Restriction map for
pF1KE0556
(Linear) (Six-base) MAPSORT of: KIEE0556.seq Check: 317 from: 1 to: 753
KIEE0556.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 753
Size: 145 608
AhdI GACnn_n'nnGTC
Cuts at: 0 154 753
Size: 154 599
AlwNI CAG_nnn'CTG
Cuts at: 0 39 53 169 753
Size: 39 14 116 584
Fragments arranged by size:
584 116 39 14
ApaI G_GGCC'C
Cuts at: 0 192 589 654 753
Size: 192 397 65 99
Fragments arranged by size:
397 192 99 65
ApoI r'AATT_y
Cuts at: 0 601 753
Size: 601 152
BanII G_rGCy'C
Cuts at: 0 113 192 589 654 753
Size: 113 79 397 65 99
Fragments arranged by size:
397 113 99 79 65
BbvCI CC'TCA_GC
Cuts at: 0 216 497 568 753
Size: 216 281 71 185
Fragments arranged by size:
281 216 185 71
BglI GCCn_nnn'nGGC
Cuts at: 0 117 220 753
Size: 117 103 533
Fragments arranged by size:
533 117 103
Bme1580I G_kGCm'C
Cuts at: 0 33 192 589 654 753
Size: 33 159 397 65 99
Fragments arranged by size:
397 159 99 65 33
Bpu10I CC'TnA_GC
Cuts at: 0 216 497 568 753
Size: 216 281 71 185
Fragments arranged by size:
281 216 185 71
BsaI GGTCTCn'nnnn_
Cuts at: 0 367 753
Size: 367 386
BsaAI yAC'GTr
Cuts at: 0 666 753
Size: 666 87
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 271 475 585 753
Size: 271 204 110 168
Fragments arranged by size:
271 204 168 110
BseYI C'CCAG_C
Cuts at: 0 57 291 504 582 747 753
Size: 57 234 213 78 165 6
Fragments arranged by size:
234 213 165 78 57 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 66 568 688 753
Size: 66 502 120 65
Fragments arranged by size:
502 120 66 65
Bsp1286I G_dGCh'C
Cuts at: 0 33 113 192 589 654 753
Size: 33 80 79 397 65 99
Fragments arranged by size:
397 99 80 79 65 33
BsrDI GCAATG_nn'
Cuts at: 0 203 753
Size: 203 550
BsrFI r'CCGG_y
Cuts at: 0 43 753
Size: 43 710
BsrGI T'GTAC_A
Cuts at: 0 314 753
Size: 314 439
BstAPI GCAn_nnn'nTGC
Cuts at: 0 708 753
Size: 708 45
BstEII G'GTnAC_C
Cuts at: 0 201 753
Size: 201 552
BstYI r'GATC_y
Cuts at: 0 716 753
Size: 716 37
BtgI C'CryG_G
Cuts at: 0 227 276 323 753
Size: 227 49 47 430
Fragments arranged by size:
430 227 49 47
EaeI y'GGCC_r
Cuts at: 0 369 753
Size: 369 384
EarI CTCTTCn'nnn_
Cuts at: 0 277 753
Size: 277 476
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 753
Size: 145 608
EcoNI CCTnn'n_nnAGG
Cuts at: 0 391 753
Size: 391 362
EcoO109I rG'GnC_Cy
Cuts at: 0 62 189 281 300 586 651 753
Size: 62 127 92 19 286 65 102
Fragments arranged by size:
286 127 102 92 65 62 19
FspI TGC'GCA
Cuts at: 0 103 753
Size: 103 650
HaeII r_GCGC'y
Cuts at: 0 578 745 753
Size: 578 167 8
Fragments arranged by size:
578 167 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 140 172 753
Size: 140 32 581
Fragments arranged by size:
581 140 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 425 753
Size: 425 328
MslI CAynn'nnrTG
Cuts at: 0 163 753
Size: 163 590
MspA1I CmG'CkG
Cuts at: 0 220 291 477 496 552 567 672 739
Size: 220 71 186 19 56 15 105 67
Cuts at: 739 753
Size: 14
Fragments arranged by size:
220 186 105 71 67 56 19 15
14
NcoI C'CATG_G
Cuts at: 0 227 276 753
Size: 227 49 477
Fragments arranged by size:
477 227 49
PasI CC'CwG_GG
Cuts at: 0 25 753
Size: 25 728
PfoI T'CCnGG_A
Cuts at: 0 719 753
Size: 719 34
PmlI CAC'GTG
Cuts at: 0 666 753
Size: 666 87
PpuMI rG'GwC_Cy
Cuts at: 0 62 281 753
Size: 62 219 472
Fragments arranged by size:
472 219 62
PspOMI G'GGCC_C
Cuts at: 0 188 585 650 753
Size: 188 397 65 103
Fragments arranged by size:
397 188 103 65
PstI C_TGCA'G
Cuts at: 0 290 476 482 753
Size: 290 186 6 271
Fragments arranged by size:
290 271 186 6
PvuII CAG'CTG
Cuts at: 0 291 477 496 567 753
Size: 291 186 19 71 186
Fragments arranged by size:
291 186 186 71 19
SanDI GG'GwC_CC
Cuts at: 0 281 753
Size: 281 472
SapI GCTCTTCn'nnn_
Cuts at: 0 277 753
Size: 277 476
SfcI C'TryA_G
Cuts at: 0 286 472 478 753
Size: 286 186 6 275
Fragments arranged by size:
286 275 186 6
StyI C'CwwG_G
Cuts at: 0 227 276 753
Size: 227 49 477
Fragments arranged by size:
477 227 49
TatI w'GTAC_w
Cuts at: 0 77 314 753
Size: 77 237 439
Fragments arranged by size:
439 237 77
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 354 753
Size: 354 399
Enzymes that do cut:
AcuI AhdI AlwNI ApaI ApoI BanII BbvCI BglI
Bme1580I Bpu10I BsaI BsaAI BseRI BseYI BsgI Bsp1286I
BsrDI BsrFI BsrGI BstAPI BstEII BstYI BtgI EaeI
EarI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I MmeI
MslI MspA1I NcoI PasI PfoI PmlI PpuMI PspOMI
PstI PvuII SanDI SapI SfcI StyI TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbsI BcgI
BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI
BplI BpmI BpuEI BsaBI BsaHI BsaWI BsaXI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BssHII BssSI BstBI BstXI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI
EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MscI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PciI
PflMI PmeI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TstI Tth111I XbaI
XhoI XmaI XmnI ZraI