Restriction map for
pF1KE0443
(Linear) (Six-base) MAPSORT of: KIEE0443.seq Check: 9990 from: 1 to: 966
KIEE0443.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 57 771 805 966
Size: 57 714 34 161
Fragments arranged by size:
714 161 57 34
ApaI G_GGCC'C
Cuts at: 0 440 966
Size: 440 526
ApaLI G'TGCA_C
Cuts at: 0 97 966
Size: 97 869
AvaI C'yCGr_G
Cuts at: 0 323 457 966
Size: 323 134 509
Fragments arranged by size:
509 323 134
BanI G'GyrC_C
Cuts at: 0 139 196 640 966
Size: 139 57 444 326
Fragments arranged by size:
444 326 139 57
BanII G_rGCy'C
Cuts at: 0 34 94 440 486 905 966
Size: 34 60 346 46 419 61
Fragments arranged by size:
419 346 61 60 46 34
BbeI G_GCGC'C
Cuts at: 0 143 200 966
Size: 143 57 766
Fragments arranged by size:
766 143 57
BglI GCCn_nnn'nGGC
Cuts at: 0 112 328 966
Size: 112 216 638
Fragments arranged by size:
638 216 112
BlpI GC'TnA_GC
Cuts at: 0 247 966
Size: 247 719
Bme1580I G_kGCm'C
Cuts at: 0 101 440 643 966
Size: 101 339 203 323
Fragments arranged by size:
339 323 203 101
BmgBI CAC'GTC
Cuts at: 0 373 966
Size: 373 593
BmrI ACTGGGnnnn_n'
Cuts at: 0 889 966
Size: 889 77
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 745 966
Size: 28 717 221
Fragments arranged by size:
717 221 28
BsaI GGTCTCn'nnnn_
Cuts at: 0 454 721 966
Size: 454 267 245
Fragments arranged by size:
454 267 245
BsaAI yAC'GTr
Cuts at: 0 690 966
Size: 690 276
BsaHI Gr'CG_yC
Cuts at: 0 140 197 966
Size: 140 57 769
Fragments arranged by size:
769 140 57
BsaWI w'CCGG_w
Cuts at: 0 791 905 966
Size: 791 114 61
Fragments arranged by size:
791 114 61
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 913 966
Size: 913 53
BseYI C'CCAG_C
Cuts at: 0 700 966
Size: 700 266
BsiEI CG_ry'CG
Cuts at: 0 760 966
Size: 760 206
BsiHKAI G_wGCw'C
Cuts at: 0 101 458 905 966
Size: 101 357 447 61
Fragments arranged by size:
447 357 101 61
BsmI GAATG_Cn'
Cuts at: 0 677 966
Size: 677 289
BsmBI CGTCTCn'nnnn_
Cuts at: 0 363 966
Size: 363 603
Bsp1286I G_dGCh'C
Cuts at: 0 34 94 101 440 458 486 643 905
Size: 34 60 7 339 18 28 157 262
Cuts at: 905 966
Size: 61
Fragments arranged by size:
339 262 157 61 60 34 28 18
7
BspEI T'CCGG_A
Cuts at: 0 791 905 966
Size: 791 114 61
Fragments arranged by size:
791 114 61
BsrFI r'CCGG_y
Cuts at: 0 730 966
Size: 730 236
BssSI C'ACGA_G
Cuts at: 0 223 966
Size: 223 743
BstXI CCAn_nnnn'nTGG
Cuts at: 0 156 636 966
Size: 156 480 330
Fragments arranged by size:
480 330 156
BstYI r'GATC_y
Cuts at: 0 41 518 966
Size: 41 477 448
Fragments arranged by size:
477 448 41
Bsu36I CC'TnA_GG
Cuts at: 0 896 966
Size: 896 70
BtgI C'CryG_G
Cuts at: 0 179 560 672 966
Size: 179 381 112 294
Fragments arranged by size:
381 294 179 112
BtsI GCAGTG_nn'
Cuts at: 0 427 966
Size: 427 539
EaeI y'GGCC_r
Cuts at: 0 146 653 966
Size: 146 507 313
Fragments arranged by size:
507 313 146
EarI CTCTTCn'nnn_
Cuts at: 0 347 395 711 746 936 966
Size: 347 48 316 35 190 30
Fragments arranged by size:
347 316 190 48 35 30
EcoICRI GAG'CTC
Cuts at: 0 903 966
Size: 903 63
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 745 966
Size: 28 717 221
Fragments arranged by size:
717 221 28
EcoO109I rG'GnC_Cy
Cuts at: 0 436 437 966
Size: 436 1 529
Fragments arranged by size:
529 436 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 267 966
Size: 267 699
HaeII r_GCGC'y
Cuts at: 0 143 200 347 966
Size: 143 57 147 619
Fragments arranged by size:
619 147 143 57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 197 229 966
Size: 197 32 737
Fragments arranged by size:
737 197 32
HincII GTy'rAC
Cuts at: 0 282 966
Size: 282 684
KasI G'GCGC_C
Cuts at: 0 139 196 966
Size: 139 57 770
Fragments arranged by size:
770 139 57
MscI TGG'CCA
Cuts at: 0 655 966
Size: 655 311
MslI CAynn'nnrTG
Cuts at: 0 130 966
Size: 130 836
MspA1I CmG'CkG
Cuts at: 0 54 277 583 651 966
Size: 54 223 306 68 315
Fragments arranged by size:
315 306 223 68 54
NarI GG'CG_CC
Cuts at: 0 140 197 966
Size: 140 57 769
Fragments arranged by size:
769 140 57
NcoI C'CATG_G
Cuts at: 0 560 672 966
Size: 560 112 294
Fragments arranged by size:
560 294 112
PasI CC'CwG_GG
Cuts at: 0 441 966
Size: 441 525
PfoI T'CCnGG_A
Cuts at: 0 362 966
Size: 362 604
PshAI GACnn'nnGTC
Cuts at: 0 719 966
Size: 719 247
PspOMI G'GGCC_C
Cuts at: 0 436 966
Size: 436 530
PstI C_TGCA'G
Cuts at: 0 41 191 966
Size: 41 150 775
Fragments arranged by size:
775 150 41
PvuII CAG'CTG
Cuts at: 0 54 583 651 966
Size: 54 529 68 315
Fragments arranged by size:
529 315 68 54
RsrII CG'GwC_CG
Cuts at: 0 360 966
Size: 360 606
SacI G_AGCT'C
Cuts at: 0 905 966
Size: 905 61
SapI GCTCTTCn'nnn_
Cuts at: 0 395 711 746 966
Size: 395 316 35 220
Fragments arranged by size:
395 316 220 35
SfcI C'TryA_G
Cuts at: 0 37 187 966
Size: 37 150 779
Fragments arranged by size:
779 150 37
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 112 966
Size: 112 854
SfoI GGC'GCC
Cuts at: 0 141 198 966
Size: 141 57 768
Fragments arranged by size:
768 141 57
SmaI CCC'GGG
Cuts at: 0 325 966
Size: 325 641
SmlI C'TyrA_G
Cuts at: 0 457 966
Size: 457 509
StuI AGG'CCT
Cuts at: 0 106 966
Size: 106 860
StyI C'CwwG_G
Cuts at: 0 560 672 966
Size: 560 112 294
Fragments arranged by size:
560 294 112
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 790 966
Size: 790 176
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 156 966
Size: 156 810
XhoI C'TCGA_G
Cuts at: 0 457 966
Size: 457 509
XmaI C'CCGG_G
Cuts at: 0 323 966
Size: 323 643
Enzymes that do cut:
AlwNI ApaI ApaLI AvaI BanI BanII BbeI BglI
BlpI Bme1580I BmgBI BmrI BpmI BsaI BsaAI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BsrFI BssSI BstXI BstYI Bsu36I BtgI BtsI
EaeI EarI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I
HincII KasI MscI MslI MspA1I NarI NcoI PasI
PfoI PshAI PspOMI PstI PvuII RsrII SacI SapI
SfcI SfiI SfoI SmaI SmlI StuI StyI TaqII
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BmtI BplI Bpu10I BpuEI
BsaBI BsaXI BsgI BsiWI BspHI BspMI BsrBI BsrDI
BsrGI BssHII BstAPI BstBI BstEII BstZ17I BtgZI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoNI EcoRI
EcoRV FseI FspI FspAI HindIII HpaI KpnI MfeI
MluI MmeI NaeI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PflMI PmeI PmlI PpiI
PpuMI PsiI PspXI PsrI PvuI SacII SalI SanDI
SbfI ScaI SexAI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI TatI TstI Tth111I XbaI XmnI
ZraI