Restriction map for
pF1KE0436
(Linear) (Six-base) MAPSORT of: KIEE0436.seq Check: 7897 from: 1 to: 1092
KIEE0436.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 0 170 1092
Size: 170 922
AclI AA'CG_TT
Cuts at: 0 849 1092
Size: 849 243
AfeI AGC'GCT
Cuts at: 0 146 1092
Size: 146 946
AflII C'TTAA_G
Cuts at: 0 981 1092
Size: 981 111
AflIII A'CryG_T
Cuts at: 0 23 83 339 603 1092
Size: 23 60 256 264 489
Fragments arranged by size:
489 264 256 60 23
AleI CACnn'nnGTG
Cuts at: 0 82 1092
Size: 82 1010
ApoI r'AATT_y
Cuts at: 0 436 745 770 880 1092
Size: 436 309 25 110 212
Fragments arranged by size:
436 309 212 110 25
BanI G'GyrC_C
Cuts at: 0 72 1092
Size: 72 1020
BbeI G_GCGC'C
Cuts at: 0 76 1092
Size: 76 1016
BbsI GAAGACnn'nnnn_
Cuts at: 0 964 1092
Size: 964 128
BlpI GC'TnA_GC
Cuts at: 0 401 1092
Size: 401 691
Bme1580I G_kGCm'C
Cuts at: 0 70 1092
Size: 70 1022
BsaAI yAC'GTr
Cuts at: 0 26 84 351 477 1092
Size: 26 58 267 126 615
Fragments arranged by size:
615 267 126 58 26
BsaHI Gr'CG_yC
Cuts at: 0 73 1092
Size: 73 1019
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 13 43 1092
Size: 13 30 1049
Fragments arranged by size:
1049 30 13
BseYI C'CCAG_C
Cuts at: 0 49 1092
Size: 49 1043
BsiEI CG_ry'CG
Cuts at: 0 156 1092
Size: 156 936
BsiHKAI G_wGCw'C
Cuts at: 0 20 62 188 1092
Size: 20 42 126 904
Fragments arranged by size:
904 126 42 20
BsmI GAATG_Cn'
Cuts at: 0 333 1092
Size: 333 759
Bsp1286I G_dGCh'C
Cuts at: 0 20 62 70 188 1092
Size: 20 42 8 118 904
Fragments arranged by size:
904 118 42 20 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 50 1092
Size: 50 1042
BsrFI r'CCGG_y
Cuts at: 0 155 1092
Size: 155 937
BstAPI GCAn_nnn'nTGC
Cuts at: 0 574 1092
Size: 574 518
BstBI TT'CG_AA
Cuts at: 0 854 1092
Size: 854 238
BstYI r'GATC_y
Cuts at: 0 897 1092
Size: 897 195
BtsI GCAGTG_nn'
Cuts at: 0 435 1092
Size: 435 657
EaeI y'GGCC_r
Cuts at: 0 314 1092
Size: 314 778
EarI CTCTTCn'nnn_
Cuts at: 0 193 717 1092
Size: 193 524 375
Fragments arranged by size:
524 375 193
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 105 1092
Size: 105 987
HaeII r_GCGC'y
Cuts at: 0 76 148 1092
Size: 76 72 944
Fragments arranged by size:
944 76 72
HincII GTy'rAC
Cuts at: 0 291 1092
Size: 291 801
KasI G'GCGC_C
Cuts at: 0 72 1092
Size: 72 1020
MscI TGG'CCA
Cuts at: 0 316 1092
Size: 316 776
MslI CAynn'nnrTG
Cuts at: 0 82 1006 1092
Size: 82 924 86
Fragments arranged by size:
924 86 82
NarI GG'CG_CC
Cuts at: 0 73 1092
Size: 73 1019
NdeI CA'TA_TG
Cuts at: 0 827 1092
Size: 827 265
NspI r_CATG'y
Cuts at: 0 343 607 1005 1092
Size: 343 264 398 87
Fragments arranged by size:
398 343 264 87
PciI A'CATG_T
Cuts at: 0 339 603 1092
Size: 339 264 489
Fragments arranged by size:
489 339 264
PflMI CCAn_nnn'nTGG
Cuts at: 0 147 180 385 1092
Size: 147 33 205 707
Fragments arranged by size:
707 205 147 33
PstI C_TGCA'G
Cuts at: 0 999 1092
Size: 999 93
SapI GCTCTTCn'nnn_
Cuts at: 0 193 1092
Size: 193 899
SfcI C'TryA_G
Cuts at: 0 995 1092
Size: 995 97
SfoI GGC'GCC
Cuts at: 0 74 1092
Size: 74 1018
SmlI C'TyrA_G
Cuts at: 0 981 1092
Size: 981 111
SphI G_CATG'C
Cuts at: 0 1005 1092
Size: 1005 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 377 1027 1092
Size: 377 650 65
Fragments arranged by size:
650 377 65
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 13 45 1092
Size: 13 32 1047
Fragments arranged by size:
1047 32 13
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 84 1092
Size: 84 1008
Enzymes that do cut:
AccI AclI AfeI AflII AflIII AleI ApoI BanI
BbeI BbsI BlpI Bme1580I BsaAI BsaHI BsaXI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrFI BstAPI BstBI
BstYI BtsI EaeI EarI EciI HaeII HincII KasI
MscI MslI NarI NdeI NspI PciI PflMI PstI
SapI SfcI SfoI SmlI SphI TaqII TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AcuI AgeI AhdI AlfI AloI
AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanII BbvCI BcgI BciVI BclI BfrBI
BglI BglII BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaBI BsaWI BseRI BsgI BsiWI BsmBI
BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstEII
BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI
DraIII DrdI EagI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HindIII HpaI
KpnI MfeI MluI MmeI MspA1I NaeI NcoI NgoMIV
NheI NotI NruI NsiI PacI PasI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI StuI StyI SwaI TatI Tth111I XbaI
XhoI XmaI XmnI ZraI