Restriction map for
pF1KE0423
(Linear) (Six-base) MAPSORT of: KIEE0423.seq Check: 2623 from: 1 to: 705
KIEE0423.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 267 705
Size: 267 438
AvaI C'yCGr_G
Cuts at: 0 67 284 705
Size: 67 217 421
Fragments arranged by size:
421 217 67
BanI G'GyrC_C
Cuts at: 0 245 479 520 705
Size: 245 234 41 185
Fragments arranged by size:
245 234 185 41
BanII G_rGCy'C
Cuts at: 0 404 568 705
Size: 404 164 137
Fragments arranged by size:
404 164 137
BbeI G_GCGC'C
Cuts at: 0 249 705
Size: 249 456
BbvCI CC'TCA_GC
Cuts at: 0 134 472 634 705
Size: 134 338 162 71
Fragments arranged by size:
338 162 134 71
Bme1580I G_kGCm'C
Cuts at: 0 203 705
Size: 203 502
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 705
Size: 505 200
Bpu10I CC'TnA_GC
Cuts at: 0 134 472 567 634 705
Size: 134 338 95 67 71
Fragments arranged by size:
338 134 95 71 67
BsaI GGTCTCn'nnnn_
Cuts at: 0 643 705
Size: 643 62
BsaAI yAC'GTr
Cuts at: 0 510 705
Size: 510 195
BsaHI Gr'CG_yC
Cuts at: 0 246 428 705
Size: 246 182 277
Fragments arranged by size:
277 246 182
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 685 705
Size: 685 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 46 601 705
Size: 46 555 104
Fragments arranged by size:
555 104 46
BseYI C'CCAG_C
Cuts at: 0 116 516 705
Size: 116 400 189
Fragments arranged by size:
400 189 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 705
Size: 340 365
BsiHKAI G_wGCw'C
Cuts at: 0 568 664 705
Size: 568 96 41
Fragments arranged by size:
568 96 41
Bsp1286I G_dGCh'C
Cuts at: 0 203 404 568 664 705
Size: 203 201 164 96 41
Fragments arranged by size:
203 201 164 96 41
BsrDI GCAATG_nn'
Cuts at: 0 18 128 705
Size: 18 110 577
Fragments arranged by size:
577 110 18
BsrFI r'CCGG_y
Cuts at: 0 242 705
Size: 242 463
BstAPI GCAn_nnn'nTGC
Cuts at: 0 513 705
Size: 513 192
BtgI C'CryG_G
Cuts at: 0 99 204 705
Size: 99 105 501
Fragments arranged by size:
501 105 99
BtsI GCAGTG_nn'
Cuts at: 0 45 705
Size: 45 660
DraIII CAC_nnn'GTG
Cuts at: 0 513 705
Size: 513 192
EaeI y'GGCC_r
Cuts at: 0 170 461 705
Size: 170 291 244
Fragments arranged by size:
291 244 170
EarI CTCTTCn'nnn_
Cuts at: 0 176 331 705
Size: 176 155 374
Fragments arranged by size:
374 176 155
EcoICRI GAG'CTC
Cuts at: 0 566 705
Size: 566 139
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 705
Size: 505 200
EcoNI CCTnn'n_nnAGG
Cuts at: 0 637 705
Size: 637 68
EcoO109I rG'GnC_Cy
Cuts at: 0 27 186 447 594 630 705
Size: 27 159 261 147 36 75
Fragments arranged by size:
261 159 147 75 36 27
HaeII r_GCGC'y
Cuts at: 0 249 705
Size: 249 456
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 265 297 685 705
Size: 265 32 388 20
Fragments arranged by size:
388 265 32 20
KasI G'GCGC_C
Cuts at: 0 245 705
Size: 245 460
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 392 705
Size: 392 313
MscI TGG'CCA
Cuts at: 0 172 705
Size: 172 533
MslI CAynn'nnrTG
Cuts at: 0 457 705
Size: 457 248
NarI GG'CG_CC
Cuts at: 0 246 705
Size: 246 459
NcoI C'CATG_G
Cuts at: 0 99 204 705
Size: 99 105 501
Fragments arranged by size:
501 105 99
NruI TCG'CGA
Cuts at: 0 584 705
Size: 584 121
NspI r_CATG'y
Cuts at: 0 42 331 705
Size: 42 289 374
Fragments arranged by size:
374 289 42
PmlI CAC'GTG
Cuts at: 0 510 705
Size: 510 195
PpuMI rG'GwC_Cy
Cuts at: 0 27 186 447 630 705
Size: 27 159 261 183 75
Fragments arranged by size:
261 183 159 75 27
SacI G_AGCT'C
Cuts at: 0 568 705
Size: 568 137
SfoI GGC'GCC
Cuts at: 0 247 705
Size: 247 458
SgrAI Cr'CCGG_yG
Cuts at: 0 242 705
Size: 242 463
SmlI C'TyrA_G
Cuts at: 0 284 705
Size: 284 421
StuI AGG'CCT
Cuts at: 0 280 577 688 705
Size: 280 297 111 17
Fragments arranged by size:
297 280 111 17
StyI C'CwwG_G
Cuts at: 0 99 204 705
Size: 99 105 501
Fragments arranged by size:
501 105 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 241 508 705
Size: 241 267 197
Fragments arranged by size:
267 241 197
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 349 381 705
Size: 349 32 324
Fragments arranged by size:
349 324 32
XhoI C'TCGA_G
Cuts at: 0 284 705
Size: 284 421
Enzymes that do cut:
AlwNI AvaI BanI BanII BbeI BbvCI Bme1580I BpmI
Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BsrDI BsrFI BstAPI BtgI BtsI DraIII
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I
KasI MmeI MscI MslI NarI NcoI NruI NspI
PmlI PpuMI SacI SfoI SgrAI SmlI StuI StyI
TaqII TstI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI
BmrI BmtI BplI BpuEI BsaBI BsaWI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrGI BssHII
BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DrdI EagI EciI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI MspA1I NaeI NdeI NgoMIV NheI NotI
NsiI PacI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SgfI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TatI Tth111I XbaI XcmI XmaI XmnI
ZraI