(Linear) (Six-base) MAPSORT of: KIEE0820.seq  Check: 4942  from: 1  to: 582



KIEE0820.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AatII G_ACGT'C

Cuts at:      0     167     582 
   Size:        167     415
 
ApoI r'AATT_y

Cuts at:      0     447     582 
   Size:        447     135
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     329     582 
   Size:        329     253
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     281     582 
   Size:        281     301
 
BsaAI yAC'GTr

Cuts at:      0      18     582 
   Size:         18     564
 
BsaHI Gr'CG_yC

Cuts at:      0     164     582 
   Size:        164     418
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     372     582 
   Size:        372     210
 
BsiEI CG_ry'CG

Cuts at:      0      12     582 
   Size:         12     570
 
BspHI T'CATG_A

Cuts at:      0     245     582 
   Size:        245     337
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     434     582 
   Size:        434     148
 
EaeI y'GGCC_r

Cuts at:      0      20     582 
   Size:         20     562
 
EcoO109I rG'GnC_Cy

Cuts at:      0     275     582 
   Size:        275     307
 
EcoRI G'AATT_C

Cuts at:      0     447     582 
   Size:        447     135
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     185     582 
   Size:        185     397
 
MscI TGG'CCA

Cuts at:      0      22     582 
   Size:         22     560
 
MspA1I CmG'CkG

Cuts at:      0      63     144     582 
   Size:         63      81     438

  Fragments arranged by size: 

                 438      81      63
 
PpuMI rG'GwC_Cy

Cuts at:      0     275     582 
   Size:        275     307
 
PsiI TTA'TAA

Cuts at:      0     325     582 
   Size:        325     257
 
PstI C_TGCA'G

Cuts at:      0      73     261     431     582 
   Size:         73     188     170     151

  Fragments arranged by size: 

                 188     170     151      73
 
PvuII CAG'CTG

Cuts at:      0     144     582 
   Size:        144     438
 
SanDI GG'GwC_CC

Cuts at:      0     275     582 
   Size:        275     307
 
SfcI C'TryA_G

Cuts at:      0      69     257     427     582 
   Size:         69     188     170     155

  Fragments arranged by size: 

                 188     170     155      69
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     238     582 
   Size:        238     344
 
ZraI GAC'GTC

Cuts at:      0     165     582 
   Size:        165     417
 

 Enzymes that do cut:

    AatII     ApoI     BbsI     BmrI    BsaAI    BsaHI     BsgI    BsiEI
    BspHI    BspMI     EaeI EcoO109I    EcoRI     MmeI     MscI   MspA1I
    PpuMI     PsiI     PstI    PvuII    SanDI     SfcI    TaqII     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaBI    BsaWI    BsaXI    BseRI    BseYI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI