Restriction map for
pF1KE0470
(Linear) (Six-base) MAPSORT of: KIEE0470.seq Check: 4440 from: 1 to: 903
KIEE0470.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
Acc65I G'GTAC_C
Cuts at: 0 80 903
Size: 80 823
AflIII A'CryG_T
Cuts at: 0 764 903
Size: 764 139
AleI CACnn'nnGTG
Cuts at: 0 529 649 903
Size: 529 120 254
Fragments arranged by size:
529 254 120
AlwNI CAG_nnn'CTG
Cuts at: 0 802 903
Size: 802 101
ApoI r'AATT_y
Cuts at: 0 898 903
Size: 898 5
BanI G'GyrC_C
Cuts at: 0 80 289 573 805 903
Size: 80 209 284 232 98
Fragments arranged by size:
284 232 209 98 80
BfrBI ATG'CAT
Cuts at: 0 500 903
Size: 500 403
BglII A'GATC_T
Cuts at: 0 659 903
Size: 659 244
BlpI GC'TnA_GC
Cuts at: 0 346 903
Size: 346 557
Bme1580I G_kGCm'C
Cuts at: 0 578 903
Size: 578 325
BmtI G_CTAG'C
Cuts at: 0 152 903
Size: 152 751
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 754 903
Size: 754 149
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 177 325 903
Size: 177 148 578
Fragments arranged by size:
578 177 148
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 215 903
Size: 215 688
BseYI C'CCAG_C
Cuts at: 0 549 903
Size: 549 354
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 470 815 876 903
Size: 448 22 345 61 27
Fragments arranged by size:
448 345 61 27 22
BsmI GAATG_Cn'
Cuts at: 0 184 705 903
Size: 184 521 198
Fragments arranged by size:
521 198 184
Bsp1286I G_dGCh'C
Cuts at: 0 578 903
Size: 578 325
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 68 903
Size: 68 835
BsrDI GCAATG_nn'
Cuts at: 0 815 903
Size: 815 88
BstXI CCAn_nnnn'nTGG
Cuts at: 0 651 903
Size: 651 252
BstYI r'GATC_y
Cuts at: 0 659 903
Size: 659 244
BtgI C'CryG_G
Cuts at: 0 293 644 903
Size: 293 351 259
Fragments arranged by size:
351 293 259
BtsI GCAGTG_nn'
Cuts at: 0 458 903
Size: 458 445
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 798 833 903
Size: 798 35 70
Fragments arranged by size:
798 70 35
DraI TTT'AAA
Cuts at: 0 477 903
Size: 477 426
EaeI y'GGCC_r
Cuts at: 0 224 492 903
Size: 224 268 411
Fragments arranged by size:
411 268 224
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 754 903
Size: 754 149
EcoRV GAT'ATC
Cuts at: 0 672 903
Size: 672 231
HincII GTy'rAC
Cuts at: 0 605 903
Size: 605 298
KpnI G_GTAC'C
Cuts at: 0 84 903
Size: 84 819
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 699 903
Size: 699 204
MscI TGG'CCA
Cuts at: 0 226 903
Size: 226 677
MslI CAynn'nnrTG
Cuts at: 0 529 649 903
Size: 529 120 254
Fragments arranged by size:
529 254 120
MspA1I CmG'CkG
Cuts at: 0 571 903
Size: 571 332
NdeI CA'TA_TG
Cuts at: 0 598 903
Size: 598 305
NheI G'CTAG_C
Cuts at: 0 148 903
Size: 148 755
NsiI A_TGCA'T
Cuts at: 0 502 903
Size: 502 401
NspI r_CATG'y
Cuts at: 0 277 768 903
Size: 277 491 135
Fragments arranged by size:
491 277 135
PasI CC'CwG_GG
Cuts at: 0 135 903
Size: 135 768
PciI A'CATG_T
Cuts at: 0 764 903
Size: 764 139
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 100 132 903
Size: 100 32 771
Fragments arranged by size:
771 100 32
PstI C_TGCA'G
Cuts at: 0 12 903
Size: 12 891
SfcI C'TryA_G
Cuts at: 0 8 903
Size: 8 895
SmlI C'TyrA_G
Cuts at: 0 156 304 903
Size: 156 148 599
Fragments arranged by size:
599 156 148
StyI C'CwwG_G
Cuts at: 0 694 903
Size: 694 209
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 541 903
Size: 541 362
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 633 665 903
Size: 633 32 238
Fragments arranged by size:
633 238 32
XmnI GAAnn'nnTTC
Cuts at: 0 703 903
Size: 703 200
Enzymes that do cut:
Acc65I AflIII AleI AlwNI ApoI BanI BfrBI BglII
BlpI Bme1580I BmtI BpmI BpuEI BseRI BseYI BsgI
BsmI Bsp1286I BspMI BsrDI BstXI BstYI BtgI BtsI
CspCI DraI EaeI Eco57MI EcoRV HincII KpnI MmeI
MscI MslI MspA1I NdeI NheI NsiI NspI PasI
PciI PsrI PstI SfcI SmlI StyI TaqII TstI
XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BmgBI BmrI BplI Bpu10I
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiHKAI
BsiWI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI
DraIII DrdI EagI EarI EciI EcoICRI EcoNI EcoO109I
EcoRI FalI FseI FspI FspAI HaeII Hin4I HindIII
HpaI KasI MfeI MluI NaeI NarI NcoI NgoMIV
NotI NruI PacI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI Tth111I XbaI XcmI
XhoI XmaI ZraI