: 

                 399     191      99      32      32
 

 Enzymes that do cut:

     AcuI Bme1580I    BpuEI    BsaBI    BseYI Bsp1286I    BspHI    BstYI
    CspCI     EaeI  Eco57MI   HincII     MmeI     MscI     MslI   MspA1I
    PvuII     SmlI     StyI    TaqII     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI