Restriction map for
pF1KE0807
(Linear) (Six-base) MAPSORT of: KIEE0807.seq Check: 2217 from: 1 to: 1083
KIEE0807.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 0 155 1083
Size: 155 928
AfeI AGC'GCT
Cuts at: 0 244 1083
Size: 244 839
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 391 430 433 451 1083
Size: 391 39 3 18 632
Fragments arranged by size:
632 391 39 18 3
AlwNI CAG_nnn'CTG
Cuts at: 0 546 1014 1083
Size: 546 468 69
Fragments arranged by size:
546 468 69
ApaI G_GGCC'C
Cuts at: 0 528 1083
Size: 528 555
ApaLI G'TGCA_C
Cuts at: 0 376 436 1083
Size: 376 60 647
Fragments arranged by size:
647 376 60
ApoI r'AATT_y
Cuts at: 0 621 1083
Size: 621 462
AvaI C'yCGr_G
Cuts at: 0 77 765 1083
Size: 77 688 318
Fragments arranged by size:
688 318 77
AvrII C'CTAG_G
Cuts at: 0 189 447 1083
Size: 189 258 636
Fragments arranged by size:
636 258 189
BanI G'GyrC_C
Cuts at: 0 508 1083
Size: 508 575
BanII G_rGCy'C
Cuts at: 0 57 528 1083
Size: 57 471 555
Fragments arranged by size:
555 471 57
BclI T'GATC_A
Cuts at: 0 723 1083
Size: 723 360
BglI GCCn_nnn'nGGC
Cuts at: 0 908 1083
Size: 908 175
BglII A'GATC_T
Cuts at: 0 1075 1083
Size: 1075 8
Bme1580I G_kGCm'C
Cuts at: 0 380 440 511 528 828 1083
Size: 380 60 71 17 300 255
Fragments arranged by size:
380 300 255 71 60 17
BmrI ACTGGGnnnn_n'
Cuts at: 0 953 1083
Size: 953 130
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 76 90 1018 1083
Size: 76 14 928 65
Fragments arranged by size:
928 76 65 14
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 267 523 991 1083
Size: 85 182 256 468 92
Fragments arranged by size:
468 256 182 92 85
BsaI GGTCTCn'nnnn_
Cuts at: 0 127 1083
Size: 127 956
BsaWI w'CCGG_w
Cuts at: 0 482 780 1083
Size: 482 298 303
Fragments arranged by size:
482 303 298
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 33 63 1083
Size: 33 30 1020
Fragments arranged by size:
1020 33 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 749 994 1083
Size: 749 245 89
Fragments arranged by size:
749 245 89
BseYI C'CCAG_C
Cuts at: 0 504 1045 1083
Size: 504 541 38
Fragments arranged by size:
541 504 38
BsiHKAI G_wGCw'C
Cuts at: 0 57 380 440 1083
Size: 57 323 60 643
Fragments arranged by size:
643 323 60 57
Bsp1286I G_dGCh'C
Cuts at: 0 57 380 440 511 528 828 1083
Size: 57 323 60 71 17 300 255
Fragments arranged by size:
323 300 255 71 60 57 17
BspEI T'CCGG_A
Cuts at: 0 780 1083
Size: 780 303
BspHI T'CATG_A
Cuts at: 0 744 1083
Size: 744 339
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 299 433 1083
Size: 299 134 650
Fragments arranged by size:
650 299 134
BstXI CCAn_nnnn'nTGG
Cuts at: 0 267 793 1083
Size: 267 526 290
Fragments arranged by size:
526 290 267
BstYI r'GATC_y
Cuts at: 0 1075 1083
Size: 1075 8
EaeI y'GGCC_r
Cuts at: 0 284 585 1083
Size: 284 301 498
Fragments arranged by size:
498 301 284
EarI CTCTTCn'nnn_
Cuts at: 0 812 1048 1083
Size: 812 236 35
Fragments arranged by size:
812 236 35
EcoICRI GAG'CTC
Cuts at: 0 55 1083
Size: 55 1028
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 267 523 991 1083
Size: 85 182 256 468 92
Fragments arranged by size:
468 256 182 92 85
EcoO109I rG'GnC_Cy
Cuts at: 0 525 1083
Size: 525 558
HaeII r_GCGC'y
Cuts at: 0 246 1083
Size: 246 837
MscI TGG'CCA
Cuts at: 0 587 1083
Size: 587 496
MslI CAynn'nnrTG
Cuts at: 0 433 692 1083
Size: 433 259 391
Fragments arranged by size:
433 391 259
MspA1I CmG'CkG
Cuts at: 0 543 948 1045 1083
Size: 543 405 97 38
Fragments arranged by size:
543 405 97 38
NspI r_CATG'y
Cuts at: 0 499 1083
Size: 499 584
PasI CC'CwG_GG
Cuts at: 0 1063 1083
Size: 1063 20
PflMI CCAn_nnn'nTGG
Cuts at: 0 205 245 1083
Size: 205 40 838
Fragments arranged by size:
838 205 40
PfoI T'CCnGG_A
Cuts at: 0 62 1083
Size: 62 1021
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 107 139 1083
Size: 107 32 944
Fragments arranged by size:
944 107 32
PspOMI G'GGCC_C
Cuts at: 0 524 1083
Size: 524 559
PvuII CAG'CTG
Cuts at: 0 543 948 1045 1083
Size: 543 405 97 38
Fragments arranged by size:
543 405 97 38
SacI G_AGCT'C
Cuts at: 0 57 1083
Size: 57 1026
SfcI C'TryA_G
Cuts at: 0 171 1083
Size: 171 912
SmaI CCC'GGG
Cuts at: 0 79 1083
Size: 79 1004
SphI G_CATG'C
Cuts at: 0 499 1083
Size: 499 584
StuI AGG'CCT
Cuts at: 0 928 1083
Size: 928 155
StyI C'CwwG_G
Cuts at: 0 189 447 681 1083
Size: 189 258 234 402
Fragments arranged by size:
402 258 234 189
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 476 1021 1083
Size: 476 545 62
Fragments arranged by size:
545 476 62
TatI w'GTAC_w
Cuts at: 0 143 966 1083
Size: 143 823 117
Fragments arranged by size:
823 143 117
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 33 65 1083
Size: 33 32 1018
Fragments arranged by size:
1018 33 32
Tth111I GACn'n_nGTC
Cuts at: 0 660 1083
Size: 660 423
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 264 525 1083
Size: 264 261 558
Fragments arranged by size:
558 264 261
XmaI C'CCGG_G
Cuts at: 0 77 1083
Size: 77 1006
Enzymes that do cut:
AccI AfeI AlfI AlwNI ApaI ApaLI ApoI AvaI
AvrII BanI BanII BclI BglI BglII Bme1580I BmrI
BplI BpmI BsaI BsaWI BsaXI BseRI BseYI BsiHKAI
Bsp1286I BspEI BspHI BspMI BstXI BstYI EaeI EarI
EcoICRI Eco57MI EcoO109I HaeII MscI MslI MspA1I NspI
PasI PflMI PfoI PpiI PspOMI PvuII SacI SfcI
SmaI SphI StuI StyI TaqII TatI TstI Tth111I
XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AloI AscI AseI AsiSI BaeI BamHI
BbeI BbsI BbvCI BcgI BciVI BfrBI BlpI BmgBI
BmtI Bpu10I BpuEI BsaAI BsaBI BsaHI BsgI BsiEI
BsiWI BsmI BsmBI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PmeI PmlI PpuMI PshAI PsiI
PspXI PsrI PstI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SrfI SspI SwaI XbaI XhoI
XmnI ZraI