(Linear) (Six-base) MAPSORT of: KIEE0813.seq  Check: 7565  from: 1  to: 762



KIEE0813.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:17  ..

AccI GT'mk_AC

Cuts at:      0     220     762 
   Size:        220     542
 
AseI AT'TA_AT

Cuts at:      0     287     762 
   Size:        287     475
 
BanII G_rGCy'C

Cuts at:      0     284     440     762 
   Size:        284     156     322

  Fragments arranged by size: 

                 322     284     156
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     420     762 
   Size:        420     342
 
BbvCI CC'TCA_GC

Cuts at:      0     518     762 
   Size:        518     244
 
Bpu10I CC'TnA_GC

Cuts at:      0     518     762 
   Size:        518     244
 
BsaAI yAC'GTr

Cuts at:      0     195     762 
   Size:        195     567
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     362     762 
   Size:        362     400
 
Bsp1286I G_dGCh'C

Cuts at:      0     284     440     762 
   Size:        284     156     322

  Fragments arranged by size: 

                 322     284     156
 
BsrDI GCAATG_nn'

Cuts at:      0     440     452     491     762 
   Size:        440      12      39     271

  Fragments arranged by size: 

                 440     271      39      12
 
BstEII G'GTnAC_C

Cuts at:      0     567     762 
   Size:        567     195
 
BstZ17I GTA'TAC

Cuts at:      0     221     762 
   Size:        221     541
 
BtgI C'CryG_G

Cuts at:      0     563     762 
   Size:        563     199
 
EaeI y'GGCC_r

Cuts at:      0     197     762 
   Size:        197     565
 
EarI CTCTTCn'nnn_

Cuts at:      0     380     762 
   Size:        380     382
 
EcoRV GAT'ATC

Cuts at:      0      36     762 
   Size:         36     726
 
MscI TGG'CCA

Cuts at:      0     199     762 
   Size:        199     563
 
MslI CAynn'nnrTG

Cuts at:      0     407     445     469     762 
   Size:        407      38      24     293

  Fragments arranged by size: 

                 407     293      38      24
 
MspA1I CmG'CkG

Cuts at:      0     517     762 
   Size:        517     245
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     195     762 
   Size:        195     567
 
PvuII CAG'CTG

Cuts at:      0     517     762 
   Size:        517     245
 
SfcI C'TryA_G

Cuts at:      0     121     762 
   Size:        121     641
 
SspI AAT'ATT

Cuts at:      0     342     762 
   Size:        342     420
 
StyI C'CwwG_G

Cuts at:      0      12     422     626     762 
   Size:         12     410     204     136

  Fragments arranged by size: 

                 410     204     136      12
 
TatI w'GTAC_w

Cuts at:      0       2     100     762 
   Size:          2      98     662

  Fragments arranged by size: 

                 662      98       2
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     653     685     762 
   Size:        653      32      77

  Fragments arranged by size: 

                 653      77      32
 

 Enzymes that do cut:

     AccI     AseI    BanII     BbsI    BbvCI   Bpu10I    BsaAI    BseRI
 Bsp1286I    BsrDI   BstEII  BstZ17I     BtgI     EaeI     EarI    EcoRV
     MscI     MslI   MspA1I    PflMI    PvuII     SfcI     SspI     StyI
     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI    BstYI   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     StuI     SwaI    TaqII  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI