Restriction map for
pF1KE0495
(Linear) (Six-base) MAPSORT of: KIEE0495.seq Check: 9055 from: 1 to: 621
KIEE0495.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AvaI C'yCGr_G
Cuts at: 0 345 621
Size: 345 276
BamHI G'GATC_C
Cuts at: 0 611 621
Size: 611 10
BanII G_rGCy'C
Cuts at: 0 574 621
Size: 574 47
BlpI GC'TnA_GC
Cuts at: 0 397 621
Size: 397 224
Bme1580I G_kGCm'C
Cuts at: 0 133 621
Size: 133 488
BmgBI CAC'GTC
Cuts at: 0 462 621
Size: 462 159
BmrI ACTGGGnnnn_n'
Cuts at: 0 483 621
Size: 483 138
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 588 621
Size: 588 33
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 403 621
Size: 163 240 218
Fragments arranged by size:
240 218 163
BsaBI GATnn'nnATC
Cuts at: 0 407 621
Size: 407 214
BsaWI w'CCGG_w
Cuts at: 0 541 621
Size: 541 80
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 179 209 621
Size: 179 30 412
Fragments arranged by size:
412 179 30
BseYI C'CCAG_C
Cuts at: 0 133 260 621
Size: 133 127 361
Fragments arranged by size:
361 133 127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 616 621
Size: 616 5
BsiEI CG_ry'CG
Cuts at: 0 125 621
Size: 125 496
BsmI GAATG_Cn'
Cuts at: 0 532 621
Size: 532 89
Bsp1286I G_dGCh'C
Cuts at: 0 133 574 621
Size: 133 441 47
Fragments arranged by size:
441 133 47
BsrDI GCAATG_nn'
Cuts at: 0 49 447 621
Size: 49 398 174
Fragments arranged by size:
398 174 49
BsrFI r'CCGG_y
Cuts at: 0 104 621
Size: 104 517
BssSI C'ACGA_G
Cuts at: 0 112 621
Size: 112 509
BstXI CCAn_nnnn'nTGG
Cuts at: 0 141 621
Size: 141 480
BstYI r'GATC_y
Cuts at: 0 611 621
Size: 611 10
BtgI C'CryG_G
Cuts at: 0 425 621
Size: 425 196
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 416 621
Size: 416 205
EaeI y'GGCC_r
Cuts at: 0 122 621
Size: 122 499
EagI C'GGCC_G
Cuts at: 0 122 621
Size: 122 499
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 403 621
Size: 163 240 218
Fragments arranged by size:
240 218 163
EcoO109I rG'GnC_Cy
Cuts at: 0 61 452 473 621
Size: 61 391 21 148
Fragments arranged by size:
391 148 61 21
MslI CAynn'nnrTG
Cuts at: 0 542 621
Size: 542 79
MspA1I CmG'CkG
Cuts at: 0 58 621
Size: 58 563
NaeI GCC'GGC
Cuts at: 0 106 621
Size: 106 515
NcoI C'CATG_G
Cuts at: 0 425 621
Size: 425 196
NgoMIV G'CCGG_C
Cuts at: 0 104 621
Size: 104 517
NspI r_CATG'y
Cuts at: 0 211 621
Size: 211 410
PasI CC'CwG_GG
Cuts at: 0 7 621
Size: 7 614
PflMI CCAn_nnn'nTGG
Cuts at: 0 426 431 621
Size: 426 5 190
Fragments arranged by size:
426 190 5
PfoI T'CCnGG_A
Cuts at: 0 560 621
Size: 560 61
PpuMI rG'GwC_Cy
Cuts at: 0 61 452 621
Size: 61 391 169
Fragments arranged by size:
391 169 61
PspXI vC'TCGA_Gb
Cuts at: 0 345 621
Size: 345 276
SanDI GG'GwC_CC
Cuts at: 0 61 621
Size: 61 560
SmlI C'TyrA_G
Cuts at: 0 345 621
Size: 345 276
SphI G_CATG'C
Cuts at: 0 211 621
Size: 211 410
StyI C'CwwG_G
Cuts at: 0 219 425 621
Size: 219 206 196
Fragments arranged by size:
219 206 196
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 183 621
Size: 183 438
TatI w'GTAC_w
Cuts at: 0 230 621
Size: 230 391
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 179 211 621
Size: 179 32 410
Fragments arranged by size:
410 179 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 466 621
Size: 466 155
XhoI C'TCGA_G
Cuts at: 0 345 621
Size: 345 276
Enzymes that do cut:
AvaI BamHI BanII BlpI Bme1580I BmgBI BmrI BplI
BpmI BsaBI BsaWI BsaXI BseYI BsgI BsiEI BsmI
Bsp1286I BsrDI BsrFI BssSI BstXI BstYI BtgI BtgZI
EaeI EagI Eco57MI EcoO109I MslI MspA1I NaeI NcoI
NgoMIV NspI PasI PflMI PfoI PpuMI PspXI SanDI
SmlI SphI StyI TaqII TatI TstI XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BanI
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BmtI Bpu10I BpuEI BsaI BsaAI BsaHI BseRI
BsiHKAI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrGI
BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtsI ClaI
CspCI DraI DraIII DrdI EarI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI NarI NdeI NheI NotI NruI NsiI PacI
PciI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI StuI SwaI Tth111I
XbaI XmaI XmnI ZraI