(Linear) (Six-base) MAPSORT of: KIEE0814.seq  Check: 4384  from: 1  to: 768



KIEE0814.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:18  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     768 
   Size:        169     599
 
AseI AT'TA_AT

Cuts at:      0      34     768 
   Size:         34     734
 
BamHI G'GATC_C

Cuts at:      0     761     768 
   Size:        761       7
 
BbvCI CC'TCA_GC

Cuts at:      0     268     768 
   Size:        268     500
 
BglI GCCn_nnn'nGGC

Cuts at:      0     433     768 
   Size:        433     335
 
Bpu10I CC'TnA_GC

Cuts at:      0     268     768 
   Size:        268     500
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     397     768 
   Size:        397     371
 
BsiEI CG_ry'CG

Cuts at:      0     444     768 
   Size:        444     324
 
BsiHKAI G_wGCw'C

Cuts at:      0     512     768 
   Size:        512     256
 
BsmI GAATG_Cn'

Cuts at:      0     218     326     768 
   Size:        218     108     442

  Fragments arranged by size: 

                 442     218     108
 
Bsp1286I G_dGCh'C

Cuts at:      0     512     768 
   Size:        512     256
 
BsrBI CCG'CTC

Cuts at:      0     345     768 
   Size:        345     423
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     195     768 
   Size:        195     573
 
BstYI r'GATC_y

Cuts at:      0     761     768 
   Size:        761       7
 
Bsu36I CC'TnA_GG

Cuts at:      0     757     768 
   Size:        757      11
 
BtgI C'CryG_G

Cuts at:      0      80     768 
   Size:         80     688
 
EaeI y'GGCC_r

Cuts at:      0     425     452     768 
   Size:        425      27     316

  Fragments arranged by size: 

                 425     316      27
 
EarI CTCTTCn'nnn_

Cuts at:      0     166     768 
   Size:        166     602
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     768 
   Size:        169     599
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     524     556     768 
   Size:        524      32     212

  Fragments arranged by size: 

                 524     212      32
 
MfeI C'AATT_G

Cuts at:      0     560     768 
   Size:        560     208
 
MscI TGG'CCA

Cuts at:      0     427     454     768 
   Size:        427      27     314

  Fragments arranged by size: 

                 427     314      27
 
MslI CAynn'nnrTG

Cuts at:      0     253     768 
   Size:        253     515
 
MspA1I CmG'CkG

Cuts at:      0     272     768 
   Size:        272     496
 
NcoI C'CATG_G

Cuts at:      0      80     768 
   Size:         80     688
 
NspI r_CATG'y

Cuts at:      0     371     768 
   Size:        371     397
 
PvuII CAG'CTG

Cuts at:      0     272     768 
   Size:        272     496
 
StyI C'CwwG_G

Cuts at:      0      80     768 
   Size:         80     688
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     267     330     768 
   Size:        267      63     438

  Fragments arranged by size: 

                 438     267      63
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     195     768 
   Size:        195     573
 
XmnI GAAnn'nnTTC

Cuts at:      0      35     768 
   Size:         35     733
 

 Enzymes that do cut:

     AcuI     AseI    BamHI    BbvCI     BglI   Bpu10I     BsgI    BsiEI
  BsiHKAI     BsmI Bsp1286I    BsrBI    BstXI    BstYI   Bsu36I     BtgI
     EaeI     EarI  Eco57MI    Hin4I     MfeI     MscI     MslI   MspA1I
     NcoI     NspI    PvuII     StyI    TaqII     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI    AsiSI     AvaI    AvrII     BaeI     BanI    BanII
     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiWI
    BsmBI    BspEI    BspHI    BspMI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII  BstZ17I    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MluI     MmeI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     ZraI