(Linear) (Six-base) MAPSORT of: KIEE0781.seq  Check: 829  from: 1  to: 1050



KIEE0781.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:17  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     775     878    1050 
   Size:        775     103     172

  Fragments arranged by size: 

                 775     172     103
 
AflIII A'CryG_T

Cuts at:      0     584    1050 
   Size:        584     466
 
ApaLI G'TGCA_C

Cuts at:      0     967    1050 
   Size:        967      83
 
ApoI r'AATT_y

Cuts at:      0     100     322     684     718    1050 
   Size:        100     222     362      34     332

  Fragments arranged by size: 

                 362     332     222     100      34
 
BanI G'GyrC_C

Cuts at:      0     379    1050 
   Size:        379     671
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     815    1050 
   Size:        815     235
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     507    1050 
   Size:        507     543
 
BfrBI ATG'CAT

Cuts at:      0     305    1050 
   Size:        305     745
 
BglI GCCn_nnn'nGGC

Cuts at:      0     527    1050 
   Size:        527     523
 
Bme1580I G_kGCm'C

Cuts at:      0     971    1050 
   Size:        971      79
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     924    1050 
   Size:        924     126
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     277     599    1050 
   Size:        277     322     451

  Fragments arranged by size: 

                 451     322     277
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     822     894    1050 
   Size:        822      72     156

  Fragments arranged by size: 

                 822     156      72
 
BsaAI yAC'GTr

Cuts at:      0      59    1050 
   Size:         59     991
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     274    1050 
   Size:        274     776
 
BseYI C'CCAG_C

Cuts at:      0     841    1050 
   Size:        841     209
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     617     808    1050 
   Size:        617     191     242

  Fragments arranged by size: 

                 617     242     191
 
BsiHKAI G_wGCw'C

Cuts at:      0     160     971    1050 
   Size:        160     811      79

  Fragments arranged by size: 

                 811     160      79
 
Bsp1286I G_dGCh'C

Cuts at:      0     160     971    1050 
   Size:        160     811      79

  Fragments arranged by size: 

                 811     160      79
 
BspHI T'CATG_A

Cuts at:      0     533    1050 
   Size:        533     517
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     830    1050 
   Size:        830     220
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1029    1050 
   Size:       1029      21
 
BstYI r'GATC_y

Cuts at:      0     852    1050 
   Size:        852     198
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     530    1050 
   Size:        530     520
 
BtsI GCAGTG_nn'

Cuts at:      0     459     605    1050 
   Size:        459     146     445

  Fragments arranged by size: 

                 459     445     146
 
DraIII CAC_nnn'GTG

Cuts at:      0    1010    1050 
   Size:       1010      40
 
EarI CTCTTCn'nnn_

Cuts at:      0     674     916     926    1050 
   Size:        674     242      10     124

  Fragments arranged by size: 

                 674     242     124      10
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     277     599     775     878    1050 
   Size:        277     322     176     103     172

  Fragments arranged by size: 

                 322     277     176     172     103
 
EcoRI G'AATT_C

Cuts at:      0     100     684     718    1050 
   Size:        100     584      34     332

  Fragments arranged by size: 

                 584     332     100      34
 
EcoRV GAT'ATC

Cuts at:      0     285    1050 
   Size:        285     765
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     816     955    1050 
   Size:        816     139      95

  Fragments arranged by size: 

                 816     139      95
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     441     473    1050 
   Size:        441      32     577

  Fragments arranged by size: 

                 577     441      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     524    1050 
   Size:        524     526
 
MslI CAynn'nnrTG

Cuts at:      0     830    1027    1050 
   Size:        830     197      23

  Fragments arranged by size: 

                 830     197      23
 
MspA1I CmG'CkG

Cuts at:      0     147    1050 
   Size:        147     903
 
NsiI A_TGCA'T

Cuts at:      0     307    1050 
   Size:        307     743
 
NspI r_CATG'y

Cuts at:      0     588    1050 
   Size:        588     462
 
PasI CC'CwG_GG

Cuts at:      0     858    1050 
   Size:        858     192
 
PciI A'CATG_T

Cuts at:      0     584    1050 
   Size:        584     466
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     664    1050 
   Size:        664     386
 
PfoI T'CCnGG_A

Cuts at:      0     254     626    1050 
   Size:        254     372     424

  Fragments arranged by size: 

                 424     372     254
 
PstI C_TGCA'G

Cuts at:      0     572    1050 
   Size:        572     478
 
SfcI C'TryA_G

Cuts at:      0     568    1050 
   Size:        568     482
 
SmlI C'TyrA_G

Cuts at:      0     801     873    1050 
   Size:        801      72     177

  Fragments arranged by size: 

                 801     177      72
 
StuI AGG'CCT

Cuts at:      0     530    1050 
   Size:        530     520
 
StyI C'CwwG_G

Cuts at:      0     995    1050 
   Size:        995      55
 
XbaI T'CTAG_A

Cuts at:      0     432    1050 
   Size:        432     618
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     597    1050 
   Size:        597     453
 

 Enzymes that do cut:

     AcuI   AflIII    ApaLI     ApoI     BanI     BbsI    BciVI    BfrBI
     BglI Bme1580I     BplI     BpmI    BpuEI    BsaAI    BseRI    BseYI
     BsgI  BsiHKAI Bsp1286I    BspHI    BspMI    BstXI    BstYI    BtgZI
     BtsI   DraIII     EarI  Eco57MI    EcoRI    EcoRV     FalI    Hin4I
     MmeI     MslI   MspA1I     NsiI     NspI     PasI     PciI    PflMI
     PfoI     PstI     SfcI     SmlI     StuI     StyI     XbaI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI
     BcgI     BclI    BglII     BlpI    BmgBI     BmrI     BmtI   Bpu10I
     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI     ClaI    CspCI
     DraI     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     PacI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI    TaqII     TatI     TstI  Tth111I
     XhoI     XmaI     XmnI     ZraI