Restriction map for
pF1KE0824
(Linear) (Six-base) MAPSORT of: KIEE0824.seq Check: 7991 from: 1 to: 804
KIEE0824.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AflIII A'CryG_T
Cuts at: 0 80 804
Size: 80 724
AleI CACnn'nnGTG
Cuts at: 0 605 804
Size: 605 199
ApaI G_GGCC'C
Cuts at: 0 383 804
Size: 383 421
ApaLI G'TGCA_C
Cuts at: 0 291 804
Size: 291 513
ApoI r'AATT_y
Cuts at: 0 410 804
Size: 410 394
AvaI C'yCGr_G
Cuts at: 0 383 804
Size: 383 421
BanI G'GyrC_C
Cuts at: 0 766 804
Size: 766 38
BanII G_rGCy'C
Cuts at: 0 383 804
Size: 383 421
BbeI G_GCGC'C
Cuts at: 0 770 804
Size: 770 34
BfrBI ATG'CAT
Cuts at: 0 161 804
Size: 161 643
Bme1580I G_kGCm'C
Cuts at: 0 295 383 804
Size: 295 88 421
Fragments arranged by size:
421 295 88
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 770 804
Size: 770 34
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 303 804
Size: 244 59 501
Fragments arranged by size:
501 244 59
Bpu10I CC'TnA_GC
Cuts at: 0 755 804
Size: 755 49
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 804
Size: 409 395
BsaHI Gr'CG_yC
Cuts at: 0 767 804
Size: 767 37
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 585 688 804
Size: 585 103 116
Fragments arranged by size:
585 116 103
BsiHKAI G_wGCw'C
Cuts at: 0 295 783 804
Size: 295 488 21
Fragments arranged by size:
488 295 21
BsmI GAATG_Cn'
Cuts at: 0 756 804
Size: 756 48
Bsp1286I G_dGCh'C
Cuts at: 0 295 383 783 804
Size: 295 88 400 21
Fragments arranged by size:
400 295 88 21
BspHI T'CATG_A
Cuts at: 0 275 804
Size: 275 529
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 598 804
Size: 598 206
BstXI CCAn_nnnn'nTGG
Cuts at: 0 114 804
Size: 114 690
BstYI r'GATC_y
Cuts at: 0 551 804
Size: 551 253
BtgI C'CryG_G
Cuts at: 0 635 804
Size: 635 169
BtsI GCAGTG_nn'
Cuts at: 0 781 804
Size: 781 23
DrdI GACnn_nn'nnGTC
Cuts at: 0 17 804
Size: 17 787
EaeI y'GGCC_r
Cuts at: 0 68 242 804
Size: 68 174 562
Fragments arranged by size:
562 174 68
EarI CTCTTCn'nnn_
Cuts at: 0 406 625 804
Size: 406 219 179
Fragments arranged by size:
406 219 179
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 303 804
Size: 244 59 501
Fragments arranged by size:
501 244 59
EcoNI CCTnn'n_nnAGG
Cuts at: 0 231 804
Size: 231 573
EcoO109I rG'GnC_Cy
Cuts at: 0 380 804
Size: 380 424
HaeII r_GCGC'y
Cuts at: 0 770 804
Size: 770 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 288 320 726 758 804
Size: 288 32 406 32 46
Fragments arranged by size:
406 288 46 32 32
HincII GTy'rAC
Cuts at: 0 453 804
Size: 453 351
HpaI GTT'AAC
Cuts at: 0 453 804
Size: 453 351
KasI G'GCGC_C
Cuts at: 0 766 804
Size: 766 38
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 474 594 804
Size: 119 355 120 210
Fragments arranged by size:
355 210 120 119
MscI TGG'CCA
Cuts at: 0 70 244 804
Size: 70 174 560
Fragments arranged by size:
560 174 70
MslI CAynn'nnrTG
Cuts at: 0 79 605 634 804
Size: 79 526 29 170
Fragments arranged by size:
526 170 79 29
NarI GG'CG_CC
Cuts at: 0 767 804
Size: 767 37
NcoI C'CATG_G
Cuts at: 0 635 804
Size: 635 169
NsiI A_TGCA'T
Cuts at: 0 163 804
Size: 163 641
NspI r_CATG'y
Cuts at: 0 84 349 528 804
Size: 84 265 179 276
Fragments arranged by size:
276 265 179 84
PciI A'CATG_T
Cuts at: 0 80 804
Size: 80 724
PspOMI G'GGCC_C
Cuts at: 0 379 804
Size: 379 425
PstI C_TGCA'G
Cuts at: 0 788 804
Size: 788 16
SfcI C'TryA_G
Cuts at: 0 197 738 784 804
Size: 197 541 46 20
Fragments arranged by size:
541 197 46 20
SfoI GGC'GCC
Cuts at: 0 768 804
Size: 768 36
SmaI CCC'GGG
Cuts at: 0 385 804
Size: 385 419
SmlI C'TyrA_G
Cuts at: 0 424 804
Size: 424 380
StuI AGG'CCT
Cuts at: 0 580 804
Size: 580 224
StyI C'CwwG_G
Cuts at: 0 635 804
Size: 635 169
TatI w'GTAC_w
Cuts at: 0 47 263 804
Size: 47 216 541
Fragments arranged by size:
541 216 47
Tth111I GACn'n_nGTC
Cuts at: 0 795 804
Size: 795 9
XmaI C'CCGG_G
Cuts at: 0 383 804
Size: 383 421
Enzymes that do cut:
AflIII AleI ApaI ApaLI ApoI AvaI BanI BanII
BbeI BfrBI Bme1580I BplI BpmI Bpu10I BpuEI BsaHI
BseRI BsiHKAI BsmI Bsp1286I BspHI BspMI BstXI BstYI
BtgI BtsI DrdI EaeI EarI Eco57MI EcoNI EcoO109I
HaeII Hin4I HincII HpaI KasI MmeI MscI MslI
NarI NcoI NsiI NspI PciI PspOMI PstI SfcI
SfoI SmaI SmlI StuI StyI TatI Tth111I XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AlfI AloI AlwNI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI BmgBI BmrI BmtI BsaI BsaAI
BsaBI BsaWI BsaXI BseYI BsgI BsiEI BsiWI BsmBI
BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII EagI EciI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI HindIII KpnI MfeI MluI MspA1I NaeI
NdeI NgoMIV NheI NotI NruI PacI PasI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI TaqII TstI XbaI
XcmI XhoI XmnI ZraI