Restriction map for
pF1KE0487
(Linear) (Six-base) MAPSORT of: KIEE0487.seq Check: 6140 from: 1 to: 1071
KIEE0487.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AatII G_ACGT'C
Cuts at: 0 221 629 1071
Size: 221 408 442
Fragments arranged by size:
442 408 221
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 811 1071
Size: 811 260
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 920 1071
Size: 920 151
AlwNI CAG_nnn'CTG
Cuts at: 0 87 940 1051 1071
Size: 87 853 111 20
Fragments arranged by size:
853 111 87 20
BanI G'GyrC_C
Cuts at: 0 421 1071
Size: 421 650
BbeI G_GCGC'C
Cuts at: 0 425 1071
Size: 425 646
BbsI GAAGACnn'nnnn_
Cuts at: 0 809 1071
Size: 809 262
BbvCI CC'TCA_GC
Cuts at: 0 403 1071
Size: 403 668
BciVI GTATCCnnnnn_n'
Cuts at: 0 867 1071
Size: 867 204
BclI T'GATC_A
Cuts at: 0 717 1071
Size: 717 354
BfrBI ATG'CAT
Cuts at: 0 976 1071
Size: 976 95
BglII A'GATC_T
Cuts at: 0 914 1071
Size: 914 157
Bme1580I G_kGCm'C
Cuts at: 0 545 1071
Size: 545 526
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 382 478 643 1071
Size: 217 165 96 165 428
Fragments arranged by size:
428 217 165 165 96
Bpu10I CC'TnA_GC
Cuts at: 0 403 1071
Size: 403 668
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 617 1071
Size: 617 454
BsaI GGTCTCn'nnnn_
Cuts at: 0 700 1071
Size: 700 371
BsaBI GATnn'nnATC
Cuts at: 0 941 1071
Size: 941 130
BsaHI Gr'CG_yC
Cuts at: 0 110 218 422 557 626 1071
Size: 110 108 204 135 69 445
Fragments arranged by size:
445 204 135 110 108 69
BsaWI w'CCGG_w
Cuts at: 0 68 230 284 374 1071
Size: 68 162 54 90 697
Fragments arranged by size:
697 162 90 68 54
BseYI C'CCAG_C
Cuts at: 0 319 1071
Size: 319 752
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 823 1071
Size: 190 633 248
Fragments arranged by size:
633 248 190
BsiHKAI G_wGCw'C
Cuts at: 0 776 1071
Size: 776 295
BsmBI CGTCTCn'nnnn_
Cuts at: 0 209 1071
Size: 209 862
Bsp1286I G_dGCh'C
Cuts at: 0 545 776 1071
Size: 545 231 295
Fragments arranged by size:
545 295 231
BspEI T'CCGG_A
Cuts at: 0 68 230 284 374 1071
Size: 68 162 54 90 697
Fragments arranged by size:
697 162 90 68 54
BspHI T'CATG_A
Cuts at: 0 714 1071
Size: 714 357
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 461 1071
Size: 461 610
BsrFI r'CCGG_y
Cuts at: 0 470 1071
Size: 470 601
BssSI C'ACGA_G
Cuts at: 0 150 1071
Size: 150 921
BstAPI GCAn_nnn'nTGC
Cuts at: 0 780 1071
Size: 780 291
BstXI CCAn_nnnn'nTGG
Cuts at: 0 966 1071
Size: 966 105
BstYI r'GATC_y
Cuts at: 0 914 1071
Size: 914 157
BtgI C'CryG_G
Cuts at: 0 425 488 1071
Size: 425 63 583
Fragments arranged by size:
583 425 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 323 1071
Size: 323 748
BtsI GCAGTG_nn'
Cuts at: 0 128 1071
Size: 128 943
DraI TTT'AAA
Cuts at: 0 56 1071
Size: 56 1015
DrdI GACnn_nn'nnGTC
Cuts at: 0 643 1071
Size: 643 428
EaeI y'GGCC_r
Cuts at: 0 468 1071
Size: 468 603
EarI CTCTTCn'nnn_
Cuts at: 0 1001 1071
Size: 1001 70
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 382 478 643 811 1071
Size: 217 165 96 165 168 260
Fragments arranged by size:
260 217 168 165 165 96
EcoNI CCTnn'n_nnAGG
Cuts at: 0 302 343 457 1071
Size: 302 41 114 614
Fragments arranged by size:
614 302 114 41
EcoO109I rG'GnC_Cy
Cuts at: 0 255 522 732 1071
Size: 255 267 210 339
Fragments arranged by size:
339 267 255 210
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 339 1071
Size: 339 732
HaeII r_GCGC'y
Cuts at: 0 425 1071
Size: 425 646
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 282 314 1071
Size: 282 32 757
Fragments arranged by size:
757 282 32
KasI G'GCGC_C
Cuts at: 0 421 1071
Size: 421 650
MslI CAynn'nnrTG
Cuts at: 0 784 1071
Size: 784 287
MspA1I CmG'CkG
Cuts at: 0 195 1071
Size: 195 876
NaeI GCC'GGC
Cuts at: 0 472 1071
Size: 472 599
NarI GG'CG_CC
Cuts at: 0 422 1071
Size: 422 649
NcoI C'CATG_G
Cuts at: 0 425 488 1071
Size: 425 63 583
Fragments arranged by size:
583 425 63
NgoMIV G'CCGG_C
Cuts at: 0 470 1071
Size: 470 601
NsiI A_TGCA'T
Cuts at: 0 978 1071
Size: 978 93
NspI r_CATG'y
Cuts at: 0 783 980 1071
Size: 783 197 91
Fragments arranged by size:
783 197 91
PfoI T'CCnGG_A
Cuts at: 0 329 885 1071
Size: 329 556 186
Fragments arranged by size:
556 329 186
PpuMI rG'GwC_Cy
Cuts at: 0 255 522 1071
Size: 255 267 549
Fragments arranged by size:
549 267 255
PshAI GACnn'nnGTC
Cuts at: 0 109 520 1071
Size: 109 411 551
Fragments arranged by size:
551 411 109
PstI C_TGCA'G
Cuts at: 0 189 395 1071
Size: 189 206 676
Fragments arranged by size:
676 206 189
PvuII CAG'CTG
Cuts at: 0 195 1071
Size: 195 876
SfcI C'TryA_G
Cuts at: 0 185 391 1071
Size: 185 206 680
Fragments arranged by size:
680 206 185
SfoI GGC'GCC
Cuts at: 0 423 1071
Size: 423 648
SmlI C'TyrA_G
Cuts at: 0 632 1071
Size: 632 439
SphI G_CATG'C
Cuts at: 0 980 1071
Size: 980 91
StuI AGG'CCT
Cuts at: 0 463 1071
Size: 463 608
StyI C'CwwG_G
Cuts at: 0 425 488 518 1071
Size: 425 63 30 553
Fragments arranged by size:
553 425 63 30
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 495 1071
Size: 495 576
ZraI GAC'GTC
Cuts at: 0 219 627 1071
Size: 219 408 444
Fragments arranged by size:
444 408 219
Enzymes that do cut:
AatII AcuI AlfI AlwNI BanI BbeI BbsI BbvCI
BciVI BclI BfrBI BglII Bme1580I BpmI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaWI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrFI BssSI BstAPI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EarI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KasI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PfoI
PpuMI PshAI PstI PvuII SfcI SfoI SmlI SphI
StuI StyI XcmI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanII BcgI BglI
BlpI BmgBI BmrI BmtI BplI BsaAI BsaXI BseRI
BsiEI BsiWI BsmI BsrBI BsrDI BsrGI BssHII BstBI
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EagI EciI
EcoICRI EcoRI EcoRV FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI NdeI NheI
NotI NruI PacI PasI PciI PflMI PmeI PmlI
PpiI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI