Restriction map for
pF1KE0420
(Linear) (Six-base) MAPSORT of: KIEE0420.seq Check: 1410 from: 1 to: 618
KIEE0420.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 474 618
Size: 474 144
Acc65I G'GTAC_C
Cuts at: 0 77 618
Size: 77 541
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 167 194 618
Size: 167 27 424
Fragments arranged by size:
424 167 27
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 174 207 618
Size: 174 33 411
Fragments arranged by size:
411 174 33
BanI G'GyrC_C
Cuts at: 0 77 235 618
Size: 77 158 383
Fragments arranged by size:
383 158 77
BanII G_rGCy'C
Cuts at: 0 335 614 618
Size: 335 279 4
Fragments arranged by size:
335 279 4
BbeI G_GCGC'C
Cuts at: 0 239 618
Size: 239 379
BbsI GAAGACnn'nnnn_
Cuts at: 0 299 618
Size: 299 319
BglII A'GATC_T
Cuts at: 0 285 618
Size: 285 333
Bme1580I G_kGCm'C
Cuts at: 0 74 618
Size: 74 544
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 580 618
Size: 160 420 38
Fragments arranged by size:
420 160 38
BsaAI yAC'GTr
Cuts at: 0 576 618
Size: 576 42
BsaHI Gr'CG_yC
Cuts at: 0 236 618
Size: 236 382
BsaWI w'CCGG_w
Cuts at: 0 455 618
Size: 455 163
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 133 163 618
Size: 133 30 455
Fragments arranged by size:
455 133 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 115 618
Size: 115 503
BseYI C'CCAG_C
Cuts at: 0 27 227 334 618
Size: 27 200 107 284
Fragments arranged by size:
284 200 107 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 183 618
Size: 183 435
Bsp1286I G_dGCh'C
Cuts at: 0 74 335 614 618
Size: 74 261 279 4
Fragments arranged by size:
279 261 74 4
BspEI T'CCGG_A
Cuts at: 0 455 618
Size: 455 163
BspHI T'CATG_A
Cuts at: 0 320 618
Size: 320 298
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 474 618
Size: 474 144
BssSI C'ACGA_G
Cuts at: 0 82 618
Size: 82 536
BstEII G'GTnAC_C
Cuts at: 0 513 618
Size: 513 105
BstXI CCAn_nnnn'nTGG
Cuts at: 0 576 618
Size: 576 42
BstYI r'GATC_y
Cuts at: 0 285 618
Size: 285 333
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 391 618
Size: 391 227
BtsI GCAGTG_nn'
Cuts at: 0 166 504 618
Size: 166 338 114
Fragments arranged by size:
338 166 114
EarI CTCTTCn'nnn_
Cuts at: 0 239 280 575 618
Size: 239 41 295 43
Fragments arranged by size:
295 239 43 41
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 580 618
Size: 160 420 38
Fragments arranged by size:
420 160 38
EcoNI CCTnn'n_nnAGG
Cuts at: 0 142 618
Size: 142 476
EcoO109I rG'GnC_Cy
Cuts at: 0 351 618
Size: 351 267
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 210 618
Size: 210 408
HaeII r_GCGC'y
Cuts at: 0 239 618
Size: 239 379
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 26 437 469 618
Size: 26 411 32 149
Fragments arranged by size:
411 149 32 26
KasI G'GCGC_C
Cuts at: 0 235 618
Size: 235 383
KpnI G_GTAC'C
Cuts at: 0 81 618
Size: 81 537
MslI CAynn'nnrTG
Cuts at: 0 457 618
Size: 457 161
MspA1I CmG'CkG
Cuts at: 0 132 167 338 618
Size: 132 35 171 280
Fragments arranged by size:
280 171 132 35
NarI GG'CG_CC
Cuts at: 0 236 618
Size: 236 382
NspI r_CATG'y
Cuts at: 0 70 618
Size: 70 548
PmlI CAC'GTG
Cuts at: 0 576 618
Size: 576 42
PstI C_TGCA'G
Cuts at: 0 110 343 618
Size: 110 233 275
Fragments arranged by size:
275 233 110
PvuII CAG'CTG
Cuts at: 0 167 338 618
Size: 167 171 280
Fragments arranged by size:
280 171 167
SfcI C'TryA_G
Cuts at: 0 106 339 618
Size: 106 233 279
Fragments arranged by size:
279 233 106
SfoI GGC'GCC
Cuts at: 0 237 618
Size: 237 381
StyI C'CwwG_G
Cuts at: 0 302 354 618
Size: 302 52 264
Fragments arranged by size:
302 264 52
XbaI T'CTAG_A
Cuts at: 0 288 618
Size: 288 330
XmnI GAAnn'nnTTC
Cuts at: 0 274 618
Size: 274 344
Enzymes that do cut:
AarI Acc65I AlfI BaeI BanI BanII BbeI BbsI
BglII Bme1580I BpmI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI Bsp1286I BspEI BspHI BspMI BssSI BstEII
BstXI BstYI BtgZI BtsI EarI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I KasI KpnI MslI MspA1I NarI
NspI PmlI PstI PvuII SfcI SfoI StyI XbaI
XmnI
Enzymes that do not cut:
AatII AccI AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BamHI BbvCI BcgI BciVI
BclI BfrBI BglI BlpI BmgBI BmrI BmtI BplI
Bpu10I BpuEI BsaI BsaBI BsiEI BsiHKAI BsiWI BsmI
BsmBI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI
BstZ17I Bsu36I BtgI ClaI CspCI DraI DraIII DrdI
EaeI EagI EciI EcoICRI EcoRI EcoRV FseI FspI
FspAI HincII HindIII HpaI MfeI MluI MmeI MscI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TatI TstI Tth111I XcmI
XhoI XmaI ZraI