(Linear) (Six-base) MAPSORT of: KIEE0446.seq  Check: 5085  from: 1  to: 711



KIEE0446.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      29     353     545     711 
   Size:         29     324     192     166

  Fragments arranged by size: 

                 324     192     166      29
 
AlwNI CAG_nnn'CTG

Cuts at:      0     418     711 
   Size:        418     293
 
ApoI r'AATT_y

Cuts at:      0     148     711 
   Size:        148     563
 
BanII G_rGCy'C

Cuts at:      0     229     711 
   Size:        229     482
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      35     711 
   Size:         35     676
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     193     223     711 
   Size:        193      30     488

  Fragments arranged by size: 

                 488     193      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     589     711 
   Size:        589     122
 
BsiHKAI G_wGCw'C

Cuts at:      0     229     711 
   Size:        229     482
 
Bsp1286I G_dGCh'C

Cuts at:      0     229     711 
   Size:        229     482
 
BsrDI GCAATG_nn'

Cuts at:      0      20     711 
   Size:         20     691
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     141     711 
   Size:        141     570
 
BtsI GCAGTG_nn'

Cuts at:      0     609     711 
   Size:        609     102
 
DraI TTT'AAA

Cuts at:      0     443     711 
   Size:        443     268
 
EcoICRI GAG'CTC

Cuts at:      0     227     711 
   Size:        227     484
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      29     353     545     711 
   Size:         29     324     192     166

  Fragments arranged by size: 

                 324     192     166      29
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     214     246     538     570     711 
   Size:        214      32     292      32     141

  Fragments arranged by size: 

                 292     214     141      32      32
 
SacI G_AGCT'C

Cuts at:      0     229     711 
   Size:        229     482
 
StuI AGG'CCT

Cuts at:      0      84     711 
   Size:         84     627
 
StyI C'CwwG_G

Cuts at:      0      79     134     711 
   Size:         79      55     577

  Fragments arranged by size: 

                 577      79      55
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      86     711 
   Size:         86     625
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI    BanII     BbsI    BsaXI    BseRI  BsiHKAI
 Bsp1286I    BsrDI    BstXI     BtsI     DraI  EcoICRI  Eco57MI    Hin4I
     SacI     StuI     StyI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI    CspCI   DraIII     DrdI     EaeI
     EagI     EarI     EciI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI