Restriction map for
pF1KE0768
(Linear) (Six-base) MAPSORT of: KIEE0768.seq Check: 7346 from: 1 to: 1314
KIEE0768.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 950 1262 1314
Size: 950 312 52
Fragments arranged by size:
950 312 52
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 656 712 1314
Size: 199 457 56 602
Fragments arranged by size:
602 457 199 56
AfeI AGC'GCT
Cuts at: 0 65 1314
Size: 65 1249
AflIII A'CryG_T
Cuts at: 0 1115 1314
Size: 1115 199
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 491 523 1314
Size: 491 32 791
Fragments arranged by size:
791 491 32
AlwNI CAG_nnn'CTG
Cuts at: 0 321 1314
Size: 321 993
ApaI G_GGCC'C
Cuts at: 0 719 1314
Size: 719 595
ApaLI G'TGCA_C
Cuts at: 0 1119 1314
Size: 1119 195
ApoI r'AATT_y
Cuts at: 0 981 1314
Size: 981 333
BanI G'GyrC_C
Cuts at: 0 926 1151 1250 1314
Size: 926 225 99 64
Fragments arranged by size:
926 225 99 64
BanII G_rGCy'C
Cuts at: 0 116 719 1069 1314
Size: 116 603 350 245
Fragments arranged by size:
603 350 245 116
BbsI GAAGACnn'nnnn_
Cuts at: 0 901 915 1314
Size: 901 14 399
Fragments arranged by size:
901 399 14
BbvCI CC'TCA_GC
Cuts at: 0 8 1314
Size: 8 1306
BciVI GTATCCnnnnn_n'
Cuts at: 0 579 1314
Size: 579 735
Bme1580I G_kGCm'C
Cuts at: 0 719 1095 1123 1207 1314
Size: 719 376 28 84 107
Fragments arranged by size:
719 376 107 84 28
BmrI ACTGGGnnnn_n'
Cuts at: 0 288 795 869 1193 1314
Size: 288 507 74 324 121
Fragments arranged by size:
507 324 288 121 74
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 724 1314
Size: 724 590
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 293 681 1314
Size: 193 100 388 633
Fragments arranged by size:
633 388 193 100
Bpu10I CC'TnA_GC
Cuts at: 0 8 1314
Size: 8 1306
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 868 1314
Size: 868 446
BsaI GGTCTCn'nnnn_
Cuts at: 0 861 1314
Size: 861 453
BsaAI yAC'GTr
Cuts at: 0 1116 1314
Size: 1116 198
BsaBI GATnn'nnATC
Cuts at: 0 81 1314
Size: 81 1233
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 166 193 196 223 395 425 1314
Size: 166 27 3 27 172 30 889
Fragments arranged by size:
889 172 166 30 27 27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 214 376 506 544 1314
Size: 214 162 130 38 770
Fragments arranged by size:
770 214 162 130 38
BseYI C'CCAG_C
Cuts at: 0 66 674 688 1289 1314
Size: 66 608 14 601 25
Fragments arranged by size:
608 601 66 25 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 278 1314
Size: 278 1036
BsiHKAI G_wGCw'C
Cuts at: 0 179 967 1123 1314
Size: 179 788 156 191
Fragments arranged by size:
788 191 179 156
BsmI GAATG_Cn'
Cuts at: 0 710 1314
Size: 710 604
Bsp1286I G_dGCh'C
Cuts at: 0 116 179 719 967 1069 1095 1123 1207
Size: 116 63 540 248 102 26 28 84
Cuts at: 1207 1314
Size: 107
Fragments arranged by size:
540 248 116 107 102 84 63 28
26
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 758 950 1262 1314
Size: 758 192 312 52
Fragments arranged by size:
758 312 192 52
BsrBI CCG'CTC
Cuts at: 0 144 1314
Size: 144 1170
BsrGI T'GTAC_A
Cuts at: 0 93 1314
Size: 93 1221
BstAPI GCAn_nnn'nTGC
Cuts at: 0 301 1040 1314
Size: 301 739 274
Fragments arranged by size:
739 301 274
BstEII G'GTnAC_C
Cuts at: 0 324 1314
Size: 324 990
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1057 1314
Size: 1057 257
BtgI C'CryG_G
Cuts at: 0 148 1314
Size: 148 1166
DraIII CAC_nnn'GTG
Cuts at: 0 1161 1314
Size: 1161 153
EaeI y'GGCC_r
Cuts at: 0 145 1004 1143 1227 1314
Size: 145 859 139 84 87
Fragments arranged by size:
859 145 139 87 84
EarI CTCTTCn'nnn_
Cuts at: 0 134 191 1129 1213 1314
Size: 134 57 938 84 101
Fragments arranged by size:
938 134 101 84 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 384 1314
Size: 384 930
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 199 293 656 681 712 1314
Size: 193 6 94 363 25 31 602
Fragments arranged by size:
602 363 193 94 31 25 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 196 1314
Size: 196 1118
EcoO109I rG'GnC_Cy
Cuts at: 0 166 318 507 715 716 1314
Size: 166 152 189 208 1 598
Fragments arranged by size:
598 208 189 166 152 1
HaeII r_GCGC'y
Cuts at: 0 67 1314
Size: 67 1247
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1 33 193 225 395 427 727 759
Size: 1 32 160 32 170 32 300 32
Cuts at: 759 902 934 1314
Size: 143 32 380
Fragments arranged by size:
380 300 170 160 143 32 32 32
32 32 1
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1038 1314
Size: 1038 276
MscI TGG'CCA
Cuts at: 0 1006 1314
Size: 1006 308
MslI CAynn'nnrTG
Cuts at: 0 766 944 1314
Size: 766 178 370
Fragments arranged by size:
766 370 178
MspA1I CmG'CkG
Cuts at: 0 99 292 878 1289 1314
Size: 99 193 586 411 25
Fragments arranged by size:
586 411 193 99 25
NcoI C'CATG_G
Cuts at: 0 148 1314
Size: 148 1166
NspI r_CATG'y
Cuts at: 0 474 797 1027 1314
Size: 474 323 230 287
Fragments arranged by size:
474 323 287 230
PasI CC'CwG_GG
Cuts at: 0 239 1314
Size: 239 1075
PmlI CAC'GTG
Cuts at: 0 1116 1314
Size: 1116 198
PpuMI rG'GwC_Cy
Cuts at: 0 166 507 1314
Size: 166 341 807
Fragments arranged by size:
807 341 166
PspOMI G'GGCC_C
Cuts at: 0 715 1314
Size: 715 599
PstI C_TGCA'G
Cuts at: 0 36 1314
Size: 36 1278
PvuII CAG'CTG
Cuts at: 0 99 292 878 1289 1314
Size: 99 193 586 411 25
Fragments arranged by size:
586 411 193 99 25
SanDI GG'GwC_CC
Cuts at: 0 507 1314
Size: 507 807
SapI GCTCTTCn'nnn_
Cuts at: 0 134 1129 1213 1314
Size: 134 995 84 101
Fragments arranged by size:
995 134 101 84
SfcI C'TryA_G
Cuts at: 0 32 873 1314
Size: 32 841 441
Fragments arranged by size:
841 441 32
SmlI C'TyrA_G
Cuts at: 0 883 1314
Size: 883 431
SphI G_CATG'C
Cuts at: 0 1027 1314
Size: 1027 287
StyI C'CwwG_G
Cuts at: 0 148 1314
Size: 148 1166
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1276 1314
Size: 1276 38
TatI w'GTAC_w
Cuts at: 0 93 1314
Size: 93 1221
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 166 198 1314
Size: 166 32 1116
Fragments arranged by size:
1116 166 32
Tth111I GACn'n_nGTC
Cuts at: 0 1274 1314
Size: 1274 40
XmnI GAAnn'nnTTC
Cuts at: 0 1195 1314
Size: 1195 119
Enzymes that do cut:
AarI AcuI AfeI AflIII AloI AlwNI ApaI ApaLI
ApoI BanI BanII BbsI BbvCI BciVI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI
BsrGI BstAPI BstEII BstXI BtgI DraIII EaeI EarI
EciI Eco57MI EcoNI EcoO109I HaeII Hin4I MmeI MscI
MslI MspA1I NcoI NspI PasI PmlI PpuMI PspOMI
PstI PvuII SanDI SapI SfcI SmlI SphI StyI
TaqII TatI TstI Tth111I XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AleI
AlfI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BbeI BcgI BclI BfrBI BglI BglII BlpI BmgBI
BmtI BsaHI BsaWI BsiEI BsiWI BsmBI BspEI BspHI
BsrDI BsrFI BssHII BssSI BstBI BstYI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DrdI EagI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
StuI SwaI XbaI XcmI XhoI XmaI ZraI