Restriction map for
pF1KE0505
(Linear) (Six-base) MAPSORT of: KIEE0505.seq Check: 1420 from: 1 to: 753
KIEE0505.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 408 753
Size: 408 345
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 335 428 753
Size: 238 97 93 325
Fragments arranged by size:
325 238 97 93
AfeI AGC'GCT
Cuts at: 0 253 753
Size: 253 500
AhdI GACnn_n'nnGTC
Cuts at: 0 599 753
Size: 599 154
AleI CACnn'nnGTG
Cuts at: 0 341 434 753
Size: 341 93 319
Fragments arranged by size:
341 319 93
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 347 440 753
Size: 347 93 313
Fragments arranged by size:
347 313 93
ApaI G_GGCC'C
Cuts at: 0 31 753
Size: 31 722
BanII G_rGCy'C
Cuts at: 0 31 753
Size: 31 722
BbsI GAAGACnn'nnnn_
Cuts at: 0 208 542 590 638 753
Size: 208 334 48 48 115
Fragments arranged by size:
334 208 115 48 48
BbvCI CC'TCA_GC
Cuts at: 0 190 753
Size: 190 563
BglII A'GATC_T
Cuts at: 0 504 753
Size: 504 249
Bme1580I G_kGCm'C
Cuts at: 0 31 753
Size: 31 722
Bpu10I CC'TnA_GC
Cuts at: 0 190 753
Size: 190 563
BsaI GGTCTCn'nnnn_
Cuts at: 0 607 707 753
Size: 607 100 46
Fragments arranged by size:
607 100 46
BsaHI Gr'CG_yC
Cuts at: 0 205 239 328 753
Size: 205 34 89 425
Fragments arranged by size:
425 205 89 34
BsaWI w'CCGG_w
Cuts at: 0 645 753
Size: 645 108
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 229 259 753
Size: 229 30 494
Fragments arranged by size:
494 229 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 364 457 753
Size: 364 93 296
Fragments arranged by size:
364 296 93
BseYI C'CCAG_C
Cuts at: 0 31 683 753
Size: 31 652 70
Fragments arranged by size:
652 70 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 317 343 410 436 753
Size: 317 26 67 26 317
Fragments arranged by size:
317 317 67 26 26
BsmBI CGTCTCn'nnnn_
Cuts at: 0 267 753
Size: 267 486
Bsp1286I G_dGCh'C
Cuts at: 0 31 753
Size: 31 722
BspHI T'CATG_A
Cuts at: 0 542 590 638 753
Size: 542 48 48 115
Fragments arranged by size:
542 115 48 48
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 408 753
Size: 408 345
BsrBI CCG'CTC
Cuts at: 0 245 753
Size: 245 508
BstAPI GCAn_nnn'nTGC
Cuts at: 0 142 753
Size: 142 611
BstXI CCAn_nnnn'nTGG
Cuts at: 0 234 753
Size: 234 519
BstYI r'GATC_y
Cuts at: 0 504 552 753
Size: 504 48 201
Fragments arranged by size:
504 201 48
Bsu36I CC'TnA_GG
Cuts at: 0 380 753
Size: 380 373
BtsI GCAGTG_nn'
Cuts at: 0 131 753
Size: 131 622
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 335 428 753
Size: 238 97 93 325
Fragments arranged by size:
325 238 97 93
EcoO109I rG'GnC_Cy
Cuts at: 0 28 39 73 307 753
Size: 28 11 34 234 446
Fragments arranged by size:
446 234 34 28 11
HaeII r_GCGC'y
Cuts at: 0 255 753
Size: 255 498
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 229 261 276 308 537 569 585 617
Size: 229 32 15 32 229 32 16 32
Cuts at: 617 753
Size: 136
Fragments arranged by size:
229 229 136 32 32 32 32 16
15
MslI CAynn'nnrTG
Cuts at: 0 86 341 434 753
Size: 86 255 93 319
Fragments arranged by size:
319 255 93 86
MspA1I CmG'CkG
Cuts at: 0 35 753
Size: 35 718
PflMI CCAn_nnn'nTGG
Cuts at: 0 254 495 753
Size: 254 241 258
Fragments arranged by size:
258 254 241
PpuMI rG'GwC_Cy
Cuts at: 0 73 307 753
Size: 73 234 446
Fragments arranged by size:
446 234 73
PshAI GACnn'nnGTC
Cuts at: 0 398 753
Size: 398 355
PspOMI G'GGCC_C
Cuts at: 0 27 753
Size: 27 726
PstI C_TGCA'G
Cuts at: 0 61 753
Size: 61 692
SanDI GG'GwC_CC
Cuts at: 0 73 753
Size: 73 680
SfcI C'TryA_G
Cuts at: 0 57 753
Size: 57 696
XbaI T'CTAG_A
Cuts at: 0 294 753
Size: 294 459
Enzymes that do cut:
AarI AcuI AfeI AhdI AleI AlfI ApaI BanII
BbsI BbvCI BglII Bme1580I Bpu10I BsaI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsmBI Bsp1286I BspHI BspMI
BsrBI BstAPI BstXI BstYI Bsu36I BtsI Eco57MI EcoO109I
HaeII Hin4I MslI MspA1I PflMI PpuMI PshAI PspOMI
PstI SanDI SfcI XbaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AloI
AlwNI ApaLI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BcgI BciVI BclI BfrBI
BglI BlpI BmgBI BmrI BmtI BplI BpmI BpuEI
BsaAI BsaBI BsiEI BsiHKAI BsiWI BsmI BspEI BsrDI
BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgI
BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PfoI PmeI PmlI PpiI PsiI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI SwaI TaqII
TatI TstI Tth111I XcmI XhoI XmaI XmnI ZraI