(Linear) (Six-base) MAPSORT of: KIEE0693.seq  Check: 8995  from: 1  to: 846



KIEE0693.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:17  ..

AatII G_ACGT'C

Cuts at:      0     699     846 
   Size:        699     147
 
ApoI r'AATT_y

Cuts at:      0     566     846 
   Size:        566     280
 
AvaI C'yCGr_G

Cuts at:      0     476     846 
   Size:        476     370
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     324     755     777     846 
   Size:        324     431      22      69

  Fragments arranged by size: 

                 431     324      69      22
 
BclI T'GATC_A

Cuts at:      0     456     846 
   Size:        456     390
 
BglII A'GATC_T

Cuts at:      0     817     846 
   Size:        817      29
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     476     846 
   Size:        476     370
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      60     206     260     846 
   Size:         60     146      54     586

  Fragments arranged by size: 

                 586     146      60      54
 
BsaBI GATnn'nnATC

Cuts at:      0     156     162     213     804     810     816     846 
   Size:        156       6      51     591       6       6      30

  Fragments arranged by size: 

                 591     156      51      30       6       6       6
 
BsaHI Gr'CG_yC

Cuts at:      0     696     846 
   Size:        696     150
 
BsaWI w'CCGG_w

Cuts at:      0     311     846 
   Size:        311     535
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      54      82      84     112     846 
   Size:         54      28       2      28     734

  Fragments arranged by size: 

                 734      54      28      28       2
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     674     846 
   Size:        674     172
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     231     846 
   Size:        231     615
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      86     846 
   Size:         86     760
 
BsrFI r'CCGG_y

Cuts at:      0     272     846 
   Size:        272     574
 
BsrGI T'GTAC_A

Cuts at:      0     383     846 
   Size:        383     463
 
BssSI C'ACGA_G

Cuts at:      0      77     846 
   Size:         77     769
 
BstYI r'GATC_y

Cuts at:      0     103     214     345     399     817     846 
   Size:        103     111     131      54     418      29

  Fragments arranged by size: 

                 418     131     111     103      54      29
 
EcoRI G'AATT_C

Cuts at:      0     566     846 
   Size:        566     280
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      82     114     846 
   Size:         82      32     732

  Fragments arranged by size: 

                 732      82      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      85     846 
   Size:         85     761
 
MslI CAynn'nnrTG

Cuts at:      0     622     644     846 
   Size:        622      22     202

  Fragments arranged by size: 

                 622     202      22
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     436     846 
   Size:        436     410
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      60      92     846 
   Size:         60      32     754

  Fragments arranged by size: 

                 754      60      32
 
SfcI C'TryA_G

Cuts at:      0     828     846 
   Size:        828      18
 
SmlI C'TyrA_G

Cuts at:      0     476     846 
   Size:        476     370
 
StyI C'CwwG_G

Cuts at:      0     360     846 
   Size:        360     486
 
TatI w'GTAC_w

Cuts at:      0     383     846 
   Size:        383     463
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     124     156     846 
   Size:        124      32     690

  Fragments arranged by size: 

                 690     124      32
 
XhoI C'TCGA_G

Cuts at:      0     476     846 
   Size:        476     370
 
XmnI GAAnn'nnTTC

Cuts at:      0     294     846 
   Size:        294     552
 
ZraI GAC'GTC

Cuts at:      0     697     846 
   Size:        697     149
 

 Enzymes that do cut:

    AatII     ApoI     AvaI     BbsI     BclI    BglII     BmrI     BplI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BsmBI    BspMI    BsrFI
    BsrGI    BssSI    BstYI    EcoRI    Hin4I     MmeI     MslI    PflMI
     PpiI     SfcI     SmlI     StyI     TatI     TstI     XhoI     XmnI
     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI     BlpI Bme1580I
    BmgBI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI Bsp1286I    BspEI    BspHI
    BsrBI    BsrDI   BssHII   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII  Tth111I
     XbaI     XcmI     XmaI