Restriction map for
pF1KE0816
(Linear) (Six-base) MAPSORT of: KIEE0816.seq Check: 4725 from: 1 to: 678
KIEE0816.orf
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AccI GT'mk_AC
Cuts at: 0 101 542 678
Size: 101 441 136
Fragments arranged by size:
441 136 101
Acc65I G'GTAC_C
Cuts at: 0 365 678
Size: 365 313
AleI CACnn'nnGTG
Cuts at: 0 631 678
Size: 631 47
ApoI r'AATT_y
Cuts at: 0 65 249 678
Size: 65 184 429
Fragments arranged by size:
429 184 65
BanI G'GyrC_C
Cuts at: 0 365 678
Size: 365 313
BanII G_rGCy'C
Cuts at: 0 610 678
Size: 610 68
BclI T'GATC_A
Cuts at: 0 522 678
Size: 522 156
BglII A'GATC_T
Cuts at: 0 209 678
Size: 209 469
Bme1580I G_kGCm'C
Cuts at: 0 325 678
Size: 325 353
BmrI ACTGGGnnnn_n'
Cuts at: 0 128 678
Size: 128 550
BsaAI yAC'GTr
Cuts at: 0 180 678
Size: 180 498
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 382 412 678
Size: 382 30 266
Fragments arranged by size:
382 266 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 161 210 373 678
Size: 161 49 163 305
Fragments arranged by size:
305 163 161 49
BsiHKAI G_wGCw'C
Cuts at: 0 185 610 665 678
Size: 185 425 55 13
Fragments arranged by size:
425 185 55 13
BsmI GAATG_Cn'
Cuts at: 0 337 678
Size: 337 341
Bsp1286I G_dGCh'C
Cuts at: 0 185 325 610 665 678
Size: 185 140 285 55 13
Fragments arranged by size:
285 185 140 55 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 232 678
Size: 232 446
BsrGI T'GTAC_A
Cuts at: 0 635 678
Size: 635 43
BstXI CCAn_nnnn'nTGG
Cuts at: 0 243 421 678
Size: 243 178 257
Fragments arranged by size:
257 243 178
BstYI r'GATC_y
Cuts at: 0 209 678
Size: 209 469
BtgI C'CryG_G
Cuts at: 0 629 678
Size: 629 49
BtsI GCAGTG_nn'
Cuts at: 0 149 385 678
Size: 149 236 293
Fragments arranged by size:
293 236 149
EaeI y'GGCC_r
Cuts at: 0 128 371 411 678
Size: 128 243 40 267
Fragments arranged by size:
267 243 128 40
EcoICRI GAG'CTC
Cuts at: 0 608 678
Size: 608 70
EcoO109I rG'GnC_Cy
Cuts at: 0 647 678
Size: 647 31
EcoRV GAT'ATC
Cuts at: 0 17 678
Size: 17 661
KpnI G_GTAC'C
Cuts at: 0 369 678
Size: 369 309
MscI TGG'CCA
Cuts at: 0 130 373 413 678
Size: 130 243 40 265
Fragments arranged by size:
265 243 130 40
MslI CAynn'nnrTG
Cuts at: 0 430 631 678
Size: 430 201 47
Fragments arranged by size:
430 201 47
NcoI C'CATG_G
Cuts at: 0 629 678
Size: 629 49
NspI r_CATG'y
Cuts at: 0 243 592 678
Size: 243 349 86
Fragments arranged by size:
349 243 86
PasI CC'CwG_GG
Cuts at: 0 318 678
Size: 318 360
PpuMI rG'GwC_Cy
Cuts at: 0 647 678
Size: 647 31
SacI G_AGCT'C
Cuts at: 0 610 678
Size: 610 68
StyI C'CwwG_G
Cuts at: 0 459 629 642 656 678
Size: 459 170 13 14 22
Fragments arranged by size:
459 170 22 14 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 398 678
Size: 398 280
TatI w'GTAC_w
Cuts at: 0 635 678
Size: 635 43
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 382 414 678
Size: 382 32 264
Fragments arranged by size:
382 264 32
Enzymes that do cut:
AccI Acc65I AleI ApoI BanI BanII BclI BglII
Bme1580I BmrI BsaAI BsaXI BsgI BsiHKAI BsmI Bsp1286I
BspMI BsrGI BstXI BstYI BtgI BtsI EaeI EcoICRI
EcoO109I EcoRV KpnI MscI MslI NcoI NspI PasI
PpuMI SacI StyI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII AclI AcuI AfeI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BbvCI
BcgI BciVI BfrBI BglI BlpI BmgBI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EarI
EciI Eco57MI EcoNI EcoRI FalI FseI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI MfeI MluI
MmeI MspA1I NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI