(Linear) (Six-base) MAPSORT of: KIEE0568.seq  Check: 5461  from: 1  to: 864



KIEE0568.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     608     864 
   Size:        608     256
 
AlwNI CAG_nnn'CTG

Cuts at:      0     146     602     659     716     746     803     864 
   Size:        146     456      57      57      30      57      61

  Fragments arranged by size: 

                 456     146      61      57      57      57      30
 
BanII G_rGCy'C

Cuts at:      0     229     319     379     499     559     864 
   Size:        229      90      60     120      60     305

  Fragments arranged by size: 

                 305     229     120      90      60      60
 
Bme1580I G_kGCm'C

Cuts at:      0     155     864 
   Size:        155     709
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     585     816     864 
   Size:        585     231      48

  Fragments arranged by size: 

                 585     231      48
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      41     123     189     864 
   Size:         41      82      66     675

  Fragments arranged by size: 

                 675      82      66      41
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     666     669     753     756     864 
   Size:        666       3      84       3     108

  Fragments arranged by size: 

                 666     108      84       3       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     615     864 
   Size:        615     249
 
Bsp1286I G_dGCh'C

Cuts at:      0     155     229     319     379     499     559     864 
   Size:        155      74      90      60     120      60     305

  Fragments arranged by size: 

                 305     155     120      90      74      60      60
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     812     864 
   Size:        812      52
 
Bsu36I CC'TnA_GG

Cuts at:      0     681     768     864 
   Size:        681      87      96

  Fragments arranged by size: 

                 681      96      87
 
EarI CTCTTCn'nnn_

Cuts at:      0     627     864 
   Size:        627     237
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      41     123     189     608     864 
   Size:         41      82      66     419     256

  Fragments arranged by size: 

                 419     256      82      66      41
 
MspA1I CmG'CkG

Cuts at:      0     143     209     599     656     713     743     864 
   Size:        143      66     390      57      57      30     121

  Fragments arranged by size: 

                 390     143     121      66      57      57      30
 
PvuII CAG'CTG

Cuts at:      0     143     209     599     656     713     743     864 
   Size:        143      66     390      57      57      30     121

  Fragments arranged by size: 

                 390     143     121      66      57      57      30
 
SapI GCTCTTCn'nnn_

Cuts at:      0     627     864 
   Size:        627     237
 
StyI C'CwwG_G

Cuts at:      0     230     260     290     320     350     380     410     440 
   Size:        230      30      30      30      30      30      30      30

Cuts at:    440     470     500     530     864 
   Size:         30      30      30     334

  Fragments arranged by size: 

                 334     230      30      30      30      30      30      30
                  30      30      30      30
 

 Enzymes that do cut:

     AcuI    AlwNI    BanII Bme1580I     BmrI     BpmI    BseRI     BsgI
 Bsp1286I   BstAPI   Bsu36I     EarI  Eco57MI   MspA1I    PvuII     SapI
     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI
  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI