Restriction map for
pF1KE0562
(Linear) (Six-base) MAPSORT of: KIEE0562.seq Check: 1810 from: 1 to: 984
KIEE0562.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 0 101 984
Size: 101 883
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 984
Size: 280 704
AlwNI CAG_nnn'CTG
Cuts at: 0 36 687 984
Size: 36 651 297
Fragments arranged by size:
651 297 36
BanI G'GyrC_C
Cuts at: 0 331 984
Size: 331 653
BanII G_rGCy'C
Cuts at: 0 636 800 984
Size: 636 164 184
Fragments arranged by size:
636 184 164
BbeI G_GCGC'C
Cuts at: 0 335 984
Size: 335 649
BbsI GAAGACnn'nnnn_
Cuts at: 0 175 268 379 753 984
Size: 175 93 111 374 231
Fragments arranged by size:
374 231 175 111 93
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 588 622 984
Size: 588 34 362
Fragments arranged by size:
588 362 34
BclI T'GATC_A
Cuts at: 0 678 984
Size: 678 306
BlpI GC'TnA_GC
Cuts at: 0 499 984
Size: 499 485
Bme1580I G_kGCm'C
Cuts at: 0 569 984
Size: 569 415
BmrI ACTGGGnnnn_n'
Cuts at: 0 803 984
Size: 803 181
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 607 795 984
Size: 574 33 188 189
Fragments arranged by size:
574 189 188 33
Bpu10I CC'TnA_GC
Cuts at: 0 281 984
Size: 281 703
BsaI GGTCTCn'nnnn_
Cuts at: 0 955 984
Size: 955 29
BsaHI Gr'CG_yC
Cuts at: 0 332 984
Size: 332 652
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 383 984
Size: 383 601
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 333 901 984
Size: 333 568 83
Fragments arranged by size:
568 333 83
BsiHKAI G_wGCw'C
Cuts at: 0 287 469 984
Size: 287 182 515
Fragments arranged by size:
515 287 182
Bsp1286I G_dGCh'C
Cuts at: 0 287 469 569 636 800 984
Size: 287 182 100 67 164 184
Fragments arranged by size:
287 184 182 164 100 67
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 113 984
Size: 113 871
BsrGI T'GTAC_A
Cuts at: 0 710 984
Size: 710 274
BssSI C'ACGA_G
Cuts at: 0 520 984
Size: 520 464
BstAPI GCAn_nnn'nTGC
Cuts at: 0 347 564 984
Size: 347 217 420
Fragments arranged by size:
420 347 217
BstXI CCAn_nnnn'nTGG
Cuts at: 0 511 984
Size: 511 473
BstYI r'GATC_y
Cuts at: 0 80 425 984
Size: 80 345 559
Fragments arranged by size:
559 345 80
BtgI C'CryG_G
Cuts at: 0 312 504 984
Size: 312 192 480
Fragments arranged by size:
480 312 192
BtsI GCAGTG_nn'
Cuts at: 0 55 847 984
Size: 55 792 137
Fragments arranged by size:
792 137 55
EaeI y'GGCC_r
Cuts at: 0 124 401 452 984
Size: 124 277 51 532
Fragments arranged by size:
532 277 124 51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 721 984
Size: 721 263
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 574 607 795 984
Size: 280 294 33 188 189
Fragments arranged by size:
294 280 189 188 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 204 435 984
Size: 204 231 549
Fragments arranged by size:
549 231 204
EcoO109I rG'GnC_Cy
Cuts at: 0 270 552 984
Size: 270 282 432
Fragments arranged by size:
432 282 270
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 447 984
Size: 447 537
FspI TGC'GCA
Cuts at: 0 613 984
Size: 613 371
FspAI rTGC'GCAy
Cuts at: 0 613 984
Size: 613 371
HaeII r_GCGC'y
Cuts at: 0 335 984
Size: 335 649
HindIII A'AGCT_T
Cuts at: 0 774 984
Size: 774 210
KasI G'GCGC_C
Cuts at: 0 331 984
Size: 331 653
MscI TGG'CCA
Cuts at: 0 403 454 984
Size: 403 51 530
Fragments arranged by size:
530 403 51
MslI CAynn'nnrTG
Cuts at: 0 120 311 532 984
Size: 120 191 221 452
Fragments arranged by size:
452 221 191 120
NarI GG'CG_CC
Cuts at: 0 332 984
Size: 332 652
NcoI C'CATG_G
Cuts at: 0 312 504 984
Size: 312 192 480
Fragments arranged by size:
480 312 192
PflMI CCAn_nnn'nTGG
Cuts at: 0 77 879 984
Size: 77 802 105
Fragments arranged by size:
802 105 77
PfoI T'CCnGG_A
Cuts at: 0 158 984
Size: 158 826
PpuMI rG'GwC_Cy
Cuts at: 0 270 984
Size: 270 714
PstI C_TGCA'G
Cuts at: 0 50 984
Size: 50 934
SfcI C'TryA_G
Cuts at: 0 46 984
Size: 46 938
SfoI GGC'GCC
Cuts at: 0 333 984
Size: 333 651
StuI AGG'CCT
Cuts at: 0 816 984
Size: 816 168
StyI C'CwwG_G
Cuts at: 0 312 504 984
Size: 312 192 480
Fragments arranged by size:
480 312 192
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 535 984
Size: 535 449
TatI w'GTAC_w
Cuts at: 0 710 984
Size: 710 274
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 201 233 984
Size: 201 32 751
Fragments arranged by size:
751 201 32
XmnI GAAnn'nnTTC
Cuts at: 0 376 984
Size: 376 608
Enzymes that do cut:
AccI AcuI AlwNI BanI BanII BbeI BbsI BcgI
BclI BlpI Bme1580I BmrI BpmI Bpu10I BsaI BsaHI
BseRI BsgI BsiHKAI Bsp1286I BspMI BsrGI BssSI BstAPI
BstXI BstYI BtgI BtsI EaeI EciI Eco57MI EcoNI
EcoO109I FalI FspI FspAI HaeII HindIII KasI MscI
MslI NarI NcoI PflMI PfoI PpuMI PstI SfcI
SfoI StuI StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbvCI BciVI
BfrBI BglI BglII BmgBI BmtI BplI BpuEI BsaAI
BsaBI BsaWI BsaXI BseYI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrDI BsrFI BssHII BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EagI EarI EcoICRI EcoRI EcoRV FseI Hin4I HincII
HpaI KpnI MfeI MluI MmeI MspA1I NaeI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI SwaI Tth111I
XbaI XcmI XhoI XmaI ZraI