Restriction map for
pF1KE0546
(Linear) (Six-base) MAPSORT of: KIEE0546.seq Check: 7918 from: 1 to: 891
KIEE0546.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 489 891
Size: 489 402
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 891
Size: 424 467
AflIII A'CryG_T
Cuts at: 0 98 891
Size: 98 793
AhdI GACnn_n'nnGTC
Cuts at: 0 201 891
Size: 201 690
AleI CACnn'nnGTG
Cuts at: 0 283 891
Size: 283 608
AlwNI CAG_nnn'CTG
Cuts at: 0 504 891
Size: 504 387
ApaI G_GGCC'C
Cuts at: 0 80 676 849 891
Size: 80 596 173 42
Fragments arranged by size:
596 173 80 42
AvaI C'yCGr_G
Cuts at: 0 122 145 329 603 891
Size: 122 23 184 274 288
Fragments arranged by size:
288 274 184 122 23
BanI G'GyrC_C
Cuts at: 0 118 163 568 619 891
Size: 118 45 405 51 272
Fragments arranged by size:
405 272 118 51 45
BanII G_rGCy'C
Cuts at: 0 80 434 676 686 849 891
Size: 80 354 242 10 163 42
Fragments arranged by size:
354 242 163 80 42 10
BbeI G_GCGC'C
Cuts at: 0 167 891
Size: 167 724
BbsI GAAGACnn'nnnn_
Cuts at: 0 447 891
Size: 447 444
BglI GCCn_nnn'nGGC
Cuts at: 0 263 891
Size: 263 628
Bme1580I G_kGCm'C
Cuts at: 0 80 571 676 849 891
Size: 80 491 105 173 42
Fragments arranged by size:
491 173 105 80 42
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 891
Size: 526 365
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 536 891
Size: 469 67 355
Fragments arranged by size:
469 355 67
BsaI GGTCTCn'nnnn_
Cuts at: 0 36 554 891
Size: 36 518 337
Fragments arranged by size:
518 337 36
BsaAI yAC'GTr
Cuts at: 0 156 285 740 768 891
Size: 156 129 455 28 123
Fragments arranged by size:
455 156 129 123 28
BsaHI Gr'CG_yC
Cuts at: 0 164 202 891
Size: 164 38 689
Fragments arranged by size:
689 164 38
BsaWI w'CCGG_w
Cuts at: 0 392 891
Size: 392 499
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 805 835 891
Size: 805 30 56
Fragments arranged by size:
805 56 30
BseYI C'CCAG_C
Cuts at: 0 72 226 891
Size: 72 154 665
Fragments arranged by size:
665 154 72
BsiEI CG_ry'CG
Cuts at: 0 452 891
Size: 452 439
BsiHKAI G_wGCw'C
Cuts at: 0 17 380 686 693 891
Size: 17 363 306 7 198
Fragments arranged by size:
363 306 198 17 7
Bsp1286I G_dGCh'C
Cuts at: 0 17 80 380 434 571 676 686 693
Size: 17 63 300 54 137 105 10 7
Cuts at: 693 849 891
Size: 156 42
Fragments arranged by size:
300 156 137 105 63 54 42 17
10 7
BspEI T'CCGG_A
Cuts at: 0 392 891
Size: 392 499
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 489 891
Size: 489 402
BsrBI CCG'CTC
Cuts at: 0 476 891
Size: 476 415
BsrDI GCAATG_nn'
Cuts at: 0 731 891
Size: 731 160
BsrFI r'CCGG_y
Cuts at: 0 447 891
Size: 447 444
BstXI CCAn_nnnn'nTGG
Cuts at: 0 45 616 711 891
Size: 45 571 95 180
Fragments arranged by size:
571 180 95 45
BstYI r'GATC_y
Cuts at: 0 714 891
Size: 714 177
BtgI C'CryG_G
Cuts at: 0 615 891
Size: 615 276
DraIII CAC_nnn'GTG
Cuts at: 0 41 288 420 891
Size: 41 247 132 471
Fragments arranged by size:
471 247 132 41
EaeI y'GGCC_r
Cuts at: 0 357 449 806 891
Size: 357 92 357 85
Fragments arranged by size:
357 357 92 85
EagI C'GGCC_G
Cuts at: 0 449 891
Size: 449 442
EarI CTCTTCn'nnn_
Cuts at: 0 179 674 891
Size: 179 495 217
Fragments arranged by size:
495 217 179
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 64 891
Size: 64 827
EcoICRI GAG'CTC
Cuts at: 0 684 891
Size: 684 207
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 526 891
Size: 424 102 365
Fragments arranged by size:
424 365 102
EcoNI CCTnn'n_nnAGG
Cuts at: 0 652 891
Size: 652 239
EcoO109I rG'GnC_Cy
Cuts at: 0 76 672 846 891
Size: 76 596 174 45
Fragments arranged by size:
596 174 76 45
HaeII r_GCGC'y
Cuts at: 0 28 153 167 891
Size: 28 125 14 724
Fragments arranged by size:
724 125 28 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 187 219 532 564 891
Size: 187 32 313 32 327
Fragments arranged by size:
327 313 187 32 32
HincII GTy'rAC
Cuts at: 0 627 891
Size: 627 264
KasI G'GCGC_C
Cuts at: 0 163 891
Size: 163 728
MluI A'CGCG_T
Cuts at: 0 98 891
Size: 98 793
MscI TGG'CCA
Cuts at: 0 359 891
Size: 359 532
MslI CAynn'nnrTG
Cuts at: 0 283 614 891
Size: 283 331 277
Fragments arranged by size:
331 283 277
MspA1I CmG'CkG
Cuts at: 0 534 891
Size: 534 357
NarI GG'CG_CC
Cuts at: 0 164 891
Size: 164 727
NspI r_CATG'y
Cuts at: 0 633 891
Size: 633 258
PasI CC'CwG_GG
Cuts at: 0 870 891
Size: 870 21
PflMI CCAn_nnn'nTGG
Cuts at: 0 41 537 891
Size: 41 496 354
Fragments arranged by size:
496 354 41
PmlI CAC'GTG
Cuts at: 0 285 740 768 891
Size: 285 455 28 123
Fragments arranged by size:
455 285 123 28
PspOMI G'GGCC_C
Cuts at: 0 76 672 845 891
Size: 76 596 173 46
Fragments arranged by size:
596 173 76 46
PspXI vC'TCGA_Gb
Cuts at: 0 145 891
Size: 145 746
PvuII CAG'CTG
Cuts at: 0 534 891
Size: 534 357
SacI G_AGCT'C
Cuts at: 0 686 891
Size: 686 205
SapI GCTCTTCn'nnn_
Cuts at: 0 674 891
Size: 674 217
SfoI GGC'GCC
Cuts at: 0 165 891
Size: 165 726
SmaI CCC'GGG
Cuts at: 0 331 891
Size: 331 560
SmlI C'TyrA_G
Cuts at: 0 145 484 551 891
Size: 145 339 67 340
Fragments arranged by size:
340 339 145 67
StyI C'CwwG_G
Cuts at: 0 773 891
Size: 773 118
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 333 365 891
Size: 333 32 526
Fragments arranged by size:
526 333 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 42 891
Size: 42 849
XhoI C'TCGA_G
Cuts at: 0 145 891
Size: 145 746
XmaI C'CCGG_G
Cuts at: 0 329 891
Size: 329 562
Enzymes that do cut:
AarI AcuI AflIII AhdI AleI AlwNI ApaI AvaI
BanI BanII BbeI BbsI BglI Bme1580I BpmI BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BstXI BstYI
BtgI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I HaeII Hin4I HincII KasI MluI MscI
MslI MspA1I NarI NspI PasI PflMI PmlI PspOMI
PspXI PvuII SacI SapI SfoI SmaI SmlI StyI
TstI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
BmgBI BmrI BmtI BplI Bpu10I BsaBI BseRI BsgI
BsiWI BsmI BsmBI BspHI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DrdI EcoRI EcoRV FalI FseI FspI FspAI
HindIII HpaI KpnI MfeI MmeI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PfoI
PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI
RsrII SacII SalI SanDI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TatI Tth111I XbaI XmnI ZraI