(Linear) (Six-base) MAPSORT of: KIEE0406.seq  Check: 4350  from: 1  to: 1023



KIEE0406.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     391    1023 
   Size:        391     632
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     340     360    1023 
   Size:        340      20     663

  Fragments arranged by size: 

                 663     340      20
 
AflIII A'CryG_T

Cuts at:      0     341    1023 
   Size:        341     682
 
AlwNI CAG_nnn'CTG

Cuts at:      0     476    1023 
   Size:        476     547
 
ApaI G_GGCC'C

Cuts at:      0     386    1023 
   Size:        386     637
 
ApoI r'AATT_y

Cuts at:      0     271     480     577    1023 
   Size:        271     209      97     446

  Fragments arranged by size: 

                 446     271     209      97
 
BanI G'GyrC_C

Cuts at:      0     391    1023 
   Size:        391     632
 
BanII G_rGCy'C

Cuts at:      0     386    1023 
   Size:        386     637
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     366     805    1023 
   Size:        366     439     218

  Fragments arranged by size: 

                 439     366     218
 
BbvCI CC'TCA_GC

Cuts at:      0     396    1023 
   Size:        396     627
 
Bme1580I G_kGCm'C

Cuts at:      0     386     449    1023 
   Size:        386      63     574

  Fragments arranged by size: 

                 574     386      63
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     123    1023 
   Size:        123     900
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     984    1023 
   Size:        984      39
 
Bpu10I CC'TnA_GC

Cuts at:      0     396    1023 
   Size:        396     627
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      21    1023 
   Size:         21    1002
 
BsaBI GATnn'nnATC

Cuts at:      0     527    1023 
   Size:        527     496
 
BsaWI w'CCGG_w

Cuts at:      0     585    1023 
   Size:        585     438
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     107     137    1023 
   Size:        107      30     886

  Fragments arranged by size: 

                 886     107      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      94    1023 
   Size:         94     929
 
BseYI C'CCAG_C

Cuts at:      0     250     413    1023 
   Size:        250     163     610

  Fragments arranged by size: 

                 610     250     163
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     260    1023 
   Size:        260     763
 
Bsp1286I G_dGCh'C

Cuts at:      0     386     449    1023 
   Size:        386      63     574

  Fragments arranged by size: 

                 574     386      63
 
BspEI T'CCGG_A

Cuts at:      0     585    1023 
   Size:        585     438
 
BspHI T'CATG_A

Cuts at:      0     794    1023 
   Size:        794     229
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     391    1023 
   Size:        391     632
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     136     873    1023 
   Size:        136     737     150

  Fragments arranged by size: 

                 737     150     136
 
Bsu36I CC'TnA_GG

Cuts at:      0     977    1023 
   Size:        977      46
 
BtsI GCAGTG_nn'

Cuts at:      0     447     687     713    1023 
   Size:        447     240      26     310

  Fragments arranged by size: 

                 447     310     240      26
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     662     697    1023 
   Size:        662      35     326

  Fragments arranged by size: 

                 662     326      35
 
EaeI y'GGCC_r

Cuts at:      0     832    1023 
   Size:        832     191
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     340     360    1023 
   Size:        340      20     663

  Fragments arranged by size: 

                 663     340      20
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      37    1023 
   Size:         37     986
 
EcoO109I rG'GnC_Cy

Cuts at:      0     382     880    1023 
   Size:        382     498     143

  Fragments arranged by size: 

                 498     382     143
 
EcoRI G'AATT_C

Cuts at:      0     480    1023 
   Size:        480     543
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     792     824     842     874    1023 
   Size:        792      32      18      32     149

  Fragments arranged by size: 

                 792     149      32      32      18
 
MscI TGG'CCA

Cuts at:      0     834    1023 
   Size:        834     189
 
MspA1I CmG'CkG

Cuts at:      0     325    1023 
   Size:        325     698
 
NspI r_CATG'y

Cuts at:      0     345    1023 
   Size:        345     678
 
PciI A'CATG_T

Cuts at:      0     341    1023 
   Size:        341     682
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     841    1023 
   Size:        841     182
 
PfoI T'CCnGG_A

Cuts at:      0     896    1023 
   Size:        896     127
 
PpuMI rG'GwC_Cy

Cuts at:      0     880    1023 
   Size:        880     143
 
PsiI TTA'TAA

Cuts at:      0     316    1023 
   Size:        316     707
 
PspOMI G'GGCC_C

Cuts at:      0     382    1023 
   Size:        382     641
 
PstI C_TGCA'G

Cuts at:      0     705    1023 
   Size:        705     318
 
PvuII CAG'CTG

Cuts at:      0     325    1023 
   Size:        325     698
 
SexAI A'CCwGG_T

Cuts at:      0     308    1023 
   Size:        308     715
 
SfcI C'TryA_G

Cuts at:      0     233     368     701    1023 
   Size:        233     135     333     322

  Fragments arranged by size: 

                 333     322     233     135
 
SspI AAT'ATT

Cuts at:      0    1020    1023 
   Size:       1020       3
 
XbaI T'CTAG_A

Cuts at:      0     957    1023 
   Size:        957      66
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     210     762    1023 
   Size:        210     552     261

  Fragments arranged by size: 

                 552     261     210
 

 Enzymes that do cut:

     AarI     AcuI   AflIII    AlwNI     ApaI     ApoI     BanI    BanII
     BbsI    BbvCI Bme1580I     BmrI     BplI   Bpu10I     BsaI    BsaBI
    BsaWI    BsaXI    BseRI    BseYI     BsgI Bsp1286I    BspEI    BspHI
    BspMI    BstXI   Bsu36I     BtsI    CspCI     EaeI  Eco57MI    EcoNI
 EcoO109I    EcoRI    Hin4I     MscI   MspA1I     NspI     PciI    PflMI
     PfoI    PpuMI     PsiI   PspOMI     PstI    PvuII    SexAI     SfcI
     SspI     XbaI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BbeI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmtI     BpmI    BpuEI    BsaAI
    BsaHI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI
  BstZ17I     BtgI    BtgZI     ClaI     DraI   DraIII     DrdI     EagI
     EarI     EciI  EcoICRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PmeI     PmlI     PpiI
    PshAI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     StyI
     SwaI    TaqII     TatI     TstI  Tth111I     XhoI     XmaI     XmnI
     ZraI