(Linear) (Six-base) MAPSORT of: KIEE0448.seq  Check: 1982  from: 1  to: 471



KIEE0448.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

Acc65I G'GTAC_C

Cuts at:      0      33      62     471 
   Size:         33      29     409

  Fragments arranged by size: 

                 409      33      29
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     390     471 
   Size:        390      81
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      67      99     471 
   Size:         67      32     372

  Fragments arranged by size: 

                 372      67      32
 
BamHI G'GATC_C

Cuts at:      0     330     471 
   Size:        330     141
 
BanI G'GyrC_C

Cuts at:      0      33      62      99     471 
   Size:         33      29      37     372

  Fragments arranged by size: 

                 372      37      33      29
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     124     471 
   Size:        124     347
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     471 
   Size:        169     302
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      67      97     471 
   Size:         67      30     374

  Fragments arranged by size: 

                 374      67      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      31      91     471 
   Size:         31      60     380

  Fragments arranged by size: 

                 380      60      31
 
BsiEI CG_ry'CG

Cuts at:      0     174     471 
   Size:        174     297
 
BsrBI CCG'CTC

Cuts at:      0      21     471 
   Size:         21     450
 
BsrFI r'CCGG_y

Cuts at:      0     146     471 
   Size:        146     325
 
BssHII G'CGCG_C

Cuts at:      0     114     471 
   Size:        114     357
 
BstYI r'GATC_y

Cuts at:      0     179     330     471 
   Size:        179     151     141

  Fragments arranged by size: 

                 179     151     141
 
BtgI C'CryG_G

Cuts at:      0      49     471 
   Size:         49     422
 
EaeI y'GGCC_r

Cuts at:      0     171     471 
   Size:        171     300
 
EagI C'GGCC_G

Cuts at:      0     171     471 
   Size:        171     300
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     124     390     471 
   Size:        124     266      81

  Fragments arranged by size: 

                 266     124      81
 
EcoO109I rG'GnC_Cy

Cuts at:      0      44     471 
   Size:         44     427
 
EcoRV GAT'ATC

Cuts at:      0     126     471 
   Size:        126     345
 
HaeII r_GCGC'y

Cuts at:      0     160     471 
   Size:        160     311
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     404     436     471 
   Size:        404      32      35

  Fragments arranged by size: 

                 404      35      32
 
HindIII A'AGCT_T

Cuts at:      0     361     471 
   Size:        361     110
 
KpnI G_GTAC'C

Cuts at:      0      37      66     471 
   Size:         37      29     405

  Fragments arranged by size: 

                 405      37      29
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     277     471 
   Size:        277     194
 
MspA1I CmG'CkG

Cuts at:      0     328     471 
   Size:        328     143
 
NaeI GCC'GGC

Cuts at:      0     148     471 
   Size:        148     323
 
NgoMIV G'CCGG_C

Cuts at:      0     146     471 
   Size:        146     325
 
NotI GC'GGCC_GC

Cuts at:      0     171     471 
   Size:        171     300
 
NspI r_CATG'y

Cuts at:      0     154     471 
   Size:        154     317
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      72     471 
   Size:         72     399
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      67      99     471 
   Size:         67      32     372

  Fragments arranged by size: 

                 372      67      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      44     471 
   Size:         44     427
 
SanDI GG'GwC_CC

Cuts at:      0      44     471 
   Size:         44     427
 
SmlI C'TyrA_G

Cuts at:      0     184     463     471 
   Size:        184     279       8

  Fragments arranged by size: 

                 279     184       8
 
StyI C'CwwG_G

Cuts at:      0      71     334     471 
   Size:         71     263     137

  Fragments arranged by size: 

                 263     137      71
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     327     471 
   Size:        327     144
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     115     147     471 
   Size:        115      32     324

  Fragments arranged by size: 

                 324     115      32
 

 Enzymes that do cut:

   Acc65I     AcuI     AloI    BamHI     BanI     BpmI    BpuEI    BsaXI
    BseRI    BsiEI    BsrBI    BsrFI   BssHII    BstYI     BtgI     EaeI
     EagI  Eco57MI EcoO109I    EcoRV    HaeII    Hin4I  HindIII     KpnI
     MmeI   MspA1I     NaeI   NgoMIV     NotI     NspI    PflMI     PpiI
    PpuMI    SanDI     SmlI     StyI    TaqII     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseYI     BsgI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrDI    BsrGI    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EarI     EciI  EcoICRI
    EcoNI    EcoRI     FalI     FseI     FspI    FspAI   HincII     HpaI
     KasI     MfeI     MluI     MscI     MslI     NarI     NcoI     NdeI
     NheI     NruI     NsiI     PacI     PasI     PciI     PfoI     PmeI
     PmlI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI