Restriction map for
pF1KE0502
(Linear) (Six-base) MAPSORT of: KIEE0502.seq Check: 681 from: 1 to: 633
KIEE0502.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AflIII A'CryG_T
Cuts at: 0 512 633
Size: 512 121
BanI G'GyrC_C
Cuts at: 0 503 633
Size: 503 130
BanII G_rGCy'C
Cuts at: 0 122 172 423 633
Size: 122 50 251 210
Fragments arranged by size:
251 210 122 50
BbeI G_GCGC'C
Cuts at: 0 507 633
Size: 507 126
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 13 47 633
Size: 13 34 586
Fragments arranged by size:
586 34 13
Bme1580I G_kGCm'C
Cuts at: 0 136 410 633
Size: 136 274 223
Fragments arranged by size:
274 223 136
BmgBI CAC'GTC
Cuts at: 0 405 470 633
Size: 405 65 163
Fragments arranged by size:
405 163 65
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 262 633
Size: 178 84 371
Fragments arranged by size:
371 178 84
BsaI GGTCTCn'nnnn_
Cuts at: 0 47 633
Size: 47 586
BsaAI yAC'GTr
Cuts at: 0 513 633
Size: 513 120
BsaHI Gr'CG_yC
Cuts at: 0 504 554 633
Size: 504 50 79
Fragments arranged by size:
504 79 50
BseYI C'CCAG_C
Cuts at: 0 136 263 363 409 625 633
Size: 136 127 100 46 216 8
Fragments arranged by size:
216 136 127 100 46 8
BsiEI CG_ry'CG
Cuts at: 0 128 633
Size: 128 505
BsiHKAI G_wGCw'C
Cuts at: 0 21 122 633
Size: 21 101 511
Fragments arranged by size:
511 101 21
Bsp1286I G_dGCh'C
Cuts at: 0 21 122 136 172 410 423 633
Size: 21 101 14 36 238 13 210
Fragments arranged by size:
238 210 101 36 21 14 13
BsrBI CCG'CTC
Cuts at: 0 522 633
Size: 522 111
BstAPI GCAn_nnn'nTGC
Cuts at: 0 113 579 633
Size: 113 466 54
Fragments arranged by size:
466 113 54
BstXI CCAn_nnnn'nTGG
Cuts at: 0 180 633
Size: 180 453
BtgI C'CryG_G
Cuts at: 0 48 193 222 498 633
Size: 48 145 29 276 135
Fragments arranged by size:
276 145 135 48 29
EaeI y'GGCC_r
Cuts at: 0 125 375 633
Size: 125 250 258
Fragments arranged by size:
258 250 125
EagI C'GGCC_G
Cuts at: 0 125 633
Size: 125 508
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 633
Size: 19 614
EcoICRI GAG'CTC
Cuts at: 0 120 633
Size: 120 513
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 262 633
Size: 178 84 371
Fragments arranged by size:
371 178 84
EcoO109I rG'GnC_Cy
Cuts at: 0 215 633
Size: 215 418
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 96 633
Size: 96 537
HaeII r_GCGC'y
Cuts at: 0 507 633
Size: 507 126
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 259 291 633
Size: 259 32 342
Fragments arranged by size:
342 259 32
KasI G'GCGC_C
Cuts at: 0 503 633
Size: 503 130
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 302 633
Size: 302 331
MslI CAynn'nnrTG
Cuts at: 0 178 542 633
Size: 178 364 91
Fragments arranged by size:
364 178 91
MspA1I CmG'CkG
Cuts at: 0 37 224 570 633
Size: 37 187 346 63
Fragments arranged by size:
346 187 63 37
NarI GG'CG_CC
Cuts at: 0 504 633
Size: 504 129
NcoI C'CATG_G
Cuts at: 0 48 633
Size: 48 585
PflMI CCAn_nnn'nTGG
Cuts at: 0 416 633
Size: 416 217
PfoI T'CCnGG_A
Cuts at: 0 431 633
Size: 431 202
PstI C_TGCA'G
Cuts at: 0 36 575 633
Size: 36 539 58
Fragments arranged by size:
539 58 36
PvuII CAG'CTG
Cuts at: 0 37 570 633
Size: 37 533 63
Fragments arranged by size:
533 63 37
SacI G_AGCT'C
Cuts at: 0 122 633
Size: 122 511
SacII CC_GC'GG
Cuts at: 0 225 633
Size: 225 408
SfcI C'TryA_G
Cuts at: 0 32 571 633
Size: 32 539 62
Fragments arranged by size:
539 62 32
SfoI GGC'GCC
Cuts at: 0 505 633
Size: 505 128
SspI AAT'ATT
Cuts at: 0 324 633
Size: 324 309
StyI C'CwwG_G
Cuts at: 0 48 57 102 633
Size: 48 9 45 531
Fragments arranged by size:
531 48 45 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 160 633
Size: 160 473
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 55 633
Size: 55 578
Enzymes that do cut:
AflIII BanI BanII BbeI BcgI Bme1580I BmgBI BpmI
BsaI BsaAI BsaHI BseYI BsiEI BsiHKAI Bsp1286I BsrBI
BstAPI BstXI BtgI EaeI EagI EciI EcoICRI Eco57MI
EcoO109I FalI HaeII Hin4I KasI MmeI MslI MspA1I
NarI NcoI PflMI PfoI PstI PvuII SacI SacII
SfcI SfoI SspI StyI TaqII XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BbsI BbvCI BciVI BclI BfrBI BglI BglII BlpI
BmrI BmtI BplI Bpu10I BpuEI BsaBI BsaWI BsaXI
BseRI BsgI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstYI
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EarI EcoNI EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MscI NaeI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI StuI SwaI TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI