Restriction map for
pF1KE0544
(Linear) (Six-base) MAPSORT of: KIEE0544.seq Check: 662 from: 1 to: 1281
KIEE0544.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 993 1281
Size: 993 288
AccI GT'mk_AC
Cuts at: 0 728 1281
Size: 728 553
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 249 637 1096 1281
Size: 249 388 459 185
Fragments arranged by size:
459 388 249 185
AfeI AGC'GCT
Cuts at: 0 622 1281
Size: 622 659
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1241 1281
Size: 1241 40
AlwNI CAG_nnn'CTG
Cuts at: 0 876 958 1281
Size: 876 82 323
Fragments arranged by size:
876 323 82
ApaI G_GGCC'C
Cuts at: 0 48 1281
Size: 48 1233
AvaI C'yCGr_G
Cuts at: 0 706 1281
Size: 706 575
AvrII C'CTAG_G
Cuts at: 0 252 1281
Size: 252 1029
BanI G'GyrC_C
Cuts at: 0 5 930 1281
Size: 5 925 351
Fragments arranged by size:
925 351 5
BanII G_rGCy'C
Cuts at: 0 48 1281
Size: 48 1233
BbsI GAAGACnn'nnnn_
Cuts at: 0 212 1281
Size: 212 1069
BbvCI CC'TCA_GC
Cuts at: 0 1264 1281
Size: 1264 17
BclI T'GATC_A
Cuts at: 0 432 1281
Size: 432 849
BglII A'GATC_T
Cuts at: 0 686 1281
Size: 686 595
Bme1580I G_kGCm'C
Cuts at: 0 48 1281
Size: 48 1233
BmrI ACTGGGnnnn_n'
Cuts at: 0 796 1281
Size: 796 485
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 165 424 1281
Size: 160 5 259 857
Fragments arranged by size:
857 259 160 5
Bpu10I CC'TnA_GC
Cuts at: 0 1264 1281
Size: 1264 17
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 787 1281
Size: 211 576 494
Fragments arranged by size:
576 494 211
BsaI GGTCTCn'nnnn_
Cuts at: 0 387 955 1190 1281
Size: 387 568 235 91
Fragments arranged by size:
568 387 235 91
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1129 1159 1281
Size: 1129 30 122
Fragments arranged by size:
1129 122 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 697 1281
Size: 697 584
BseYI C'CCAG_C
Cuts at: 0 154 193 869 1281
Size: 154 39 676 412
Fragments arranged by size:
676 412 154 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 1281
Size: 202 1079
BsiEI CG_ry'CG
Cuts at: 0 1166 1281
Size: 1166 115
BsiHKAI G_wGCw'C
Cuts at: 0 146 1281
Size: 146 1135
BsmI GAATG_Cn'
Cuts at: 0 479 604 858 1281
Size: 479 125 254 423
Fragments arranged by size:
479 423 254 125
Bsp1286I G_dGCh'C
Cuts at: 0 48 146 1281
Size: 48 98 1135
Fragments arranged by size:
1135 98 48
BspHI T'CATG_A
Cuts at: 0 758 860 1281
Size: 758 102 421
Fragments arranged by size:
758 421 102
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 224 332 993 1281
Size: 224 108 661 288
Fragments arranged by size:
661 288 224 108
BsrFI r'CCGG_y
Cuts at: 0 128 1281
Size: 128 1153
BssSI C'ACGA_G
Cuts at: 0 935 1281
Size: 935 346
BstEII G'GTnAC_C
Cuts at: 0 235 1281
Size: 235 1046
BstYI r'GATC_y
Cuts at: 0 686 1281
Size: 686 595
BtgI C'CryG_G
Cuts at: 0 302 590 659 1061 1281
Size: 302 288 69 402 220
Fragments arranged by size:
402 302 288 220 69
BtsI GCAGTG_nn'
Cuts at: 0 250 1281
Size: 250 1031
DrdI GACnn_nn'nnGTC
Cuts at: 0 379 1281
Size: 379 902
EaeI y'GGCC_r
Cuts at: 0 662 824 1049 1163 1281
Size: 662 162 225 114 118
Fragments arranged by size:
662 225 162 118 114
EagI C'GGCC_G
Cuts at: 0 1163 1281
Size: 1163 118
EarI CTCTTCn'nnn_
Cuts at: 0 28 409 1281
Size: 28 381 872
Fragments arranged by size:
872 381 28
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 165 249 424 637 1096 1281
Size: 160 5 84 175 213 459 185
Fragments arranged by size:
459 213 185 175 160 84 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1078 1281
Size: 1078 203
EcoO109I rG'GnC_Cy
Cuts at: 0 14 36 44 45 1132 1281
Size: 14 22 8 1 1087 149
Fragments arranged by size:
1087 149 22 14 8 1
HaeII r_GCGC'y
Cuts at: 0 624 1281
Size: 624 657
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 638 670 671 703 954 986 1022 1054
Size: 638 32 1 32 251 32 36 32
Cuts at: 1054 1281
Size: 227
Fragments arranged by size:
638 251 227 36 32 32 32 32
1
HindIII A'AGCT_T
Cuts at: 0 229 1281
Size: 229 1052
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 926 1281
Size: 622 304 355
Fragments arranged by size:
622 355 304
MscI TGG'CCA
Cuts at: 0 664 826 1281
Size: 664 162 455
Fragments arranged by size:
664 455 162
MslI CAynn'nnrTG
Cuts at: 0 55 163 1281
Size: 55 108 1118
Fragments arranged by size:
1118 108 55
MspA1I CmG'CkG
Cuts at: 0 312 873 955 1281
Size: 312 561 82 326
Fragments arranged by size:
561 326 312 82
NcoI C'CATG_G
Cuts at: 0 302 590 659 1281
Size: 302 288 69 622
Fragments arranged by size:
622 302 288 69
NdeI CA'TA_TG
Cuts at: 0 1107 1281
Size: 1107 174
NspI r_CATG'y
Cuts at: 0 800 1281
Size: 800 481
PflMI CCAn_nnn'nTGG
Cuts at: 0 576 1281
Size: 576 705
PfoI T'CCnGG_A
Cuts at: 0 401 1281
Size: 401 880
PpuMI rG'GwC_Cy
Cuts at: 0 36 1281
Size: 36 1245
PspOMI G'GGCC_C
Cuts at: 0 44 1281
Size: 44 1237
PstI C_TGCA'G
Cuts at: 0 191 836 1004 1281
Size: 191 645 168 277
Fragments arranged by size:
645 277 191 168
PvuII CAG'CTG
Cuts at: 0 312 873 955 1281
Size: 312 561 82 326
Fragments arranged by size:
561 326 312 82
SfcI C'TryA_G
Cuts at: 0 187 729 832 1000 1233 1281
Size: 187 542 103 168 233 48
Fragments arranged by size:
542 233 187 168 103 48
SmlI C'TyrA_G
Cuts at: 0 226 802 1281
Size: 226 576 479
Fragments arranged by size:
576 479 226
StuI AGG'CCT
Cuts at: 0 1120 1281
Size: 1120 161
StyI C'CwwG_G
Cuts at: 0 9 118 200 252 302 590 659 1241
Size: 9 109 82 52 50 288 69 582
Cuts at: 1241 1281
Size: 40
Fragments arranged by size:
582 288 109 82 69 52 50 40
9
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 162 309 597 1044 1281
Size: 162 147 288 447 237
Fragments arranged by size:
447 288 237 162 147
Enzymes that do cut:
AarI AccI AcuI AfeI AlfI AlwNI ApaI AvaI
AvrII BanI BanII BbsI BbvCI BclI BglII Bme1580I
BmrI BpmI Bpu10I BpuEI BsaI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrFI
BssSI BstEII BstYI BtgI BtsI DrdI EaeI EagI
EarI Eco57MI EcoNI EcoO109I HaeII Hin4I HindIII MmeI
MscI MslI MspA1I NcoI NdeI NspI PflMI PfoI
PpuMI PspOMI PstI PvuII SfcI SmlI StuI StyI
XcmI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AleI
AloI ApaLI ApoI AscI AseI AsiSI BaeI BamHI
BbeI BcgI BciVI BfrBI BglI BlpI BmgBI BmtI
BplI BsaAI BsaBI BsaHI BsaWI BsiWI BsmBI BspEI
BsrBI BsrDI BsrGI BssHII BstAPI BstBI BstXI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII EciI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HincII HpaI
KasI KpnI MfeI MluI NaeI NarI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI