Restriction map for
pF1KE0684
(Linear) (Six-base) MAPSORT of: KIEE0684.seq Check: 9076 from: 1 to: 1197
KIEE0684.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AatII G_ACGT'C
Cuts at: 0 302 1197
Size: 302 895
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 450 835 1197
Size: 59 391 385 362
Fragments arranged by size:
391 385 362 59
AflII C'TTAA_G
Cuts at: 0 444 1197
Size: 444 753
AflIII A'CryG_T
Cuts at: 0 563 1157 1197
Size: 563 594 40
Fragments arranged by size:
594 563 40
ApoI r'AATT_y
Cuts at: 0 226 251 373 711 1197
Size: 226 25 122 338 486
Fragments arranged by size:
486 338 226 122 25
AvaI C'yCGr_G
Cuts at: 0 475 541 1086 1197
Size: 475 66 545 111
Fragments arranged by size:
545 475 111 66
BamHI G'GATC_C
Cuts at: 0 237 1197
Size: 237 960
BanII G_rGCy'C
Cuts at: 0 911 986 1093 1197
Size: 911 75 107 104
Fragments arranged by size:
911 107 104 75
BbsI GAAGACnn'nnnn_
Cuts at: 0 14 47 302 540 841 1197
Size: 14 33 255 238 301 356
Fragments arranged by size:
356 301 255 238 33 14
BbvCI CC'TCA_GC
Cuts at: 0 706 937 1197
Size: 706 231 260
Fragments arranged by size:
706 260 231
BmrI ACTGGGnnnn_n'
Cuts at: 0 300 1197
Size: 300 897
Bpu10I CC'TnA_GC
Cuts at: 0 706 937 1197
Size: 706 231 260
Fragments arranged by size:
706 260 231
BsaAI yAC'GTr
Cuts at: 0 566 573 1197
Size: 566 7 624
Fragments arranged by size:
624 566 7
BsaHI Gr'CG_yC
Cuts at: 0 299 1197
Size: 299 898
BsaWI w'CCGG_w
Cuts at: 0 1073 1197
Size: 1073 124
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 815 845 1197
Size: 815 30 352
Fragments arranged by size:
815 352 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 190 712 1111 1120 1197
Size: 190 522 399 9 77
Fragments arranged by size:
522 399 190 77 9
BseYI C'CCAG_C
Cuts at: 0 819 994 1197
Size: 819 175 203
Fragments arranged by size:
819 203 175
BsiEI CG_ry'CG
Cuts at: 0 798 1044 1197
Size: 798 246 153
Fragments arranged by size:
798 246 153
BsiHKAI G_wGCw'C
Cuts at: 0 986 1197
Size: 986 211
Bsp1286I G_dGCh'C
Cuts at: 0 911 986 1093 1197
Size: 911 75 107 104
Fragments arranged by size:
911 107 104 75
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 284 999 1197
Size: 284 715 198
Fragments arranged by size:
715 284 198
BsrFI r'CCGG_y
Cuts at: 0 848 1197
Size: 848 349
BsrGI T'GTAC_A
Cuts at: 0 435 1197
Size: 435 762
BstYI r'GATC_y
Cuts at: 0 237 746 1076 1143 1197
Size: 237 509 330 67 54
Fragments arranged by size:
509 330 237 67 54
Bsu36I CC'TnA_GG
Cuts at: 0 695 1197
Size: 695 502
BtgI C'CryG_G
Cuts at: 0 212 638 666 1197
Size: 212 426 28 531
Fragments arranged by size:
531 426 212 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 639 1197
Size: 639 558
DraIII CAC_nnn'GTG
Cuts at: 0 624 1197
Size: 624 573
EaeI y'GGCC_r
Cuts at: 0 1041 1197
Size: 1041 156
EagI C'GGCC_G
Cuts at: 0 1041 1197
Size: 1041 156
EarI CTCTTCn'nnn_
Cuts at: 0 50 1197
Size: 50 1147
EcoICRI GAG'CTC
Cuts at: 0 984 1197
Size: 984 213
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 450 835 1197
Size: 59 391 385 362
Fragments arranged by size:
391 385 362 59
EcoNI CCTnn'n_nnAGG
Cuts at: 0 694 1197
Size: 694 503
EcoO109I rG'GnC_Cy
Cuts at: 0 687 957 1197
Size: 687 270 240
Fragments arranged by size:
687 270 240
EcoRI G'AATT_C
Cuts at: 0 251 1197
Size: 251 946
HaeII r_GCGC'y
Cuts at: 0 849 1197
Size: 849 348
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 144 176 1197
Size: 144 32 1021
Fragments arranged by size:
1021 144 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 908 1197
Size: 908 289
MslI CAynn'nnrTG
Cuts at: 0 592 658 1197
Size: 592 66 539
Fragments arranged by size:
592 539 66
MspA1I CmG'CkG
Cuts at: 0 668 1197
Size: 668 529
NcoI C'CATG_G
Cuts at: 0 212 1197
Size: 212 985
NspI r_CATG'y
Cuts at: 0 1161 1197
Size: 1161 36
PasI CC'CwG_GG
Cuts at: 0 911 1197
Size: 911 286
PciI A'CATG_T
Cuts at: 0 1157 1197
Size: 1157 40
PfoI T'CCnGG_A
Cuts at: 0 749 1197
Size: 749 448
PmlI CAC'GTG
Cuts at: 0 566 1197
Size: 566 631
PpuMI rG'GwC_Cy
Cuts at: 0 687 957 1197
Size: 687 270 240
Fragments arranged by size:
687 270 240
PshAI GACnn'nnGTC
Cuts at: 0 80 1197
Size: 80 1117
PsiI TTA'TAA
Cuts at: 0 317 1197
Size: 317 880
SacI G_AGCT'C
Cuts at: 0 986 1197
Size: 986 211
SacII CC_GC'GG
Cuts at: 0 669 1197
Size: 669 528
SfcI C'TryA_G
Cuts at: 0 280 1197
Size: 280 917
SgrAI Cr'CCGG_yG
Cuts at: 0 848 1197
Size: 848 349
SmaI CCC'GGG
Cuts at: 0 477 543 1197
Size: 477 66 654
Fragments arranged by size:
654 477 66
SmlI C'TyrA_G
Cuts at: 0 444 1197
Size: 444 753
SspI AAT'ATT
Cuts at: 0 381 1197
Size: 381 816
StuI AGG'CCT
Cuts at: 0 329 1021 1197
Size: 329 692 176
Fragments arranged by size:
692 329 176
StyI C'CwwG_G
Cuts at: 0 212 1197
Size: 212 985
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 349 880 1197
Size: 349 531 317
Fragments arranged by size:
531 349 317
TatI w'GTAC_w
Cuts at: 0 435 1197
Size: 435 762
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 815 847 1197
Size: 815 32 350
Fragments arranged by size:
815 350 32
XbaI T'CTAG_A
Cuts at: 0 72 1197
Size: 72 1125
XmaI C'CCGG_G
Cuts at: 0 475 541 1197
Size: 475 66 656
Fragments arranged by size:
656 475 66
XmnI GAAnn'nnTTC
Cuts at: 0 68 1197
Size: 68 1129
ZraI GAC'GTC
Cuts at: 0 300 1197
Size: 300 897
Enzymes that do cut:
AatII AcuI AflII AflIII ApoI AvaI BamHI BanII
BbsI BbvCI BmrI Bpu10I BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspMI BsrFI BsrGI
BstYI Bsu36I BtgI BtgZI DraIII EaeI EagI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I MmeI
MslI MspA1I NcoI NspI PasI PciI PfoI PmlI
PpuMI PshAI PsiI SacI SacII SfcI SgrAI SmaI
SmlI SspI StuI StyI TaqII TatI TstI XbaI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BanI BbeI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmtI BplI BpmI
BpuEI BsaI BsaBI BsgI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrDI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I BtsI ClaI CspCI DraI DrdI EciI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MscI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PflMI PmeI
PpiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SnaBI SpeI SphI SrfI SwaI Tth111I XcmI
XhoI