(Linear) (Six-base) MAPSORT of: KIEE0518.seq  Check: 1942  from: 1  to: 498



KIEE0518.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:15  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     227     498 
   Size:        227     271
 
AflIII A'CryG_T

Cuts at:      0      47     498 
   Size:         47     451
 
AleI CACnn'nnGTG

Cuts at:      0      52     498 
   Size:         52     446
 
BanI G'GyrC_C

Cuts at:      0     195     498 
   Size:        195     303
 
BglI GCCn_nnn'nGGC

Cuts at:      0     277     498 
   Size:        277     221
 
BlpI GC'TnA_GC

Cuts at:      0     127     498 
   Size:        127     371
 
Bme1580I G_kGCm'C

Cuts at:      0     200     337     498 
   Size:        200     137     161

  Fragments arranged by size: 

                 200     161     137
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     209     435     498 
   Size:        209     226      63

  Fragments arranged by size: 

                 226     209      63
 
BsaAI yAC'GTr

Cuts at:      0      50     153     498 
   Size:         50     103     345

  Fragments arranged by size: 

                 345     103      50
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     483     498 
   Size:        483      15
 
BseYI C'CCAG_C

Cuts at:      0     199     487     498 
   Size:        199     288      11

  Fragments arranged by size: 

                 288     199      11
 
Bsp1286I G_dGCh'C

Cuts at:      0     200     337     498 
   Size:        200     137     161

  Fragments arranged by size: 

                 200     161     137
 
BsrBI CCG'CTC

Cuts at:      0      98     498 
   Size:         98     400
 
BstEII G'GTnAC_C

Cuts at:      0     228     498 
   Size:        228     270
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      81     498 
   Size:         81     417
 
BtgI C'CryG_G

Cuts at:      0      16     233     498 
   Size:         16     217     265

  Fragments arranged by size: 

                 265     217      16
 
BtsI GCAGTG_nn'

Cuts at:      0     335     498 
   Size:        335     163
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     366     401     498 
   Size:        366      35      97

  Fragments arranged by size: 

                 366      97      35
 
EaeI y'GGCC_r

Cuts at:      0     140     278     498 
   Size:        140     138     220

  Fragments arranged by size: 

                 220     140     138
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     175     498 
   Size:        175     323
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     209     227     435     498 
   Size:        209      18     208      63

  Fragments arranged by size: 

                 209     208      63      18
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     209     498 
   Size:        209     289
 
FspI TGC'GCA

Cuts at:      0     460     498 
   Size:        460      38
 
HaeII r_GCGC'y

Cuts at:      0     224     498 
   Size:        224     274
 
HincII GTy'rAC

Cuts at:      0     395     498 
   Size:        395     103
 
MscI TGG'CCA

Cuts at:      0     142     280     498 
   Size:        142     138     218

  Fragments arranged by size: 

                 218     142     138
 
MslI CAynn'nnrTG

Cuts at:      0      52     498 
   Size:         52     446
 
MspA1I CmG'CkG

Cuts at:      0      18     112     347     498 
   Size:         18      94     235     151

  Fragments arranged by size: 

                 235     151      94      18
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     347     498 
   Size:        347     151
 
PfoI T'CCnGG_A

Cuts at:      0     443     498 
   Size:        443      55
 
PmlI CAC'GTG

Cuts at:      0      50     498 
   Size:         50     448
 
SacII CC_GC'GG

Cuts at:      0      19     498 
   Size:         19     479
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      42     498 
   Size:         42     456
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     231     498 
   Size:        231     267
 

 Enzymes that do cut:

     AcuI   AflIII     AleI     BanI     BglI     BlpI Bme1580I     BpmI
    BsaAI    BseRI    BseYI Bsp1286I    BsrBI   BstEII    BstXI     BtgI
     BtsI    CspCI     EaeI     EciI  Eco57MI    EcoNI     FspI    HaeII
   HincII     MscI     MslI   MspA1I    PflMI     PfoI     PmlI    SacII
    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII    BmgBI
     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI
    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI     DraI
   DraIII     DrdI     EagI     EarI  EcoICRI EcoO109I    EcoRI    EcoRV
     FalI     FseI    FspAI    Hin4I  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI