Restriction map for
pF1KE0485
(Linear) (Six-base) MAPSORT of: KIEE0485.seq Check: 1371 from: 1 to: 597
KIEE0485.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 597
Size: 325 272
AflIII A'CryG_T
Cuts at: 0 354 597
Size: 354 243
AleI CACnn'nnGTG
Cuts at: 0 355 493 597
Size: 355 138 104
Fragments arranged by size:
355 138 104
ApaI G_GGCC'C
Cuts at: 0 26 444 597
Size: 26 418 153
Fragments arranged by size:
418 153 26
ApoI r'AATT_y
Cuts at: 0 469 597
Size: 469 128
BamHI G'GATC_C
Cuts at: 0 39 597
Size: 39 558
BanI G'GyrC_C
Cuts at: 0 127 597
Size: 127 470
BanII G_rGCy'C
Cuts at: 0 26 298 444 597
Size: 26 272 146 153
Fragments arranged by size:
272 153 146 26
BglII A'GATC_T
Cuts at: 0 263 597
Size: 263 334
Bme1580I G_kGCm'C
Cuts at: 0 26 444 597
Size: 26 418 153
Fragments arranged by size:
418 153 26
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 312 565 597
Size: 312 253 32
Fragments arranged by size:
312 253 32
BsaAI yAC'GTr
Cuts at: 0 357 597
Size: 357 240
BseYI C'CCAG_C
Cuts at: 0 12 436 482 597
Size: 12 424 46 115
Fragments arranged by size:
424 115 46 12
BsiEI CG_ry'CG
Cuts at: 0 204 597
Size: 204 393
BsiHKAI G_wGCw'C
Cuts at: 0 545 597
Size: 545 52
Bsp1286I G_dGCh'C
Cuts at: 0 26 298 444 545 597
Size: 26 272 146 101 52
Fragments arranged by size:
272 146 101 52 26
BspHI T'CATG_A
Cuts at: 0 455 597
Size: 455 142
BstAPI GCAn_nnn'nTGC
Cuts at: 0 426 597
Size: 426 171
BstYI r'GATC_y
Cuts at: 0 39 263 597
Size: 39 224 334
Fragments arranged by size:
334 224 39
BtsI GCAGTG_nn'
Cuts at: 0 415 477 597
Size: 415 62 120
Fragments arranged by size:
415 120 62
EaeI y'GGCC_r
Cuts at: 0 201 597
Size: 201 396
EagI C'GGCC_G
Cuts at: 0 201 597
Size: 201 396
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 312 325 565 597
Size: 312 13 240 32
Fragments arranged by size:
312 240 32 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 406 597
Size: 406 191
EcoO109I rG'GnC_Cy
Cuts at: 0 440 597
Size: 440 157
HaeII r_GCGC'y
Cuts at: 0 117 597
Size: 117 480
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 353 385 597
Size: 353 32 212
Fragments arranged by size:
353 212 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 230 597
Size: 83 147 367
Fragments arranged by size:
367 147 83
MslI CAynn'nnrTG
Cuts at: 0 355 493 597
Size: 355 138 104
Fragments arranged by size:
355 138 104
PasI CC'CwG_GG
Cuts at: 0 524 597
Size: 524 73
PfoI T'CCnGG_A
Cuts at: 0 289 597
Size: 289 308
PmlI CAC'GTG
Cuts at: 0 357 597
Size: 357 240
PspOMI G'GGCC_C
Cuts at: 0 22 440 597
Size: 22 418 157
Fragments arranged by size:
418 157 22
SexAI A'CCwGG_T
Cuts at: 0 230 597
Size: 230 367
SfcI C'TryA_G
Cuts at: 0 267 597
Size: 267 330
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 123 597
Size: 123 474
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 33 526 556 597
Size: 33 493 30 41
Fragments arranged by size:
493 41 33 30
Enzymes that do cut:
AcuI AflIII AleI ApaI ApoI BamHI BanI BanII
BglII Bme1580I BpmI BsaAI BseYI BsiEI BsiHKAI Bsp1286I
BspHI BstAPI BstYI BtsI EaeI EagI Eco57MI EcoNI
EcoO109I HaeII Hin4I MmeI MslI PasI PfoI PmlI
PspOMI SexAI SfcI TaqII XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI AlwNI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BlpI BmgBI BmrI BmtI BplI
Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI
BsgI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I
Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII DrdI
EarI EciI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MscI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI SwaI TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI