Restriction map for
pF1KE0632
(Linear) (Six-base) MAPSORT of: KIEE0632.seq Check: 3132 from: 1 to: 1128
KIEE0632.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AatII G_ACGT'C
Cuts at: 0 902 1128
Size: 902 226
Acc65I G'GTAC_C
Cuts at: 0 594 1128
Size: 594 534
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 640 1128
Size: 640 488
AlwNI CAG_nnn'CTG
Cuts at: 0 1002 1041 1128
Size: 1002 39 87
Fragments arranged by size:
1002 87 39
ApaI G_GGCC'C
Cuts at: 0 1115 1128
Size: 1115 13
ApaLI G'TGCA_C
Cuts at: 0 148 199 386 1128
Size: 148 51 187 742
Fragments arranged by size:
742 187 148 51
ApoI r'AATT_y
Cuts at: 0 82 1128
Size: 82 1046
AscI GG'CGCG_CC
Cuts at: 0 950 1128
Size: 950 178
AvaI C'yCGr_G
Cuts at: 0 457 598 795 945 971 1128
Size: 457 141 197 150 26 157
Fragments arranged by size:
457 197 157 150 141 26
BanI G'GyrC_C
Cuts at: 0 529 567 594 814 1128
Size: 529 38 27 220 314
Fragments arranged by size:
529 314 220 38 27
BanII G_rGCy'C
Cuts at: 0 328 1115 1128
Size: 328 787 13
Fragments arranged by size:
787 328 13
BbeI G_GCGC'C
Cuts at: 0 533 1128
Size: 533 595
BbsI GAAGACnn'nnnn_
Cuts at: 0 270 685 1128
Size: 270 415 443
Fragments arranged by size:
443 415 270
BlpI GC'TnA_GC
Cuts at: 0 1045 1128
Size: 1045 83
Bme1580I G_kGCm'C
Cuts at: 0 152 203 390 572 916 1115 1128
Size: 152 51 187 182 344 199 13
Fragments arranged by size:
344 199 187 182 152 51 13
BmgBI CAC'GTC
Cuts at: 0 931 1128
Size: 931 197
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 670 940 1018 1128
Size: 580 90 270 78 110
Fragments arranged by size:
580 270 110 90 78
Bpu10I CC'TnA_GC
Cuts at: 0 271 1128
Size: 271 857
BsaI GGTCTCn'nnnn_
Cuts at: 0 200 1128
Size: 200 928
BsaHI Gr'CG_yC
Cuts at: 0 530 899 1128
Size: 530 369 229
Fragments arranged by size:
530 369 229
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 148 178 1128
Size: 148 30 950
Fragments arranged by size:
950 148 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 310 1117 1128
Size: 310 807 11
Fragments arranged by size:
807 310 11
BseYI C'CCAG_C
Cuts at: 0 351 507 1128
Size: 351 156 621
Fragments arranged by size:
621 351 156
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 354 466 911 1128
Size: 354 112 445 217
Fragments arranged by size:
445 354 217 112
BsiEI CG_ry'CG
Cuts at: 0 780 1128
Size: 780 348
BsiHKAI G_wGCw'C
Cuts at: 0 152 203 390 619 1109 1128
Size: 152 51 187 229 490 19
Fragments arranged by size:
490 229 187 152 51 19
BsmBI CGTCTCn'nnnn_
Cuts at: 0 454 1128
Size: 454 674
Bsp1286I G_dGCh'C
Cuts at: 0 152 203 328 390 572 619 916 1109
Size: 152 51 125 62 182 47 297 193
Cuts at: 1109 1115 1128
Size: 6 13
Fragments arranged by size:
297 193 182 152 125 62 51 47
13 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 218 1128
Size: 218 910
BsrFI r'CCGG_y
Cuts at: 0 467 485 1128
Size: 467 18 643
Fragments arranged by size:
643 467 18
BssHII G'CGCG_C
Cuts at: 0 356 950 1128
Size: 356 594 178
Fragments arranged by size:
594 356 178
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1002 1128
Size: 1002 126
BtgI C'CryG_G
Cuts at: 0 818 1115 1128
Size: 818 297 13
Fragments arranged by size:
818 297 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 539 899 1128
Size: 539 360 229
Fragments arranged by size:
539 360 229
BtsI GCAGTG_nn'
Cuts at: 0 59 1128
Size: 59 1069
ClaI AT'CG_AT
Cuts at: 0 440 1128
Size: 440 688
DrdI GACnn_nn'nnGTC
Cuts at: 0 898 1128
Size: 898 230
EaeI y'GGCC_r
Cuts at: 0 671 777 1128
Size: 671 106 351
Fragments arranged by size:
671 351 106
EagI C'GGCC_G
Cuts at: 0 777 1128
Size: 777 351
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 94 354 409 625 892 1128
Size: 94 260 55 216 267 236
Fragments arranged by size:
267 260 236 216 94 55
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 670 940 1018 1128
Size: 580 90 270 78 110
Fragments arranged by size:
580 270 110 90 78
EcoO109I rG'GnC_Cy
Cuts at: 0 373 603 645 832 1011 1128
Size: 373 230 42 187 179 117
Fragments arranged by size:
373 230 187 179 117 42
EcoRI G'AATT_C
Cuts at: 0 82 1128
Size: 82 1046
HaeII r_GCGC'y
Cuts at: 0 352 533 1084 1128
Size: 352 181 551 44
Fragments arranged by size:
551 352 181 44
KasI G'GCGC_C
Cuts at: 0 529 1128
Size: 529 599
KpnI G_GTAC'C
Cuts at: 0 598 1128
Size: 598 530
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 1128
Size: 191 937
MslI CAynn'nnrTG
Cuts at: 0 442 667 700 1128
Size: 442 225 33 428
Fragments arranged by size:
442 428 225 33
MspA1I CmG'CkG
Cuts at: 0 507 820 999 1008 1038 1117 1128
Size: 507 313 179 9 30 79 11
Fragments arranged by size:
507 313 179 79 30 11 9
NarI GG'CG_CC
Cuts at: 0 530 1128
Size: 530 598
NotI GC'GGCC_GC
Cuts at: 0 777 1128
Size: 777 351
PfoI T'CCnGG_A
Cuts at: 0 647 1128
Size: 647 481
PpuMI rG'GwC_Cy
Cuts at: 0 373 645 832 1011 1128
Size: 373 272 187 179 117
Fragments arranged by size:
373 272 187 179 117
PshAI GACnn'nnGTC
Cuts at: 0 1067 1128
Size: 1067 61
PspOMI G'GGCC_C
Cuts at: 0 1111 1128
Size: 1111 17
PstI C_TGCA'G
Cuts at: 0 857 1007 1128
Size: 857 150 121
Fragments arranged by size:
857 150 121
SacII CC_GC'GG
Cuts at: 0 821 1118 1128
Size: 821 297 10
Fragments arranged by size:
821 297 10
SanDI GG'GwC_CC
Cuts at: 0 832 1128
Size: 832 296
SexAI A'CCwGG_T
Cuts at: 0 449 689 1128
Size: 449 240 439
Fragments arranged by size:
449 439 240
SfcI C'TryA_G
Cuts at: 0 853 1003 1128
Size: 853 150 125
Fragments arranged by size:
853 150 125
SfoI GGC'GCC
Cuts at: 0 531 1128
Size: 531 597
SgrAI Cr'CCGG_yG
Cuts at: 0 467 1128
Size: 467 661
SmaI CCC'GGG
Cuts at: 0 600 947 973 1128
Size: 600 347 26 155
Fragments arranged by size:
600 347 155 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 169 1128
Size: 169 959
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 148 180 1128
Size: 148 32 948
Fragments arranged by size:
948 148 32
Tth111I GACn'n_nGTC
Cuts at: 0 451 643 691 901 1128
Size: 451 192 48 210 227
Fragments arranged by size:
451 227 210 192 48
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 441 1128
Size: 441 687
XmaI C'CCGG_G
Cuts at: 0 598 945 971 1128
Size: 598 347 26 157
Fragments arranged by size:
598 347 157 26
ZraI GAC'GTC
Cuts at: 0 900 1128
Size: 900 228
Enzymes that do cut:
AatII Acc65I AlfI AlwNI ApaI ApaLI ApoI AscI
AvaI BanI BanII BbeI BbsI BlpI Bme1580I BmgBI
BpmI Bpu10I BsaI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BssHII BstAPI
BtgI BtgZI BtsI ClaI DrdI EaeI EagI EciI
Eco57MI EcoO109I EcoRI HaeII KasI KpnI MmeI MslI
MspA1I NarI NotI PfoI PpuMI PshAI PspOMI PstI
SacII SanDI SexAI SfcI SfoI SgrAI SmaI TaqII
TstI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
AarI AccI AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AloI AseI AsiSI AvrII BaeI BamHI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BmrI
BmtI BplI BpuEI BsaAI BsaBI BsaWI BsiWI BsmI
BspEI BspHI BsrBI BsrDI BsrGI BssSI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I CspCI DraI DraIII EarI
EcoICRI EcoNI EcoRV FalI FseI FspI FspAI Hin4I
HincII HindIII HpaI MfeI MluI MscI NaeI NcoI
NdeI NgoMIV NheI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PmlI PpiI PsiI PspXI PsrI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfiI SgfI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TatI XbaI XhoI XmnI