Restriction map for
pF1KE0511
(Linear) (Six-base) MAPSORT of: KIEE0511.seq Check: 7625 from: 1 to: 927
KIEE0511.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 0 910 927
Size: 910 17
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 551 659 927
Size: 551 108 268
Fragments arranged by size:
551 268 108
AflIII A'CryG_T
Cuts at: 0 765 927
Size: 765 162
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 53 723 927
Size: 53 670 204
Fragments arranged by size:
670 204 53
ApoI r'AATT_y
Cuts at: 0 133 296 445 747 927
Size: 133 163 149 302 180
Fragments arranged by size:
302 180 163 149 133
AseI AT'TA_AT
Cuts at: 0 596 927
Size: 596 331
BamHI G'GATC_C
Cuts at: 0 312 927
Size: 312 615
BanI G'GyrC_C
Cuts at: 0 89 927
Size: 89 838
BbsI GAAGACnn'nnnn_
Cuts at: 0 344 927
Size: 344 583
BbvCI CC'TCA_GC
Cuts at: 0 149 927
Size: 149 778
BciVI GTATCCnnnnn_n'
Cuts at: 0 397 927
Size: 397 530
BclI T'GATC_A
Cuts at: 0 621 927
Size: 621 306
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 735 927
Size: 735 192
Bpu10I CC'TnA_GC
Cuts at: 0 149 927
Size: 149 778
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 927
Size: 367 560
BsaBI GATnn'nnATC
Cuts at: 0 101 471 927
Size: 101 370 456
Fragments arranged by size:
456 370 101
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 397 427 927
Size: 397 30 500
Fragments arranged by size:
500 397 30
BsmI GAATG_Cn'
Cuts at: 0 712 927
Size: 712 215
BspHI T'CATG_A
Cuts at: 0 303 927
Size: 303 624
BstEII G'GTnAC_C
Cuts at: 0 816 927
Size: 816 111
BstYI r'GATC_y
Cuts at: 0 312 831 927
Size: 312 519 96
Fragments arranged by size:
519 312 96
Bsu36I CC'TnA_GG
Cuts at: 0 47 927
Size: 47 880
BtsI GCAGTG_nn'
Cuts at: 0 416 927
Size: 416 511
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 846 881 927
Size: 846 35 46
Fragments arranged by size:
846 46 35
DraI TTT'AAA
Cuts at: 0 291 927
Size: 291 636
EaeI y'GGCC_r
Cuts at: 0 680 927
Size: 680 247
EarI CTCTTCn'nnn_
Cuts at: 0 519 683 927
Size: 519 164 244
Fragments arranged by size:
519 244 164
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 551 659 735 927
Size: 551 108 76 192
Fragments arranged by size:
551 192 108 76
EcoO109I rG'GnC_Cy
Cuts at: 0 351 927
Size: 351 576
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 56 97 361 927
Size: 56 41 264 566
Fragments arranged by size:
566 264 56 41
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 151 183 927
Size: 151 32 744
Fragments arranged by size:
744 151 32
HincII GTy'rAC
Cuts at: 0 656 927
Size: 656 271
HindIII A'AGCT_T
Cuts at: 0 62 927
Size: 62 865
MscI TGG'CCA
Cuts at: 0 682 927
Size: 682 245
MslI CAynn'nnrTG
Cuts at: 0 84 239 927
Size: 84 155 688
Fragments arranged by size:
688 155 84
MspA1I CmG'CkG
Cuts at: 0 713 927
Size: 713 214
NdeI CA'TA_TG
Cuts at: 0 456 927
Size: 456 471
NspI r_CATG'y
Cuts at: 0 769 927
Size: 769 158
PciI A'CATG_T
Cuts at: 0 765 927
Size: 765 162
PfoI T'CCnGG_A
Cuts at: 0 366 927
Size: 366 561
PpuMI rG'GwC_Cy
Cuts at: 0 351 927
Size: 351 576
PvuII CAG'CTG
Cuts at: 0 713 927
Size: 713 214
SmlI C'TyrA_G
Cuts at: 0 346 927
Size: 346 581
SspI AAT'ATT
Cuts at: 0 594 927
Size: 594 333
StuI AGG'CCT
Cuts at: 0 500 927
Size: 500 427
XmnI GAAnn'nnTTC
Cuts at: 0 299 927
Size: 299 628
Enzymes that do cut:
AccI AcuI AflIII AlfI ApoI AseI BamHI BanI
BbsI BbvCI BciVI BclI BpmI Bpu10I BpuEI BsaBI
BsaXI BsmI BspHI BstEII BstYI Bsu36I BtsI CspCI
DraI EaeI EarI Eco57MI EcoO109I FalI Hin4I HincII
HindIII MscI MslI MspA1I NdeI NspI PciI PfoI
PpuMI PvuII SmlI SspI StuI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI AlwNI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BanII BbeI BcgI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BsaI BsaAI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstXI BstZ17I BtgI BtgZI
ClaI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FseI FspI FspAI HaeII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI PacI PasI PflMI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StyI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI ZraI