(Linear) (Six-base) MAPSORT of: KIEE0405.seq  Check: 9768  from: 1  to: 894



KIEE0405.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AccI GT'mk_AC

Cuts at:      0      16     311     705     894 
   Size:         16     295     394     189

  Fragments arranged by size: 

                 394     295     189      16
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     706     894 
   Size:        706     188
 
AflII C'TTAA_G

Cuts at:      0     432     894 
   Size:        432     462
 
AflIII A'CryG_T

Cuts at:      0     277     708     894 
   Size:        277     431     186

  Fragments arranged by size: 

                 431     277     186
 
ApoI r'AATT_y

Cuts at:      0     456     894 
   Size:        456     438
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      10     202     444     894 
   Size:         10     192     242     450

  Fragments arranged by size: 

                 450     242     192      10
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     810     844     894 
   Size:        810      34      50

  Fragments arranged by size: 

                 810      50      34
 
BfrBI ATG'CAT

Cuts at:      0     622     894 
   Size:        622     272
 
BglI GCCn_nnn'nGGC

Cuts at:      0     818     894 
   Size:        818      76
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     835     894 
   Size:        835      59
 
BsaAI yAC'GTr

Cuts at:      0     278     709     894 
   Size:        278     431     185

  Fragments arranged by size: 

                 431     278     185
 
BseYI C'CCAG_C

Cuts at:      0      68     894 
   Size:         68     826
 
BsmI GAATG_Cn'

Cuts at:      0     622     894 
   Size:        622     272
 
BstZ17I GTA'TAC

Cuts at:      0     312     894 
   Size:        312     582
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      12     894 
   Size:         12     882
 
EarI CTCTTCn'nnn_

Cuts at:      0     797     894 
   Size:        797      97
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     706     835     894 
   Size:        706     129      59

  Fragments arranged by size: 

                 706     129      59
 
HincII GTy'rAC

Cuts at:      0     327     894 
   Size:        327     567
 
NsiI A_TGCA'T

Cuts at:      0     624     894 
   Size:        624     270
 
PfoI T'CCnGG_A

Cuts at:      0     788     894 
   Size:        788     106
 
PmlI CAC'GTG

Cuts at:      0     278     894 
   Size:        278     616
 
PstI C_TGCA'G

Cuts at:      0      14     894 
   Size:         14     880
 
SfcI C'TryA_G

Cuts at:      0      10     894 
   Size:         10     884
 
SmlI C'TyrA_G

Cuts at:      0     432     894 
   Size:        432     462
 
SspI AAT'ATT

Cuts at:      0     168     894 
   Size:        168     726
 

 Enzymes that do cut:

     AccI     AcuI    AflII   AflIII     ApoI     BbsI     BcgI    BfrBI
     BglI     BpmI    BsaAI    BseYI     BsmI  BstZ17I     DrdI     EarI
  Eco57MI   HincII     NsiI     PfoI     PmlI     PstI     SfcI     SmlI
     SspI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI     AgeI     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
    BciVI     BclI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     EaeI     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NspI     PacI     PasI
     PciI    PflMI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     StyI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI