(Linear) (Six-base) MAPSORT of: KIEE0808.seq  Check: 1773  from: 1  to: 843



KIEE0808.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:17  ..

AccI GT'mk_AC

Cuts at:      0     386     843 
   Size:        386     457
 
AlwNI CAG_nnn'CTG

Cuts at:      0     136     235     843 
   Size:        136      99     608

  Fragments arranged by size: 

                 608     136      99
 
BanII G_rGCy'C

Cuts at:      0      74     843 
   Size:         74     769
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     575     790     843 
   Size:        575     215      53

  Fragments arranged by size: 

                 575     215      53
 
BbvCI CC'TCA_GC

Cuts at:      0     102     843 
   Size:        102     741
 
BclI T'GATC_A

Cuts at:      0      44     843 
   Size:         44     799
 
BglI GCCn_nnn'nGGC

Cuts at:      0     457     843 
   Size:        457     386
 
Bme1580I G_kGCm'C

Cuts at:      0     752     843 
   Size:        752      91
 
Bpu10I CC'TnA_GC

Cuts at:      0     102     843 
   Size:        102     741
 
BsaAI yAC'GTr

Cuts at:      0     617     843 
   Size:        617     226
 
BseYI C'CCAG_C

Cuts at:      0     657     843 
   Size:        657     186
 
BsiHKAI G_wGCw'C

Cuts at:      0     440     843 
   Size:        440     403
 
BsmI GAATG_Cn'

Cuts at:      0     661     843 
   Size:        661     182
 
Bsp1286I G_dGCh'C

Cuts at:      0      74     440     752     843 
   Size:         74     366     312      91

  Fragments arranged by size: 

                 366     312      91      74
 
BspHI T'CATG_A

Cuts at:      0      47     843 
   Size:         47     796
 
BsrDI GCAATG_nn'

Cuts at:      0     257     446     843 
   Size:        257     189     397

  Fragments arranged by size: 

                 397     257     189
 
BsrGI T'GTAC_A

Cuts at:      0       2     843 
   Size:          2     841
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     235     843 
   Size:        235     608
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     688     843 
   Size:        688     155
 
BstZ17I GTA'TAC

Cuts at:      0     387     843 
   Size:        387     456
 
Bsu36I CC'TnA_GG

Cuts at:      0     137     843 
   Size:        137     706
 
BtgI C'CryG_G

Cuts at:      0     167     843 
   Size:        167     676
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     667     843 
   Size:        667     176
 
BtsI GCAGTG_nn'

Cuts at:      0     575     843 
   Size:        575     268
 
DraIII CAC_nnn'GTG

Cuts at:      0     570     843 
   Size:        570     273
 
EaeI y'GGCC_r

Cuts at:      0     272     843 
   Size:        272     571
 
EarI CTCTTCn'nnn_

Cuts at:      0      88     843 
   Size:         88     755
 
EcoO109I rG'GnC_Cy

Cuts at:      0     606     843 
   Size:        606     237
 
MscI TGG'CCA

Cuts at:      0     274     843 
   Size:        274     569
 
MslI CAynn'nnrTG

Cuts at:      0     223     632     643     724     843 
   Size:        223     409      11      81     119

  Fragments arranged by size: 

                 409     223     119      81      11
 
MspA1I CmG'CkG

Cuts at:      0     124     340     843 
   Size:        124     216     503

  Fragments arranged by size: 

                 503     216     124
 
NcoI C'CATG_G

Cuts at:      0     167     843 
   Size:        167     676
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     168     843 
   Size:        168     675
 
PstI C_TGCA'G

Cuts at:      0     132     843 
   Size:        132     711
 
PvuII CAG'CTG

Cuts at:      0     340     843 
   Size:        340     503
 
SfcI C'TryA_G

Cuts at:      0     128     515     843 
   Size:        128     387     328

  Fragments arranged by size: 

                 387     328     128
 
StyI C'CwwG_G

Cuts at:      0     167     843 
   Size:        167     676
 
TatI w'GTAC_w

Cuts at:      0       2     843 
   Size:          2     841
 

 Enzymes that do cut:

     AccI    AlwNI    BanII     BbsI    BbvCI     BclI     BglI Bme1580I
   Bpu10I    BsaAI    BseYI  BsiHKAI     BsmI Bsp1286I    BspHI    BsrDI
    BsrGI   BstAPI    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
   DraIII     EaeI     EarI EcoO109I     MscI     MslI   MspA1I     NcoI
    PflMI     PstI    PvuII     SfcI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BcgI    BciVI    BfrBI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI     BpmI    BpuEI     BsaI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspMI
    BsrBI    BsrFI   BssHII    BssSI    BstBI   BstEII    BstYI     ClaI
    CspCI     DraI     DrdI     EagI     EciI  EcoICRI  Eco57MI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI