Restriction map for
pF1KE0681
(Linear) (Six-base) MAPSORT of: KIEE0681.seq Check: 7792 from: 1 to: 810
KIEE0681.orf
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 276 349 810
Size: 241 35 73 461
Fragments arranged by size:
461 241 73 35
AleI CACnn'nnGTG
Cuts at: 0 190 451 810
Size: 190 261 359
Fragments arranged by size:
359 261 190
AlwNI CAG_nnn'CTG
Cuts at: 0 327 810
Size: 327 483
BbsI GAAGACnn'nnnn_
Cuts at: 0 683 810
Size: 683 127
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 646 680 810
Size: 646 34 130
Fragments arranged by size:
646 130 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 47 810
Size: 47 763
Bme1580I G_kGCm'C
Cuts at: 0 669 810
Size: 669 141
BmrI ACTGGGnnnn_n'
Cuts at: 0 3 810
Size: 3 807
Bpu10I CC'TnA_GC
Cuts at: 0 314 810
Size: 314 496
BsaI GGTCTCn'nnnn_
Cuts at: 0 504 690 810
Size: 504 186 120
Fragments arranged by size:
504 186 120
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 294 324 670 700 810
Size: 294 30 346 30 110
Fragments arranged by size:
346 294 110 30 30
BseYI C'CCAG_C
Cuts at: 0 783 810
Size: 783 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 739 775 810
Size: 739 36 35
Fragments arranged by size:
739 36 35
BsmI GAATG_Cn'
Cuts at: 0 621 782 810
Size: 621 161 28
Fragments arranged by size:
621 161 28
BsmBI CGTCTCn'nnnn_
Cuts at: 0 689 810
Size: 689 121
Bsp1286I G_dGCh'C
Cuts at: 0 669 810
Size: 669 141
BstXI CCAn_nnnn'nTGG
Cuts at: 0 574 810
Size: 574 236
BtgI C'CryG_G
Cuts at: 0 188 810
Size: 188 622
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 476 810
Size: 476 334
DraIII CAC_nnn'GTG
Cuts at: 0 495 810
Size: 495 315
DrdI GACnn_nn'nnGTC
Cuts at: 0 597 709 810
Size: 597 112 101
Fragments arranged by size:
597 112 101
EarI CTCTTCn'nnn_
Cuts at: 0 325 810
Size: 325 485
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 276 349 810
Size: 241 35 73 461
Fragments arranged by size:
461 241 73 35
EcoO109I rG'GnC_Cy
Cuts at: 0 638 810
Size: 638 172
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 198 810
Size: 198 612
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 503 535 670 702 810
Size: 503 32 135 32 108
Fragments arranged by size:
503 135 108 32 32
HincII GTy'rAC
Cuts at: 0 54 714 810
Size: 54 660 96
Fragments arranged by size:
660 96 54
MslI CAynn'nnrTG
Cuts at: 0 190 451 810
Size: 190 261 359
Fragments arranged by size:
359 261 190
NspI r_CATG'y
Cuts at: 0 585 810
Size: 585 225
PfoI T'CCnGG_A
Cuts at: 0 540 810
Size: 540 270
PpuMI rG'GwC_Cy
Cuts at: 0 638 810
Size: 638 172
SfcI C'TryA_G
Cuts at: 0 319 810
Size: 319 491
StyI C'CwwG_G
Cuts at: 0 567 810
Size: 567 243
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 463 810
Size: 463 347
TatI w'GTAC_w
Cuts at: 0 254 810
Size: 254 556
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 17 49 294 326 810
Size: 17 32 245 32 484
Fragments arranged by size:
484 245 32 32 17
Tth111I GACn'n_nGTC
Cuts at: 0 682 810
Size: 682 128
XmnI GAAnn'nnTTC
Cuts at: 0 212 810
Size: 212 598
Enzymes that do cut:
AcuI AleI AlwNI BbsI BcgI BciVI Bme1580I BmrI
Bpu10I BsaI BsaXI BseYI BsgI BsmI BsmBI Bsp1286I
BstXI BtgI BtgZI DraIII DrdI EarI Eco57MI EcoO109I
FalI Hin4I HincII MslI NspI PfoI PpuMI SfcI
StyI TaqII TatI TstI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbvCI BclI BfrBI BglI BglII BlpI BmgBI
BmtI BplI BpmI BpuEI BsaAI BsaBI BsaHI BsaWI
BseRI BsiEI BsiHKAI BsiWI BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BstZ17I Bsu36I BtsI ClaI CspCI DraI EaeI
EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI
FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI XbaI XcmI XhoI XmaI ZraI