(Linear) (Six-base) MAPSORT of: KIEE0817.seq  Check: 856  from: 1  to: 762



KIEE0817.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AatII G_ACGT'C

Cuts at:      0     221     762 
   Size:        221     541
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      42     360     762 
   Size:         42     318     402

  Fragments arranged by size: 

                 402     318      42
 
AflIII A'CryG_T

Cuts at:      0     413     762 
   Size:        413     349
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     684     762 
   Size:        684      78
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     300     762 
   Size:        300     462
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     681     762 
   Size:        681      81
 
BsaBI GATnn'nnATC

Cuts at:      0     148     551     762 
   Size:        148     403     211

  Fragments arranged by size: 

                 403     211     148
 
BsaHI Gr'CG_yC

Cuts at:      0     218     423     762 
   Size:        218     205     339

  Fragments arranged by size: 

                 339     218     205
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      76     762 
   Size:         76     686
 
BseYI C'CCAG_C

Cuts at:      0     167     464     762 
   Size:        167     297     298

  Fragments arranged by size: 

                 298     297     167
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     414     762 
   Size:        414     348
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     225     762 
   Size:        225     537
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     283     762 
   Size:        283     479
 
BsrDI GCAATG_nn'

Cuts at:      0     527     762 
   Size:        527     235
 
BstYI r'GATC_y

Cuts at:      0     689     762 
   Size:        689      73
 
BtgI C'CryG_G

Cuts at:      0     251     359     762 
   Size:        251     108     403

  Fragments arranged by size: 

                 403     251     108
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     219     254     762 
   Size:        219      35     508

  Fragments arranged by size: 

                 508     219      35
 
EarI CTCTTCn'nnn_

Cuts at:      0      94     401     762 
   Size:         94     307     361

  Fragments arranged by size: 

                 361     307      94
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      55     762 
   Size:         55     707
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      42     360     681     762 
   Size:         42     318     321      81

  Fragments arranged by size: 

                 321     318      81      42
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     735     762 
   Size:        735      27
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     588     620     762 
   Size:        588      32     142

  Fragments arranged by size: 

                 588     142      32
 
MfeI C'AATT_G

Cuts at:      0     638     762 
   Size:        638     124
 
MslI CAynn'nnrTG

Cuts at:      0      29     139     181     743     762 
   Size:         29     110      42     562      19

  Fragments arranged by size: 

                 562     110      42      29      19
 
MspA1I CmG'CkG

Cuts at:      0     171     762 
   Size:        171     591
 
NcoI C'CATG_G

Cuts at:      0     251     359     762 
   Size:        251     108     403

  Fragments arranged by size: 

                 403     251     108
 
NspI r_CATG'y

Cuts at:      0     417     762 
   Size:        417     345
 
PciI A'CATG_T

Cuts at:      0     413     762 
   Size:        413     349
 
PvuII CAG'CTG

Cuts at:      0     171     762 
   Size:        171     591
 
SfcI C'TryA_G

Cuts at:      0     111     762 
   Size:        111     651
 
StyI C'CwwG_G

Cuts at:      0     251     359     762 
   Size:        251     108     403

  Fragments arranged by size: 

                 403     251     108
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     733     762 
   Size:        733      29
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     123     155     762 
   Size:        123      32     607

  Fragments arranged by size: 

                 607     123      32
 
ZraI GAC'GTC

Cuts at:      0     219     762 
   Size:        219     543
 

 Enzymes that do cut:

    AatII     AcuI   AflIII    BciVI     BmrI     BpmI    BsaBI    BsaHI
    BseRI    BseYI     BsgI    BsmBI    BspMI    BsrDI    BstYI     BtgI
    CspCI     EarI     EciI  Eco57MI     FalI    Hin4I     MfeI     MslI
   MspA1I     NcoI     NspI     PciI    PvuII     SfcI     StyI    TaqII
     TstI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaWI    BsaXI    BsiEI  BsiHKAI    BsiWI     BsmI Bsp1286I
    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
     DraI   DraIII     DrdI     EaeI     EagI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MluI     MmeI     MscI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     TatI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI