Restriction map for
pF1KE0429
(Linear) (Six-base) MAPSORT of: KIEE0429.seq Check: 6443 from: 1 to: 1284
KIEE0429.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1215 1284
Size: 1215 69
Acc65I G'GTAC_C
Cuts at: 0 665 1284
Size: 665 619
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 479 1284
Size: 479 805
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1234 1266 1284
Size: 1234 32 18
Fragments arranged by size:
1234 32 18
ApaI G_GGCC'C
Cuts at: 0 19 102 263 1284
Size: 19 83 161 1021
Fragments arranged by size:
1021 161 83 19
ApaLI G'TGCA_C
Cuts at: 0 728 1284
Size: 728 556
AvaI C'yCGr_G
Cuts at: 0 184 256 262 1284
Size: 184 72 6 1022
Fragments arranged by size:
1022 184 72 6
BanI G'GyrC_C
Cuts at: 0 665 1227 1284
Size: 665 562 57
Fragments arranged by size:
665 562 57
BanII G_rGCy'C
Cuts at: 0 19 102 263 343 1284
Size: 19 83 161 80 941
Fragments arranged by size:
941 161 83 80 19
BbsI GAAGACnn'nnnn_
Cuts at: 0 689 1284
Size: 689 595
BbvCI CC'TCA_GC
Cuts at: 0 1208 1284
Size: 1208 76
BclI T'GATC_A
Cuts at: 0 168 1284
Size: 168 1116
BglI GCCn_nnn'nGGC
Cuts at: 0 621 1284
Size: 621 663
Bme1580I G_kGCm'C
Cuts at: 0 19 102 257 263 732 1232 1279 1284
Size: 19 83 155 6 469 500 47 5
Fragments arranged by size:
500 469 155 83 47 19 6 5
BmrI ACTGGGnnnn_n'
Cuts at: 0 501 584 836 1284
Size: 501 83 252 448
Fragments arranged by size:
501 448 252 83
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 351 1195 1284
Size: 351 844 89
Fragments arranged by size:
844 351 89
Bpu10I CC'TnA_GC
Cuts at: 0 128 903 1208 1284
Size: 128 775 305 76
Fragments arranged by size:
775 305 128 76
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 1284
Size: 88 1196
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1095 1125 1284
Size: 1095 30 159
Fragments arranged by size:
1095 159 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 426 555 969 972 1284
Size: 426 129 414 3 312
Fragments arranged by size:
426 414 312 129 3
BseYI C'CCAG_C
Cuts at: 0 660 1059 1284
Size: 660 399 225
Fragments arranged by size:
660 399 225
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 330 1284
Size: 330 954
BsiHKAI G_wGCw'C
Cuts at: 0 343 732 1284
Size: 343 389 552
Fragments arranged by size:
552 389 343
Bsp1286I G_dGCh'C
Cuts at: 0 19 102 257 263 343 732 1232 1279
Size: 19 83 155 6 80 389 500 47
Cuts at: 1279 1284
Size: 5
Fragments arranged by size:
500 389 155 83 80 47 19 6
5
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 726 1215 1284
Size: 726 489 69
Fragments arranged by size:
726 489 69
BsrBI CCG'CTC
Cuts at: 0 113 1142 1284
Size: 113 1029 142
Fragments arranged by size:
1029 142 113
BssSI C'ACGA_G
Cuts at: 0 1272 1284
Size: 1272 12
BstAPI GCAn_nnn'nTGC
Cuts at: 0 208 913 1227 1284
Size: 208 705 314 57
Fragments arranged by size:
705 314 208 57
BstEII G'GTnAC_C
Cuts at: 0 189 1032 1284
Size: 189 843 252
Fragments arranged by size:
843 252 189
BstYI r'GATC_y
Cuts at: 0 987 1249 1284
Size: 987 262 35
Fragments arranged by size:
987 262 35
Bsu36I CC'TnA_GG
Cuts at: 0 482 553 1284
Size: 482 71 731
Fragments arranged by size:
731 482 71
BtgI C'CryG_G
Cuts at: 0 383 1284
Size: 383 901
BtsI GCAGTG_nn'
Cuts at: 0 432 730 1284
Size: 432 298 554
Fragments arranged by size:
554 432 298
DraIII CAC_nnn'GTG
Cuts at: 0 509 1284
Size: 509 775
DrdI GACnn_nn'nnGTC
Cuts at: 0 148 870 1284
Size: 148 722 414
Fragments arranged by size:
722 414 148
EarI CTCTTCn'nnn_
Cuts at: 0 498 1011 1284
Size: 498 513 273
Fragments arranged by size:
513 498 273
EcoICRI GAG'CTC
Cuts at: 0 341 1284
Size: 341 943
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 351 479 1195 1284
Size: 351 128 716 89
Fragments arranged by size:
716 351 128 89
EcoO109I rG'GnC_Cy
Cuts at: 0 15 98 99 141 180 921 967 1188
Size: 15 83 1 42 39 741 46 221
Cuts at: 1188 1284
Size: 96
Fragments arranged by size:
741 221 96 83 46 42 39 15
1
KpnI G_GTAC'C
Cuts at: 0 669 1284
Size: 669 615
MspA1I CmG'CkG
Cuts at: 0 441 1063 1120 1218 1284
Size: 441 622 57 98 66
Fragments arranged by size:
622 441 98 66 57
PfoI T'CCnGG_A
Cuts at: 0 331 1261 1284
Size: 331 930 23
Fragments arranged by size:
930 331 23
PpuMI rG'GwC_Cy
Cuts at: 0 141 180 921 1284
Size: 141 39 741 363
Fragments arranged by size:
741 363 141 39
PshAI GACnn'nnGTC
Cuts at: 0 74 1284
Size: 74 1210
PspOMI G'GGCC_C
Cuts at: 0 15 98 259 1284
Size: 15 83 161 1025
Fragments arranged by size:
1025 161 83 15
PstI C_TGCA'G
Cuts at: 0 275 737 766 918 1119 1223 1284
Size: 275 462 29 152 201 104 61
Fragments arranged by size:
462 275 201 152 104 61 29
PvuII CAG'CTG
Cuts at: 0 441 1063 1120 1218 1284
Size: 441 622 57 98 66
Fragments arranged by size:
622 441 98 66 57
SacI G_AGCT'C
Cuts at: 0 343 1284
Size: 343 941
SanDI GG'GwC_CC
Cuts at: 0 180 921 1284
Size: 180 741 363
Fragments arranged by size:
741 363 180
SfcI C'TryA_G
Cuts at: 0 271 375 733 762 914 929 1115 1219
Size: 271 104 358 29 152 15 186 104
Cuts at: 1219 1284
Size: 65
Fragments arranged by size:
358 271 186 152 104 104 65 29
15
SmaI CCC'GGG
Cuts at: 0 258 264 1284
Size: 258 6 1020
Fragments arranged by size:
1020 258 6
SmlI C'TyrA_G
Cuts at: 0 103 1284
Size: 103 1181
SrfI GCCC'GGGC
Cuts at: 0 258 264 1284
Size: 258 6 1020
Fragments arranged by size:
1020 258 6
StuI AGG'CCT
Cuts at: 0 239 480 580 1284
Size: 239 241 100 704
Fragments arranged by size:
704 241 239 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 520 855 1284
Size: 520 335 429
Fragments arranged by size:
520 429 335
TatI w'GTAC_w
Cuts at: 0 592 1284
Size: 592 692
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 27 775 807 1095 1127 1284
Size: 27 748 32 288 32 157
Fragments arranged by size:
748 288 157 32 32 27
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 160 1284
Size: 160 1124
XmaI C'CCGG_G
Cuts at: 0 256 262 1284
Size: 256 6 1022
Fragments arranged by size:
1022 256 6
Enzymes that do cut:
AarI Acc65I AcuI AloI ApaI ApaLI AvaI BanI
BanII BbsI BbvCI BclI BglI Bme1580I BmrI BpmI
Bpu10I BpuEI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspMI BsrBI BssSI BstAPI BstEII BstYI Bsu36I BtgI
BtsI DraIII DrdI EarI EcoICRI Eco57MI EcoO109I KpnI
MspA1I PfoI PpuMI PshAI PspOMI PstI PvuII SacI
SanDI SfcI SmaI SmlI SrfI StuI TaqII TatI
TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AlwNI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BcgI BciVI BfrBI BglII BlpI
BmgBI BmtI BplI BsaI BsaAI BsaBI BsaHI BsaWI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrFI
BsrGI BssHII BstBI BstXI BstZ17I BtgZI ClaI CspCI
DraI EaeI EagI EciI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI MfeI MluI MmeI MscI MslI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PmeI PmlI PpiI PsiI
PspXI PsrI PvuI RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SspI StyI SwaI Tth111I XbaI XhoI XmnI
ZraI