(Linear) (Six-base) MAPSORT of: KIEE0464.seq  Check: 2904  from: 1  to: 666



KIEE0464.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AccI GT'mk_AC

Cuts at:      0     504     666 
   Size:        504     162
 
AclI AA'CG_TT

Cuts at:      0     531     666 
   Size:        531     135
 
AflII C'TTAA_G

Cuts at:      0     451     666 
   Size:        451     215
 
ApoI r'AATT_y

Cuts at:      0      48     391     666 
   Size:         48     343     275

  Fragments arranged by size: 

                 343     275      48
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     208     559     666 
   Size:        208     351     107

  Fragments arranged by size: 

                 351     208     107
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     483     666 
   Size:        483     183
 
BclI T'GATC_A

Cuts at:      0     584     666 
   Size:        584      82
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     396     666 
   Size:        396     270
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     266     666 
   Size:        266     400
 
BsaAI yAC'GTr

Cuts at:      0      78     666 
   Size:         78     588
 
BsiHKAI G_wGCw'C

Cuts at:      0     225     666 
   Size:        225     441
 
BsmI GAATG_Cn'

Cuts at:      0     147     666 
   Size:        147     519
 
Bsp1286I G_dGCh'C

Cuts at:      0     225     666 
   Size:        225     441
 
BsrDI GCAATG_nn'

Cuts at:      0     128     666 
   Size:        128     538
 
BsrFI r'CCGG_y

Cuts at:      0     148     666 
   Size:        148     518
 
BstBI TT'CG_AA

Cuts at:      0      52     666 
   Size:         52     614
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     367     666 
   Size:        367     299
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0       6     666 
   Size:          6     660
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     266     666 
   Size:        266     400
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     167     666 
   Size:        167     499
 
EcoRI G'AATT_C

Cuts at:      0     391     666 
   Size:        391     275
 
NaeI GCC'GGC

Cuts at:      0     150     666 
   Size:        150     516
 
NgoMIV G'CCGG_C

Cuts at:      0     148     666 
   Size:        148     518
 
PshAI GACnn'nnGTC

Cuts at:      0     632     666 
   Size:        632      34
 
SfcI C'TryA_G

Cuts at:      0     283     423     666 
   Size:        283     140     243

  Fragments arranged by size: 

                 283     243     140
 
SmlI C'TyrA_G

Cuts at:      0     451     666 
   Size:        451     215
 
SspI AAT'ATT

Cuts at:      0     231     666 
   Size:        231     435
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     536     666 
   Size:        536     130
 
XmnI GAAnn'nnTTC

Cuts at:      0     302     666 
   Size:        302     364
 

 Enzymes that do cut:

     AccI     AclI    AflII     ApoI     BbsI    BciVI     BclI     BmrI
     BpmI    BsaAI  BsiHKAI     BsmI Bsp1286I    BsrDI    BsrFI    BstBI
    BstXI     EciI  Eco57MI    EcoNI    EcoRI     NaeI   NgoMIV    PshAI
     SfcI     SmlI     SspI    TaqII     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AcuI     AfeI   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrGI   BssHII    BssSI   BstAPI   BstEII
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI  EcoICRI EcoO109I
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NarI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI