(Linear) (Six-base) MAPSORT of: KIEE0809.seq  Check: 6365  from: 1  to: 1005



KIEE0809.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:17  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     732    1005 
   Size:        732     273
 
Acc65I G'GTAC_C

Cuts at:      0     308     785    1005 
   Size:        308     477     220

  Fragments arranged by size: 

                 477     308     220
 
AleI CACnn'nnGTG

Cuts at:      0      70    1005 
   Size:         70     935
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     832    1005 
   Size:        832     173
 
AlwNI CAG_nnn'CTG

Cuts at:      0     122    1005 
   Size:        122     883
 
AvrII C'CTAG_G

Cuts at:      0     882    1005 
   Size:        882     123
 
BanI G'GyrC_C

Cuts at:      0     308     785    1005 
   Size:        308     477     220

  Fragments arranged by size: 

                 477     308     220
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     229     537    1005 
   Size:        229     308     468

  Fragments arranged by size: 

                 468     308     229
 
BclI T'GATC_A

Cuts at:      0     293    1005 
   Size:        293     712
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     719    1005 
   Size:        719     286
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     183     647     969    1005 
   Size:        183     464     322      36

  Fragments arranged by size: 

                 464     322     183      36
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     550    1005 
   Size:        550     455
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     181     211    1005 
   Size:        181      30     794

  Fragments arranged by size: 

                 794     181      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     526     904    1005 
   Size:        526     378     101

  Fragments arranged by size: 

                 526     378     101
 
BseYI C'CCAG_C

Cuts at:      0     525     652    1005 
   Size:        525     127     353

  Fragments arranged by size: 

                 525     353     127
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     252     483     760    1005 
   Size:        252     231     277     245

  Fragments arranged by size: 

                 277     252     245     231
 
BsiHKAI G_wGCw'C

Cuts at:      0     902    1005 
   Size:        902     103
 
Bsp1286I G_dGCh'C

Cuts at:      0     902    1005 
   Size:        902     103
 
BspHI T'CATG_A

Cuts at:      0     347    1005 
   Size:        347     658
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     732    1005 
   Size:        732     273
 
BsrDI GCAATG_nn'

Cuts at:      0      53     374    1005 
   Size:         53     321     631

  Fragments arranged by size: 

                 631     321      53
 
BstBI TT'CG_AA

Cuts at:      0     597    1005 
   Size:        597     408
 
BtgI C'CryG_G

Cuts at:      0     620     643    1005 
   Size:        620      23     362

  Fragments arranged by size: 

                 620     362      23
 
BtsI GCAGTG_nn'

Cuts at:      0     466    1005 
   Size:        466     539
 
ClaI AT'CG_AT

Cuts at:      0     636    1005 
   Size:        636     369
 
DraIII CAC_nnn'GTG

Cuts at:      0     495    1005 
   Size:        495     510
 
EaeI y'GGCC_r

Cuts at:      0     167     362     926    1005 
   Size:        167     195     564      79

  Fragments arranged by size: 

                 564     195     167      79
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     183     647     969    1005 
   Size:        183     464     322      36

  Fragments arranged by size: 

                 464     322     183      36
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     303    1005 
   Size:        303     702
 
EcoO109I rG'GnC_Cy

Cuts at:      0     368    1005 
   Size:        368     637
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     345    1005 
   Size:        345     660
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     181     213     851     883    1005 
   Size:        181      32     638      32     122

  Fragments arranged by size: 

                 638     181     122      32      32
 
KpnI G_GTAC'C

Cuts at:      0     312     789    1005 
   Size:        312     477     216

  Fragments arranged by size: 

                 477     312     216
 
MscI TGG'CCA

Cuts at:      0     169    1005 
   Size:        169     836
 
MslI CAynn'nnrTG

Cuts at:      0      70     104     286    1005 
   Size:         70      34     182     719

  Fragments arranged by size: 

                 719     182      70      34
 
MspA1I CmG'CkG

Cuts at:      0     268    1005 
   Size:        268     737
 
NcoI C'CATG_G

Cuts at:      0     620    1005 
   Size:        620     385
 
NspI r_CATG'y

Cuts at:      0     564     613    1005 
   Size:        564      49     392

  Fragments arranged by size: 

                 564     392      49
 
PasI CC'CwG_GG

Cuts at:      0     953    1005 
   Size:        953      52
 
PfoI T'CCnGG_A

Cuts at:      0     709    1005 
   Size:        709     296
 
PstI C_TGCA'G

Cuts at:      0     866    1005 
   Size:        866     139
 
PvuII CAG'CTG

Cuts at:      0     268    1005 
   Size:        268     737
 
SfcI C'TryA_G

Cuts at:      0     862    1005 
   Size:        862     143
 
SmlI C'TyrA_G

Cuts at:      0     565    1005 
   Size:        565     440
 
SpeI A'CTAG_T

Cuts at:      0      69    1005 
   Size:         69     936
 
StyI C'CwwG_G

Cuts at:      0     620     882    1005 
   Size:        620     262     123

  Fragments arranged by size: 

                 620     262     123
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     410     442    1005 
   Size:        410      32     563

  Fragments arranged by size: 

                 563     410      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     106    1005 
   Size:        106     899
 

 Enzymes that do cut:

     AarI   Acc65I     AleI     AlfI    AlwNI    AvrII     BanI     BbsI
     BclI     BplI     BpmI    BpuEI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI Bsp1286I    BspHI    BspMI    BsrDI    BstBI     BtgI     BtsI
     ClaI   DraIII     EaeI  Eco57MI    EcoNI EcoO109I     FalI    Hin4I
     KpnI     MscI     MslI   MspA1I     NcoI     NspI     PasI     PfoI
     PstI    PvuII     SfcI     SmlI     SpeI     StyI     TstI     XcmI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
     AvaI     BaeI    BamHI    BanII     BbeI    BbvCI     BcgI    BciVI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI    CspCI
     DraI     DrdI     EagI     EarI     EciI  EcoICRI    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     MfeI     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TatI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI