(Linear) (Six-base) MAPSORT of: KIEE0815.seq  Check: 8250  from: 1  to: 657



KIEE0815.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:27  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     407     491     657 
   Size:        407      84     166

  Fragments arranged by size: 

                 407     166      84
 
AlwNI CAG_nnn'CTG

Cuts at:      0     514     657 
   Size:        514     143
 
ApoI r'AATT_y

Cuts at:      0      18     657 
   Size:         18     639
 
BanI G'GyrC_C

Cuts at:      0     445     657 
   Size:        445     212
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     395     426     657 
   Size:        395      31     231

  Fragments arranged by size: 

                 395     231      31
 
BfrBI ATG'CAT

Cuts at:      0     218     657 
   Size:        218     439
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     613     657 
   Size:        613      44
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      61      91     657 
   Size:         61      30     566

  Fragments arranged by size: 

                 566      61      30
 
BseYI C'CCAG_C

Cuts at:      0     404     657 
   Size:        404     253
 
BspHI T'CATG_A

Cuts at:      0     101     221     657 
   Size:        101     120     436

  Fragments arranged by size: 

                 436     120     101
 
BsrGI T'GTAC_A

Cuts at:      0       2     657 
   Size:          2     655
 
EaeI y'GGCC_r

Cuts at:      0      65     657 
   Size:         65     592
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     407     491     657 
   Size:        407      84     166

  Fragments arranged by size: 

                 407     166      84
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     177     657 
   Size:        177     480
 
HindIII A'AGCT_T

Cuts at:      0     527     657 
   Size:        527     130
 
MscI TGG'CCA

Cuts at:      0      67     657 
   Size:         67     590
 
MslI CAynn'nnrTG

Cuts at:      0     264     589     657 
   Size:        264     325      68

  Fragments arranged by size: 

                 325     264      68
 
NsiI A_TGCA'T

Cuts at:      0     220     657 
   Size:        220     437
 
PfoI T'CCnGG_A

Cuts at:      0      29     603     657 
   Size:         29     574      54

  Fragments arranged by size: 

                 574      54      29
 
SspI AAT'ATT

Cuts at:      0     333     657 
   Size:        333     324
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     418     632     657 
   Size:        418     214      25

  Fragments arranged by size: 

                 418     214      25
 
TatI w'GTAC_w

Cuts at:      0       2     657 
   Size:          2     655
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI     BanI     BbsI    BfrBI     BmrI    BsaXI
    BseYI    BspHI    BsrGI     EaeI  Eco57MI    EcoNI  HindIII     MscI
     MslI     NsiI     PfoI     SspI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EarI     EciI  EcoICRI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NspI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     StuI     StyI     SwaI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI